Antioxidative mechanism involved in the preventive efficacy ofBergenia ciliatarhizomes against experimental nephrolithiasis in rats

Abstract

Context: Bergenia ciliata Haw. (Saxifragaceae) is widely used in traditional medicines for renal disorders including kidney stones, inflammation and also well known for its antioxidant activity. Use of traditional herbs proved to be an important strategy for the management of kidney stones by modulating the oxidative stress imposed by calcium oxalate nephrolithiasis. Objectives: To evaluate the antinephrolithiatic and antioxidative activity of B. ciliata rhizomes as a preventive agent on ethylene glycol (EG)-induced nephrolithiasis. Materials and methods: The hydro-methanol extract (30:70, v/v) of B. ciliata rhizomes was orally administrated simultaneously at a dose of 150 and 300 mg/kg body weight/day, to adult female Wistar rats for 28 d along with EG (0.75%, v/v) in drinking water. The results were compared to a parallel study conducted with marketed polyherbal drug cystone under identical dosage conditions. The biochemical parameters were measured in urine, serum and kidney followed by histochemistry. A validated HPLC method was used for standardization using gallic acid as a marker.Results: EG caused a significant increase in calcium, oxalate and phosphate levels in urine and kidney and concurrent decrease in calcium, sodium and magnesium in serum (p50.001). EG also caused an increase in lipid peroxidation and a decrease in activities of antioxidative enzymes in kidney. Co-treatment with B. ciliata rhizomes extract caused restoration of all these parameters (p50.001). Histochemical studies showed reduced calcifications with extract treatment. Conclusion: B. ciliata has a significant prophylactic effect in preventing the nephrolithiasis, which might be mediated through antioxidant activity of these active compounds.

Aqueous Cannabis Extract Prevents Ethylene Glycol-induced Renal Calcium Crystallization

“…properties, is used in traditional medicine for treating renal disorders, including kidney stones. This study also showed reduced calcifications following extract treatment (26). In conclusion, these data indicate that a Cannabis sativa extract can prevent crystallization in urolithiasis rats.…”

Bergenin from Bergenia Species Produces a Protective Response against Myocardial Infarction in Rats

“…However, some endogenous antioxidants, such as glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT), provide a first-line defense against the free radicals [44,45]. Previous studies reported that bergenin and the plant sources Bergenia ciliate, Bergenia ligulata Wall, and Ficus racemose L. significantly restore the SOD, GPX, and CAT enzymes' activities [46][47][48]. It is also reported that bergenin exhibits a significant activity against free radicals [13].…”

Bergenia ciliata as antibacterial agent

“…Boiled roots of Bergenia ciliata in combination with salt have been reported to be used for the cure of asthma. Its use for the treatment and prevention of piles and cancer is also reported [7,19,20].…”