Antioxidative role of selenium against the toxic effect of heavy metals (Cd+2, Cr+3) on liver of rainbow trout (Oncorhynchus mykiss Walbaum 1792)

Abstract

The main purpose of this study is to discuss the effect of Cd+2, Cr+3 and Se metals on biochemical parameters in liver tissue of Oncorhynchus mykiss. The rainbow trout were exposed to heavy metal stress (Cd+2, Cr+3) at 2 ppm dosage. The present study was undertaken to determine the protective effect of selenium treatment at the same dosage (2 ppm) on some biochemical parameters. The activity of catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and the changes in levels of malondialdehyde (MDA) from biochemical parameters were determined in liver tissue of the fish groups exposed to heavy metals, especially for the selenium-applied groups. Results of this study showed that the activities of CAT, GSH-Px and SOD in the tissues of fish exposed to the stress of Cd+2 and Cr+3 were significantly lower than the control groups (P < 0.05). Meanwhile, the closer values to the control groups were obtained in selenium-added groups (Cr+3 + Se+4, Cd+2 + Se+4). For the level of MDA, the last production of lipid peroxidation showed increases (P < 0.05) in the groups exposed to the metal stress, whereas significant decreases were obtained in selenium-applied groups. The result of the statistical evaluation showed that the negative effects occurring in the biochemical parameters of the applied groups exposed to the toxicity of heavy metal were significantly eliminated (P < 0.05) as a result of selenium treatment.

Responses of antioxidant enzymes, lipid peroxidation, and Na+/K+-ATPase in liver of the fish Goodea atripinnis exposed to Lake Yuriria water

“…GPx inhibition was also reported by Oruç and Ü ner (2000) in livers of the Nile tilapia Oreochromis niloticus after combined treatment with the herbicide 2,4-dichlorophenoxyacetic acid and the insecticide azinphosmethyl. Furthermore, Talas et al (2008) observed a marked depletion in GPx activity in the liver of Oncorhynchus mykiss after exposure to Cd and Cr. Pandey et al (2001) detected a decrease in GPx and GST activities after exposure to Cu in Channa punctatus, and Dupuy and Szabo (1986) documented significant decreases in rat cysteine levels following Cu exposure.…”

Biochemical changes and sensory assessment on tissues of carp (Cyprinus carpio, Linnaeus 1758) during sale conditions

“…Negative correlations were found between CAT activity and MDA levels. Lipid peroxidation, considered a complex selfpropagating process producing high levels of cell degradation, increases the rigidity (decreases the fluidity) of cellular membranes (Ates et al 2008;Talas et al 2008). Table 1 shows the results of the biochemical analysis of the carp stored at the selling conditions (9-12°C) in the laboratory in relation to storage time post-slaughter.…”

The effects of organic selenium on performance and oxidative level in rainbow trout (<i>Oncorhynchus mykiss</i>, Walbaum, 1792) fed a high-fat diet

“…An increase in the fat content of the diet may lead to a higher level of non-enzymatic antioxidants, thereby increase the Se requirement. Moreover, most previous experiments in dietary Se requirements for rainbow trout and Atlantic salmon were conducted using mineral Se (Hilton et al, 1980;Bell et al, 1986Bell et al, , 1987Talas et al, 2008). This has been reported to have lower bioavailability and trophic transfer properties compared with organic Se (selenomethionine) (Lorentzen et al, 1994;Wang and Lovell, 1997;Fan et al, 2002).…”