Antioxidative Effects of Standardized Aronia melanocarpa Extract on Reproductive and Metabolic Disturbances in a Rat Model of Polycystic Ovary Syndrome

Rudic, Jovan; Jakovljević, Vladimir; Jovic, Nikola; Nikolić, Milutin; Sretenovic, Jasmina; Mitrović, Slobodanka; Bolevich, Sergey; Bolevich, Stefani; Mitrović, Miroslav; Raičević, Saša; Andrić, Kristina; Milenkovic, Andjela Dimkic; Rakic, Dejana; Joksimović, Jovana

doi:10.3390/antiox11061099

Cited by 9 publications

(8 citation statements)

References 68 publications

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“…A similar trend was recorded in the clomiphene and metformin groups or in the combination of KH with both drugs. In agreement with previous studies [ 62 , 70 , 71 ], metformin and clomiphene were reported to reverse the elevated testosterone level in letrozole-induced PCOS rats. In women with PCOS, metformin causes a rapid decrease in LH-stimulated testosterone secretion [ 72 ].…”

Section: Discussionsupporting

confidence: 93%

Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats

et al. 2022

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Kelulut honey (KH) has been proven to have excellent antioxidative and anti-inflammatory properties with unique physicochemical characteristics. Therefore, we investigated the isolated and combined effects of KH, metformin, or clomiphene in alleviating oxidative stress and reproductive and metabolic abnormalities in polycystic ovary syndrome (PCOS). Female Sprague-Dawley (SD) rats were given 1 mg/kg/day of letrozole for 21 days to induce PCOS. PCOS rats were then divided into six treatment groups: untreated, metformin (500 mg/kg/day), clomiphene (2 mg/kg/day), KH (1 g/kg/day), combined KH (1 g/kg/day) and metformin (500 mg/kg/day), and combined KH (1 g/kg/day) and clomiphene (2 mg/kg/day). All treatments were administered orally for 35 days. The physicochemical characteristics of KH were assessed through hydroxymethylfurfural, free acidity, diastase number, moisture content, sugar profile, metals, and mineral compounds. Additionally, we determined the semivolatile organic compounds present in KH through gas chromatography-mass spectrometry (GC/MS) analysis. KH and its combination with metformin or clomiphene were shown to improve the oestrus cycle, hormonal profile, and oxidative stress in PCOS rats. However, KH did not reduce the fasting blood glucose, insulin, and body weight gain in PCOS rats. These findings may provide a basis for future studies to discover the potential use of KH as a complementary treatment for women with PCOS.

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Section: Discussionsupporting

confidence: 93%

Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats

et al. 2022

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“…However, metformin treatment did not affect the antral follicles count in PCOS rats. Similar findings were observed in the past on the metformin effect on PCOS rat’s ovarian histology [ 65 ]. Interestingly, the combination of metformin with KH significantly increased the antral follicle and corpus luteum counts and reduced the cystic follicle count.…”

Section: Discussionsupporting

confidence: 90%

“…Interestingly, the combination of metformin with KH significantly increased the antral follicle and corpus luteum counts and reduced the cystic follicle count. Rudic et al revealed that the combination of Aronia melanocarpa with metformin reduced the cystic follicle count and increased the corpus luteum count in PCOS rats [ 65 ]. Meanwhile, the combination of KH with clomiphene recorded the highest restoration of corpus luteum and antral follicles compared to all other treatment groups.…”

Section: Discussionmentioning

confidence: 99%

Kelulut Honey Improves Folliculogenesis, Steroidogenic, and Aromatase Enzyme Profiles and Ovarian Histomorphology in Letrozole-Induced Polycystic Ovary Syndrome Rats

Kamal

Ibrahim²,

Ugusman³

et al. 2022

Nutrients

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Polycystic ovary syndrome (PCOS) has been linked to aberrant folliculogenesis and abnormalities in the aromatase enzyme (Cyp19a1) and the steroidogenic enzyme, 17-alpha-hydroxylase (Cyp17a1) expression. It has been demonstrated that Kelulut honey (KH) improves both female and male reproductive system anomalies in animal studies. Here, we examined the effects of isolated and combined KH, metformin, and clomiphene in improving folliculogenesis, aromatase, and steroidogenic enzyme profiles and ovarian histomorphology in letrozole-induced PCOS rats. Letrozole (1 mg/kg/day) was administered to female Sprague–Dawley (SD) rats for 21 days to induce PCOS. PCOS rats were subsequently divided into six experimental groups: untreated, treatment with metformin (500 mg/kg/day), clomiphene (2 mg/kg/day), KH (1 g/kg/day), combined KH (1 g/kg/day) and metformin (500 mg/kg/day), and combined KH (1 g/kg/day) and clomiphene (2 mg/kg/day). All treatments were given orally for 35 days. We found that KH was comparable with clomiphene and metformin in improving the expression of Cyp17a1 and Cyp19a1, apart from enhancing folliculogenesis both histologically and through the expression of folliculogenesis-related genes. Besides, the combination of KH with clomiphene was the most effective treatment in improving the ovarian histomorphology of PCOS rats. The effectiveness of KH in restoring altered folliculogenesis, steroidogenic, and aromatase enzyme profiles in PCOS warrants a future clinical trial to validate its therapeutic effect clinically.

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“…Uzunsoy / Uluslararası Tarım Araştırmalarında Yenilikçi Yaklaşımlar Dergisi / International Journal of Innovative Approaches in Agricultural Research, 2022, Vol. 6 (4) Kähkönen, Hopia & Heinonen, 2001;Wu, Gu, Prior & McKay, 2004;Osmiańzski & Wojdylo, 2005;Jakobek, Šeruga & Krivak, 2011;Kapci, 2013;Tolić et al, 2015;Szopa et al 2017;Dąbrowska et al, 2019;Denev, Číž, Kratchanova & Blazheva, 2019;Rudic et al, 2022Brӓunlich et al, 2013Denev et al, 2019;Daoutidou, Plessas, Alexopoulos & Mantzourani, 2021;Deng et al, 2021Ohgami et al, 2005Martin et al, 2014;Kang et al, 2017;Lee et al, 2018;Jang, Lee, Choi & Yim, 2020;Ali et al, 2021;Li et al, 2022Bhaswant, Shafie, Mathai, Mouatt & Brown, 2017Jeon et al, 2018;Jeong & Kim, 2019;Chen, Zhu & Meng, 2020;Mu et al, 2020Xie et al, 2017Kim et al, 2018;Jeong & Kim, 2019;Tasic et al, 2021Bell & Gochenaur, 2006Naruszewicz, Laniewska, Millo & Dłuzniewski, 2007;Olas et al, 2008;Ćujić et al, 2018;Istas et al, 2019Malik et al, 2003Lala et al, 2006;Kędzierska et al, 2013;Choi et al, 2018a;Thi & Hwang, 2018;…”

Section: Phenolic Content Of Aronia and Related Antioxidant Activitymentioning

confidence: 99%

Bioactivity of Aronia Products, and The Promising Use of Aronia in Dairy Industry

Uzunsoy¹

2022

IJIAAR

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In recent years, foods are not only valued in terms of taste and nutritional value, but also preferred in terms of post-consumption health effects. In particular, the trend towards functional foods that offer health benefits beyond their nutritional value to consumers has increased. Dairy products have a very important place among functional foods, and fruit-added dairy products like fermented milk, kefir and yogurt etc. lead the way due to the consumption preferences. Aronia (Aronia melanocarpa) is a forest fruit originating from North America, but it is grown in many parts of Europe lately. The fruit is very rich in proanthocyanins, anthocyanins, procyanidin, phenolic acids, flavonols and flavanones. It is known as the fruit with the highest antioxidant activity, and shows antidiabetic, anti-inflammatory, antiviral, antibacterial, hypotensive, cardioprotective, hepatoprotective and anticarcinogenic properties. The fruit is mostly used as ingredient in wine, marmalade, fruit juice, tea, extract and as dietary supplement or food colorant. The health-promoting effects of aronia due to its bioactivity are enhanced with the functional dairy products, and lately aronia-added yogurt and kefir are industrially manufactured and marketed for dairy consumers. This review focuses on the health effects of aronia and the promising use of the fruit in dairy products.

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Antioxidative Effects of Standardized Aronia melanocarpa Extract on Reproductive and Metabolic Disturbances in a Rat Model of Polycystic Ovary Syndrome

Cited by 9 publications

References 68 publications

Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats

Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats

Kelulut Honey Improves Folliculogenesis, Steroidogenic, and Aromatase Enzyme Profiles and Ovarian Histomorphology in Letrozole-Induced Polycystic Ovary Syndrome Rats

Bioactivity of Aronia Products, and The Promising Use of Aronia in Dairy Industry

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