Antioxidative effects of magnetized extender containing bovine serum albumin on sperm oxidative stress during long-term liquid preservation of boar semen

Lee, Sang-Hee; Park, Choon‐Keun

doi:10.1016/j.bbrc.2015.06.159

Cited by 20 publications

(13 citation statements)

References 38 publications

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“…Furthermore, it has been proposed that ELEF can increase the temperature of the freezing media by increasing the speed of the thawing process which consequently, reduced the probability of re-crystallization of the ice around of the sperm leading to less cryo-injury to the sperm membrane. Our findings in this parts are in agreement with Lee et al 2015 who reported that magnetized extender improves the viability and fertilizing ability of boar sperm during a long-term liquid storage [37, 50]. However, there is much less known about the beneficial effects of EMFs exposure to the reproductive performance and the semen quality [51–57].…”

Section: Discussionsupporting

confidence: 91%

Electromagnetic field in human sperm cryopreservation improves fertilizing potential of thawed sperm through physicochemical modification of water molecules in freezing medium

et al. 2019

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Physicochemical properties of water molecules as the main compositions of the freezing media can be affected by the electromagnetic fled. The purpose of this study was to apply extremely low repetition rate electromagnetic fields (ELEFs) to change the molecular network of water molecules existing in freezing media used for human sperm cryopreservation. First, different time periods and pulsed electromagnetic fields were used to evaluate the physiochemical properties of water. The lowest rate of cluster size, surface tension, viscosity, and density was observed for water samples exposed to 1000 Hz ELEF for 60 min (P < 0.05) that could be results in small ice crystal formation. Therefore, this treatment was selected for further evaluations in human sperm freezing because there was minimal probability of amorphous ice crystallization in this group. To assess fertilizing potential, human semen samples were subjected to ELEF (1000 Hz) water-made freezing medium and cryopreserved. The highest percentage of total motility, progressive motility, viability, membrane integrity, mitochondrial membrane potential, DNA integrity, and TAC were obtained in frozen ELEF as compared to other groups. The percentage of viable spermatozoa (Annexin V - /PI - ) in frozen ELEF was significantly higher than in frozen control. The level of ROS was significantly lower in frozen ELEF when compared to frozen control. It can be concluded that the modification of physicochemical properties of water existing in cryopreservation media by ELEF is a suitable strategy to improve the outcome of cryopreservation.

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Section: Discussionsupporting

confidence: 91%

Electromagnetic field in human sperm cryopreservation improves fertilizing potential of thawed sperm through physicochemical modification of water molecules in freezing medium

et al. 2019

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“…In vitro embryo production is widely used in animals, especially cattle, to reduce the interval between generations. As with standard breeding by animal traits, obtaining high‐quality offspring requires high‐quality gametes (Lee and Park, ). Gametes and embryos handled in vitro are often exposed to excessive oxygen during their manipulation, which can lead to supra‐physiological levels of reactive oxygen species (ROS) (Cheuqueman et al, ).…”

Section: Introductionmentioning

confidence: 99%

Protective effects of melatonin on bovine sperm characteristics and subsequent in vitro embryo development

Pang

Sun

Jiang

et al. 2016

Molecular Reproduction Devel

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We aimed to investigate the effect of melatonin on bovine frozen-thawed semen and its impact on fertilization outcome. Plasma membrane integrity, mitochondrial activity, acrosome integrity, and levels of intracellular reactive oxygen species (ROS) were measured in spermatozoa treated with different concentrations of melatonin. Melatonin-treated spermatozoa were then used for in vitro fertilization, followed by analysis of subsequent embryo development and the expression of apoptosis- and antioxidant-related genes. The results revealed that 10 and 10 M melatonin led to higher plasma membrane integrity, mitochondrial activity, and acrosome integrity, and significantly decreased intracellular ROS levels (P < 0.05). The blastocyst development rate of in vitro-produced bovine embryos originating from 10 M melatonin-treated spermatozoa was significantly higher, while the incidence of apoptotic nuclei in blastocysts was markedly lower than for embryos from any other group (P < 0.05). CASP3 and BAX mRNA abundance were significantly reduced whereas BCL2, XIAP, and CAT transcript abundance were significantly increased in embryos produced from spermatozoa treated with 10 M melatonin; GPX4 expression, however, was comparable in all treatment groups. Thus, 10 M melatonin can improve the quality of bovine frozen-thawed semen. These beneficial effects appear to influence preimplantation embryos, given the correlation with its anti-apoptotic and anti-oxidative properties. Mol. Reprod. Dev. 83: 993-1002, 2016 © 2016 Wiley Periodicals, Inc.

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“…Previous studies have demonstrated that sperm motility, acrosome integrity, and lipid peroxidation are more sensitive indicators of oxidative stress [ 37 , 38 ], and ROS levels can be managed by the addition of ROS-scavenging antioxidants [ 39 ]. Therefore, the protection of spermatozoa against oxidative stress by antioxidant supplementation is currently pursued [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

C60 Fullerenes Suppress Reactive Oxygen Species Toxicity Damage in Boar Sperm

Liu

et al. 2019

Nano-Micro Lett.

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HIGHLIGHTS • Carboxylated C60 may be considered a novel antioxidant agent to be used as a semen extender supplement for assisted reproductive technology. • Carboxylated C60 could enhance the motility parameters and characteristics of boar spermatozoa during liquid storage at 4 °C and protect boar sperm from oxidative stress by inhibiting the reactive-oxygen-species-induced protein dephosphorylation. • Novel insights into the molecular mechanisms contributing to the protective effects of carboxylated C60 are provided.

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Antioxidative effects of magnetized extender containing bovine serum albumin on sperm oxidative stress during long-term liquid preservation of boar semen

Cited by 20 publications

References 38 publications

Electromagnetic field in human sperm cryopreservation improves fertilizing potential of thawed sperm through physicochemical modification of water molecules in freezing medium

Electromagnetic field in human sperm cryopreservation improves fertilizing potential of thawed sperm through physicochemical modification of water molecules in freezing medium

Protective effects of melatonin on bovine sperm characteristics and subsequent in vitro embryo development

C60 Fullerenes Suppress Reactive Oxygen Species Toxicity Damage in Boar Sperm

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