Antioxidative enzyme and glutathione S-transferase activities in diabetic rats exposed to long-term ASA treatment

Лапшина, Е. А.; Sudnikovich, E. Yu.; Maksimchik, Ju.Z.; Zabrodskaya, S. V.; Zavodnik, L.B.; Kubyshin, V.L.; Nocuń, Marek; Kaźmierczak, Piotr; Dobaczewski, Marcin; Watała, Cezary; Заводник, И. Б.

doi:10.1016/j.lfs.2006.06.008

Cited by 30 publications

(27 citation statements)

References 41 publications

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“…Light treatment was ineffective at reversing hepatic CAT or GST activity. Our results from the study of chronic diabetes are in good agreement with other antioxidant studies that treated rats longer than 3 weeks with isoeugenol [33], quercetin [34], piperine [35], coenzyme Q 10 [36], vanadate [37], acetylsalicylic acid (ASA) [38], and various other antioxidants [32]. These studies also did not report improvement in hepatic CAT activity.…”

Section: Discussionsupporting

confidence: 90%

Effects of low‐level light therapy on hepatic antioxidant defense in acute and chronic diabetic rats

Lim

Ali

Sanders

et al. 2009

J Biochem & Molecular Tox

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Diabetes causes oxidative stress in the liver and other tissues prone to complications. Photobiomodulation by near infrared light (670 nm) has been shown to accelerate diabetic wound healing, improve recovery from oxidative injury in the kidney, and attenuate degeneration in retina and optic nerve. The present study tested the hypothesis that 670 nm photobiomodulation, a low-level light therapy, would attenuate oxidative stress and enhance the antioxidant protection system in the liver of a model of type I diabetes. Male Wistar rats were made diabetic with streptozotocin (50 mg/kg, ip) then exposed to 670 nm light (9 J/cm(2)) once per day for 18 days (acute) or 14 weeks (chronic). Livers were harvested, flash frozen, and then assayed for markers of oxidative stress. Light treatment was ineffective as an antioxidant therapy in chronic diabetes, but light treatment for 18 days in acutely diabetic rats resulted in the normalization of hepatic glutathione reductase and superoxide dismutase activities and a significant increase in glutathione peroxidase and glutathione-S transferase activities. The results of this study suggest that 670 nm photobiomodulation may reduce, at least in part, acute hepatic oxidative stress by enhancing the antioxidant defense system in the diabetic rat model.

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Section: Discussionsupporting

confidence: 90%

Effects of low‐level light therapy on hepatic antioxidant defense in acute and chronic diabetic rats

Lim

Ali

Sanders

et al. 2009

J Biochem & Molecular Tox

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“…In addition, a decrease in serum glucose level can be another reason for reduced AGEs and HbA1c. Similar changes in the AGEs (Urios et al, 2007) and HbA1c (Lapshina et al, 2006) have been reported elsewhere.…”

Section: Discussionsupporting

confidence: 88%

Investigation of the Mechanisms Involved in the High-Dose and Long-Term Acetyl Salicylic Acid Therapy of Type I Diabetic Rats

Jafarnejad¹,

Bathaie²,

Nakhjavani³

et al. 2007

J Pharmacol Exp Ther

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Diabetes mellitus has been classified as a conformational disease because of changes induced in the structure and function of proteins due to hyperglycemia. In this study, we investigated the effect of high-dose and long-term use of acetyl salicylic acid (ASA) on the streptozotocin-induced diabetic rats as a model of type I diabetes, with consideration on the structure and/or function of proteins. The N-[methylnitrosocarbamoyl]-D-glucosamine (streptozotocin)-induced diabetic rats together with the normal rats were studied for 5 months with and without receiving 100 mg/kg ASA in drinking water. All rats were investigated from different aspects such as heat shock protein (HSP) 70 level, serum glucose and insulin concentration, advanced glycated end product (AGE) and glycated hemoglobin (HbA1c) formation, lipid profile, high-density lipoprotein (HDL) functionality (paraoxonase1 and lecithin cholesterol acyltransferase activities), and the antioxidant system. In addition, the in vitro effect of ASA on the structure of albumin as a model protein was studied in the presence of glucose by spectroscopic techniques such as fluorometry and circular dichroism. The results show that ASA therapy causes a decrease in the glucose level and AGE and HbA1c formation, improves the lipid profile, HDL functionality, and the antioxidant capacity, induces serum HSP70, and overall decreases mortality of diabetic rats in comparison with the group without treatment. The conformation of glycated bovine serum albumin is different from the native form, and ASA retains the conformation of this protein similar to the native. The improving effect of ASA on diabetic rats is mostly due to its role as a chemopreventive agent in the structural conservation and protection of proteins involved in diabetes pathogenesis.

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“…Glutathione S transferase is an antioxidant protein, whose activity gets reduced in long-term hyperglycemia. Perhaps, the decrease in the activity of glutathione S transferase could be due to glycation [24,25]. The activity of perroxiredoxin has been shown to increase in diabetic condition, in our study the protein was found to be glycated [6].…”

Section: Identification Of Glycated Proteinssupporting

confidence: 51%

Proteomic Analysis of Glycated Proteins from Streptozotocin-Induced Diabetic Rat Kidney

et al. 2011

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Glycation of proteins leading to formation of advanced glycation end products (AGEs) has been considered as one of the important causes of diabetic nephropathy. Therefore, in this study, glycated proteins were detected by anti-AGE antibodies from kidney of streptozotocin-induced diabetic rat showing nephropathic symptoms, by using two dimensional electrophoresis and western blot analysis. These glycated proteins were identified and characterized by using combination of peptide mass finger printing and tandem mass spectrometric approaches. Glycated proteins identified included proteins from metabolic pathways, oxidative stress, cell signaling, and transport. Several of the proteins modified by glycation were involved in glucose metabolism. The extent of glycation was higher in diabetes compared to control, in the glycated proteins that were common to both control and diabetic kidney. Two dimensional electrophoresis proteins profiling of glycated proteins suggest that four of the glycated proteins were significantly up regulated in diabetes.

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Antioxidative enzyme and glutathione S-transferase activities in diabetic rats exposed to long-term ASA treatment

Cited by 30 publications

References 41 publications

Effects of low‐level light therapy on hepatic antioxidant defense in acute and chronic diabetic rats

Effects of low‐level light therapy on hepatic antioxidant defense in acute and chronic diabetic rats

Investigation of the Mechanisms Involved in the High-Dose and Long-Term Acetyl Salicylic Acid Therapy of Type I Diabetic Rats

Proteomic Analysis of Glycated Proteins from Streptozotocin-Induced Diabetic Rat Kidney

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