Antioxidative responses of cell suspension cultures of two Coffea arabica varieties to low aluminum levels at pH 5.8

Abstract

-(Antioxidative responses of cell suspension cultures of two Coffea arabica varieties to low aluminum levels at pH 5.8). The effects of aluminum (Al) on the activities of antioxidant enzymes and ferritin expression were studied in cell suspension cultures of two varieties of Coffea arabica, Mundo Novo and Icatu, in medium with pH at 5.8. The cells were incubated with 300 μM Al 3+ , and the Al speciation as Al 3+ was 1.45% of the mole fraction. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) were increased in Mundo Novo, whereas glutathione reductase (GR) and guaiacol peroxidase (GPOX) activities remained unchanged. SOD, GR, and GST activities were increased in Icatu, while CAT activity was not changed, and GPOX activity decreased. The expression of two ferritin genes (CaFer1 and CaFer2) were analyzed by Real-Time PCR. Al caused a downregulation of CaFER1 expression and no changes of CaFER2 expression in both varieties. The Western blot showed no alteration in ferritin protein levels in Mundo Novo and a decrease in Icatu. The differential enzymes responses indicate that the response to Al is variety-dependent.

Bacillus Subtilis No Controle Biológico Da Ferrugem E Do Bichomineiro No Cafeeiro

“…*comparada à testemunha.DISCUSSÃOA maior resistência da cultivar Icatu também foi evidenciada porMontes et al (2012), onde na avaliação da ferrugem no cafeeiro, a severidade da doença foi menor nas cultivares Icatu e Obatã. Essa cultivar é um híbrido obtido do cruzamento de Coffea arabica e Coffea canephora, tendo herdado de C. canephora a resistência maior a ferrugem(BOTTCHER et al, 2012) Martins et al (2004),. comparando agroecossistemas plantados com cultivares resistentes e suscetíveis à ferrugem em Minas Gerais, observaram que a cultivar Icatu não atingiu o nível de dano da doença, ao contrário da outras cultivares suscetíveis que ultrapassaram o nível de dano.…”