Antioxidative stress effect of phosphoserine dimers is mediated via activation of the Nrf2 signaling pathway

Peng

J of Cellular Biochemistry

et al. 2019

Clinically, bone marrow mesenchymal stem cells (BMSCs) have been used in treatment of many diseases, but the local oxidative stress (OS) of lesion severely limits the survival of BMSCs, which reduces the efficacy of BMSCs transplantation. Therefore, enhancing the anti-OS stress ability of BMSCs is a key breakthrough point. Preconditioning is a common protective mechanism for cells or body. Here, the aim of this study was to investigate the effects of OS preconditioning on the anti-OS ability of BMSCs and its mechanism.Fortunately, OS preconditioning can increase the expression of superoxide dismutase, catalase, NQO1, and heme oxygenase 1 through the nuclear factor erythroid 2-related factor 2 pathway, thereby decreased the intracellular reactive oxygen species (ROS) levels, relieved the damage of ROS to mitochondria, DNA and cell membrane, enhanced the anti-OS ability of BMSCs, and promoted the survival of BMSCs under OS.

Section: Discussionmentioning

confidence: 99%

New strategy of bone marrow mesenchymal stem cells against oxidative stress injury via Nrf2 pathway: oxidative stress preconditioning

Peng

J of Cellular Biochemistry

et al. 2019

“…). Our results support the previous finding that pro‐inflammatory cytokine productions such as IL‐8 were upregulated by activation of MAPK and NF‐κB inflammatory signalling pathways in intestinal epithelial cells (Jijon et al ., ; Mine et al ., ). Due to both NF‐κB and the MAPK pathways were suppressed by SS‐2 supplementation, our current results suggest that SS‐2 is able to interfere with inflammatory signalling transductions that possibly occur at their upper stream.…”

Section: Resultsmentioning

confidence: 99%

Effects of a synthetic di‐phosphoserine peptide (SS‐2) on gene expression profiling against TNF‐α induced inflammation

Mine

2019

Int J of Food Sci Tech

Self Cite

Summary It has been showed bioactive di‐phosphoserine peptide (SS‐2) possesses functions to ameliorate oxidative stress in vitro. This study aimed to substantiate the role of bioactive di‐phosphoserine peptide (SS‐2) in modulating inflammatory responses in TNF‐α‐stimulated HT‐29 cells, and its mechanism of action. SS‐2 significantly reduced IL‐8 secretion in TNF‐α‐induced HT‐29 cells, and also suppressed pro‐inflammatory cytokines, including IL‐8, IL‐12, MCP‐1 and TNF‐α. Moreover, SS‐2 inhibited TNF‐α initiated signalling cascades by suppressing phosphorylation of the ERK1/2, JNK, P38 and IκB those culminate in above cellular inflammatory responses. Differentially expressed genes analysis within NF‐κB signalling pathway revealed that SS‐2 blocks multiple sites of upstream NF‐κB signalling cascade, including FADD and MyD88, thereby preventing the signalling transduction involved in cellular inflammatory response. These results provide a new insight into molecular mechanism for anti‐inflammatory action of SS‐2, suggesting SS‐2 is a potential alternative approach to treat IBD by particular targeting TNF‐α driven inflammatory event.

Intern J of Cosmetic Sci

“…Another novel mechanism through which theophylline may be of benefit as an anti‐skin ageing agent is its stimulatory effect on the expression of metallothionein in human epidermis. Metallothioneins are low molecular weight cysteine‐rich proteins that are also rich in metal content, some of which are ubiquitously expressed, and are highly induced in response to different environmental stressors including metal ions cytokines, and free radicals . They play a role in cell cycle regulation and may protect cells from apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Theophylline exerts complex anti‐ageing and anti‐cytotoxicity effects in human skin ex vivo

Bertolini

Ramot

Gherardini

et al. 2019

OBJECTIVE: Theophylline is a phosphodiesterase inhibitor that is being used clinically for asthma therapy. In addition, it is recognized as a cosmetic agent with possible anti-ageing and anti-oxidative properties. Nevertheless, how it affects human skin is still poorly examined. METHODS: Theophylline (10 or 100 µM) was administered to the culture medium of full-thickness human skin ex vivo for 24 or 72 h. RESULTS: Theophylline stimulated protein expression of the antioxidant metallothionein-1 and mRNA levels of collagen I and III. Assessment of fibrillin-1 immunohistology revealed enhanced structural stability of dermal microfibrils. Theophylline also exerted extracellular matrix-protective effects by decreasing MMP-2 and MMP-9 mRNA levels, partially antagonizing the effects of menadione, the potent, toxic ROS donor. In addition, it decreased menadione-stimulated epidermal keratinocytes apoptosis. Interestingly, theophylline also increased the level of intracutaneously produced melatonin, that is the most potent ROS-protective and DNA damage repair neuromediator, and tendentially increased protein expression of MT1, the melatonin receptor. Theophylline also increased the expression of keratin 15, the stem cell marker, in the epidermal basal layer but did not change mitochondrial activity or epidermal pigmentation. CONCLUSION: This ex vivo pilot study in human skin shows that theophylline possesses several interesting complex skin-protective properties. It encourages further examination of theophylline as a topical candidate for anti-ageing treatment. R esum e OBJECTIF: la th eophylline est un inhibiteur de la phosphodiest erase actuellement utilis ee en clinique pour le traitement de l'asthme. En outre, elle est reconnue comme etant un agent cosm etique ayant des propri et es potentiellement anti-âge et antioxydantes. Cependant, la mani ere dont elle affecte la peau chez l'homme est encore tr es peu etudi ee. M ETHODES: de la th eophylline (10 ou 100 lM) a et e ajout ee dans le milieu de culture d'un echantillon de peau humaine d' epaisseur totale ex vivo pendant 24 ou 72 h. Theophylline effects in human skin ex vivo M. Bertolini et al. 84 Theophylline effects in human skin ex vivo M. Bertolini et al. Theophylline effects in human skin ex vivo M. Bertolini et al. Theophylline effects in human skin ex vivo M. Bertolini et al.