Antioxidative capacity of vitamin E, vitamin C and their combination in cryopreserved Bhadavari bull semen

Mittal, Payal; Anand, Mukul; Madan, Arun Kumar; Yadav, Sarita; Kumar, J. Stephen Sampath

doi:10.14202/vetworld.2014.1127-1131

Cited by 27 publications

(31 citation statements)

References 22 publications

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“…Additionally, the morphology and sperm activity would be preserved by the binding of Vitamin E to endoperoxides (Luvoni et al, 2006). Superiority of Vitamin E+C over Vitamin E and C alone was reported by Mittal et al (2014), but with different dose rate in buffalo bull smen. Similar to our finding ascorbic acid plays a role in protecting sperm from ROS was also reported earlier (Buetter, 1993) and maintaining the integrity of sperm cells by preventing oxidative damage to sperm DNA (Fraga et al, 1991) as lipid peroxidation begins at the time of ejaculation rather during semen storage (Blesboi et al, 2005).…”

Section: Resultsmentioning

confidence: 97%

Assessment of antioxidants for preservation of crossbred bull semen in Tris based extender

Rao

Mohanty

Bhakat

2017

IJAR

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Present study was aimed to improve poor quality crossbred bulls semen, as occurrence of poor quality ejaculates are one of the major problems in crossbred bulls. Antioxidants and its combination was tried to overcome such problems. Vitamin E, Vitamin C and Vitamin E+C were supplemented at dose rate of 1mg, 5 mM and 1mg + 5mM per ml respectively in split ejaculates to evaluate semen quality during preservation at refrigerated temperature and cryopreservation. The results showed that semen characteristics were better in antioxidant supplemented ejaculates. Total sperm abnormality were significantly lower (p<0.05) in antioxidant supplemented group. The seminal characteristics of crossbred bull semen showed significantly better (p<0.05) performance on preservation, when fortified with Vitamin E as compared to Vitamin C alone as well as Vitamin E and C in combination. The performance of semen additives, were supportive to semen characteristics especially for Vitamin E, moreover fortification with Vitamin E+C was slightly superior to Vitamin C. It can be concluded that fortification of Vitamin E has beneficial role in semen quality improvement followed by Vitamin E+C and Vitamin C.

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Section: Resultsmentioning

confidence: 97%

Assessment of antioxidants for preservation of crossbred bull semen in Tris based extender

Rao

Mohanty

Bhakat

2017

IJAR

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“…Artificial insemination (AI) through the use of cryopreserved semen is an important tool for successful implementation of breeding program to establish pure line breed of goat [3]. The success of AI largely depends on quality of cryopreserved semen that in turns determines the conception rate [4]. Hence, quality assurance through regular monitoring of semen quality has becomes an important component of freez-thawing protocol.…”

Section: Introductionmentioning

confidence: 99%

Assessment of motion and kinematic characteristics of frozen-thawed Sirohi goat semen using computer-assisted semen analysis

Anand¹,

Yadav²

2016

Vet World

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Aim:The aim was to determine the motion and kinematics characteristic of frozen-thawed spermatozoa in Sirohi goat using computer-assisted semen analysis. Materials and Methods:A study was carried out in Sirohi buck. Semen collection was made biweekly from each buck with the help of artificial vagina. A total of 12 ejaculates were collected from two bucks (six ejaculates from each buck). Freshly collected semen was pooled and later evaluated. The pooled semen sample was extended with standard glycerolated egg yolk tris extender and later subjected to a process of cryopreservation. The motion and kinematic characteristics of spermatozoa were studied during freez-thawing process.Results: Significantly (p<0.01) higher value of live percent, hypo-osmotic swelling test, and acrosomal integrity were recorded in neat semen followed by diluted and frozen thaw semen. The proportion of spermatozoa showing slow progression were the highest in the neat and diluted semen followed by rapid and non-progressively motile, while a reverse pattern was observed in the frozen thaw semen where the proportion of non-progressively motile spermatozoa were significantly (p<0.01) higher followed by slow and rapid progression. Conclusion:This study showed that the best results for motion, vitality, plasma membrane integrity, and acrosome status were obtained in the neat semen followed by diluted and frozen thaw semen. Further, the process of cryopreservation results in a shift of motility from slow to non-progressive in the post-thaw semen with a significant decrease in the path velocities when compared to neat and diluted semen. Hence, it can be concluded that freezing-thawing process reduces the motility and kinematic characters spermatozoa and may be an important factor affecting the fertilizing ability of spermatozoa resulting in poor conception rate after insemination in goats.

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“…Increase in motility and viability of spermatozoa in the present investigation might be due to the antioxidant properties of ascorbic acid. Ascorbic acid due to its antioxidant properties prevents the toxic effect to spermatozoa which occur due to hydrogen peroxide release during the cryopreservation and hence the spermatozoa remain viable for a long period but not protected [ 17 , 21 ].…”

Section: Resultsmentioning

confidence: 99%

Effect of different antioxidant additives in semen diluent on cryopreservability (−196°C) of buffalo semen

Patel¹,

Siddiquee

Chaudhari

et al. 2016

Vet World

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Aim:The aim of this study was to evaluate the effect of different antioxidant additives in standard tris-fructose-egg yolk-glycerol (TFYG) extender on the cryopreservability of buffalo semen.Materials and Methods:Semen collection using artificial vagina, twice weekly for 5 weeks from three pedigreed health breeding bulls of Mehsani breed, aged between 6 and 8 years. Immediately after initial evaluation all 30 qualifying ejaculates (10/bull) were split into three aliquots and diluted at 34°C keeping the concentration of 100 million spermatozoa/ml with standard TFYG extender as control and TFYG having two antioxidant additives - Cysteine HCl at 1 mg/ml and ascorbic acid at 0.2 mg/ml to study their comparative performance. Semen filled in French Mini straws using IS-4 system and gradually cooled to 4°C and equilibrated for 4 h in cold handing cabinet. After completion of equilibration, straws were cryopreserved in LN2 by Programmable Bio-freezer. Semen was examined at post-dilution, post-equilibration, and post-thaw stages for sperm quality parameters, and at each stage plasma was separated for enzymatic analysis of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and alkaline phosphatase (AKP).Results:The mean percentage of sperms in TFYG, TFYG + cysteine HCl and TFYG + ascorbic acid diluents at post-thaw stage in terms of progressive motility (52.83±0.52, 57.83±0.52, 57.83±0.52), livability (78.70±0.21, 82.33±0.23, 81.73±0.22), and abnormality (5.43±0.21, 5.03±0.17, 5.23±0.18) varied significantly (p<0.05) between control TFYG and TFYG having antioxidant additives. The mean U/L activities of AST (78.70±0.47, 72.80±0.48, 73.30±0.54), LDH (172.70±0.41, 155.78±0.42, 156.33±0.41), and AKP (103.61±0.34, 90.20±0.34, 91.03±0.34) in semen diluted with TFYG, TFYG + cysteine HCl and TFYG + ascorbic acid diluents at post-thaw stage, respectively, which showed significantly (p<0.05) higher leakage of enzymes in control TFYG than TFYG incorporated with additives.Conclusion:Incorporation of antioxidant additives such as cysteine HCl and ascorbic acid in standard TFYG diluents improves sperm quality parameters, reduces enzyme leakage, and ultimately advances cryopreservability of buffalo semen.

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Antioxidative capacity of vitamin E, vitamin C and their combination in cryopreserved Bhadavari bull semen

Cited by 27 publications

References 22 publications

Assessment of antioxidants for preservation of crossbred bull semen in Tris based extender

Assessment of antioxidants for preservation of crossbred bull semen in Tris based extender

Assessment of motion and kinematic characteristics of frozen-thawed Sirohi goat semen using computer-assisted semen analysis

Effect of different antioxidant additives in semen diluent on cryopreservability (−196°C) of buffalo semen

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