Antioxidative, Anti-Inflammatory, and Anticancer Effects of Purified Flavonol Glycosides and Aglycones in Green Tea

Abstract

(1) Background: Extensive research has focused on flavan-3-ols, but information about the bioactivities of green tea flavonols is limited. (2) Methods: In this study, we investigated the antioxidative, anti-inflammatory, and anticancer effects of flavonol glycosides and aglycones from green tea using in vitro cell models. The fractions rich in flavonol glycoside (FLG) and flavonol aglycone (FLA) were obtained from green tea extract after treatment with tannase and cellulase, respectively. (3) Results: FLG and FLA contained 16 and 13 derivatives, respectively, including apigenin, kaempferol, myricetin, and quercetin, determined by mass spectrometry. FLA exhibited higher radical-scavenging activity than that of FLG. FLG and FLA attenuated the levels of intracellular oxidative stress in neuron-like PC-12 cells. The treatment of RAW 264.7 murine macrophages with FLG and FLA significantly reduced the mRNA expression of inflammation-related genes in a dose-dependent manner. Furthermore, FLG and FLA treatments decreased the viability of the colon adenoma cell line DLD-1 and breast cancer cell line E0771. Moreover, the treatment with FLG or FLA combined with paclitaxel had synergistic anticancer effects on the DLD-1 cell line. (4) Conclusions: Flavonols from green tea exerted beneficial effects on health and may be superior to flavan-3-ols.

Coumaroyl Flavonol Glycosides and More in Marketed Green Teas: An Intrinsic Value beyond Much-Lauded Catechins

“…Recent studies have also found triglycosides of myricetin and have defined their disappearance as a consequence of the fermentation and oxidation processes [46]. Recently, the obtainment of a flavonol glycosides-rich extract named FLG was optimized from an aqueous green tea extract after tannase treatment [47]. Apigenin-C-mono-and diglycosides, as well as some different mono-and diglycosides of flavonols myricetin, quercetin, and kaempferol were detected.…”

Simultaneous Optimal Production of Flavonol Aglycones and Degalloylated Catechins from Green Tea Using a Multi-Function Food-Grade Enzyme

“…GTE was prepared according to the method described by Rha et al [30]. Briefly, GT (Osulloc Farm Corp., Seogwipo, Republic of Korea) was harvested from May-June, 2017, and then processed into a loose tea.…”

“…The numbers 1-13, 14, 15, and 16 indicate flavonol glycosides, myricetin, quercetin, and kaempferol, respectively. Refer to the exact mass and structural information of numbered peaks in previous research[30].…”

Combination of green tea catechins, polysaccharides, and flavonols against urban fine dust particle-induced bronchial damage mediated by modulation of inflammation factors and airway cilia

“…Protective effects of the green tea components against FDP-induced damage Recently, we isolated GTE, fractions rich in FLGs, and CTPs from green tea leaves as bioactive ingredients [45,46]. Because phenolic compounds from various plants have been shown to exert protective effects against FDP-induced oxidative stress and in ammation in skin cells [47,48,49] and because green tea polysaccharides exhibit bene cial antioxidant [50], antitumor [36], and antiaging properties [51], we hypothesized that green tea polyphenols and polysaccharides could also ameliorate FDP-induced cellular damage in the airway.…”

“…The chemical properties of GTE, FLGs, and CTPs were investigated. As shown in Table 1 [46]. In addition, the CTPs included mainly pectic substances and glucosidic macromolecules found in unligni ed cell walls, major components of the middle lamellae in plants [45]; these pectic polysaccharides have various pharmacological properties [30,52,53].…”