Antioxidative system of Deinococcus radiodurans

Qi, Huizhou; Wang, Wu-zhou; He, Junyan; Ma, Yun; Xiao, Fangzhu; He, Shuya

doi:10.1016/j.resmic.2019.11.002

Cited by 44 publications

(41 citation statements)

References 96 publications

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“…In humans, the oxidative modification of cellular macromolecules by ROS is the basis of various degenerative diseases, cancer, and aging [5,6]. The extremophilic bacterium Deinococcus radiodurans is well known for its extreme resistance to various stresses including ionizing radiation (IR), oxidative stress, and desiccation [6,7]; however, the mechanisms responsible for the extreme resistance of this robust bacterium remain unclear. At high doses of IR, D. radiodurans can rapidly reassemble DNA fragments into complete chromosomes [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…At high doses of IR, D. radiodurans can rapidly reassemble DNA fragments into complete chromosomes [8,9]. Over the past few years, an effective DNA repair system was considered to be the decisive factor in ionizing radiation resistance of D. radiodurans [6]; however, compared to radiation-sensitive bacteria such as Escherichia Coli, no specific DNA damage repair protein has been identified in The susceptibility of D. radiodurans to H 2 O 2 was determined as in previous research [18]. Strains were cultured overnight in TGY broth at 30 • C and were grown in fresh TGY broth up to an optical density at 600 nm (OD 600 ) of 0.8.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Proteomics Analysis Reveals New Features of the Oxidative Stress Response in the Polyextremophilic Bacterium Deinococcus radiodurans

et al. 2020

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Deinococcus radiodurans is known for its extreme resistance to ionizing radiation, oxidative stress, and other DNA-damaging agents. The robustness of this bacterium primarily originates from its strong oxidative resistance mechanisms. Hundreds of genes have been demonstrated to contribute to oxidative resistance in D. radiodurans; however, the antioxidant mechanisms have not been fully characterized. In this study, comparative proteomics analysis of D. radiodurans grown under normal and oxidative stress conditions was conducted using label-free quantitative proteomics. The abundances of 852 of 1700 proteins were found to significantly differ between the two groups. These differential proteins are mainly associated with translation, DNA repair and recombination, response to stresses, transcription, and cell wall organization. Highly upregulated expression was observed for ribosomal proteins such as RplB, Rpsl, RpsR, DNA damage response proteins (DdrA, DdrB), DNA repair proteins (RecN, RecA), and transcriptional regulators (members of TetR, AsnC, and GntR families, DdrI). The functional analysis of proteins in response to oxidative stress is discussed in detail. This study reveals the global protein expression profile of D. radiodurans in response to oxidative stress and provides new insights into the regulatory mechanism of oxidative resistance in D. radiodurans.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative Proteomics Analysis Reveals New Features of the Oxidative Stress Response in the Polyextremophilic Bacterium Deinococcus radiodurans

et al. 2020

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“…Interestingly, many enzymatic antioxidants such as SOD and catalase require either Fe or Mn for their activity. Alterations in total intracellular Mn levels can alter ROS sensitivity in bacteria and high intracellular Mn is one of the contributing factors for unusually high oxidative stress tolerance of radioresistant Deinococcus radiodurans [47, 49]. Further, the coordinated decrease in Mn quota, can also be the adaptive response to reduce the transport of electrons through PSII.…”

Section: Discussionmentioning

confidence: 99%

Iron acquisition across outer membrane: The role of major outer membrane protein Slr1908 under iron supplemented conditions inSynechocystis6803

Agarwal

Choudhury

Akkipeddi

2021

Preprint

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The Ton-B dependent outer membrane (OM) transporters are responsible for active iron (Fe) import in Synechocystis sp. strain PCC 6803 (S. 6803 or WT) under Fe depletion. However, the mechanism of Fe acquisition under Fe supplemented conditions remains uncharacterised. In the present study, functional role of OMP Slr1908 in S. 6803 was addressed by insertional mutagenesis. The Δslr1908 cells exhibited slower growth in the first week in comparison to the WT and displayed an absorption and 77K fluorescence spectrum typical of Fe deficiency. Indeed, the mutant had ∼ 80% less Fe as confirmed by atomic absorption spectroscopy and 55Fe-radiotracer uptake. The iron deficiency was paralleled with low Mn content. The mutant had low SOD content as well as activity, less cytochromes, less chlorophyll content, less Fv/ Fm, lower ETRII and high oxidative stress in comparison to the WT at the end of first week. Interestingly, the mutant showed transcriptional upregulation of iron stress induced protein isiA and isiB signifying intracellular Fe deficiency. Upregulation of OMP Slr0042 was also observed at RNA and protein level. The results indicate that Slr1908 is a major Fe uptake OMP in S. 6803 the deletion of which leads to initial slow growth that gets partially offset by induction of other Fe importing OMPs.

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“…For example, D. radiodurans R1, the most extensively studied Deinococcus , tolerates up to 5000 Gy of acute ionizing radiation with no loss of viability, which is 3000 times that of human cells and 250 times that of Escherichia coli [5] . It has been proposed that both the sophisticated DNA repair mechanism and antioxidation system enhance the robustness of D. radiodurans [1] , [6] , [7] , [8] .…”

Section: Introductionmentioning

confidence: 99%

“…Unique proteins involved in the extreme resistance have been characterized by genetic, biochemical and biophysical analyses, with their structures recently reported in literatures. Previous reviews have investigated some of these proteins as well as the mechanisms of radiation and oxidative stress resistance in D. radiodurans [1] , [6] , [8] , [37] , which will not be discussed in this review. Here, we focus on the unique and general structural properties of these stress-related proteins identified over the past few years ( Table 1 and Fig.…”

Section: Introductionmentioning

confidence: 99%

Structural features and functional implications of proteins enabling the robustness of Deinococcus radiodurans

Chen

Tang

Hua

et al. 2020

Computational and Structural Biotechnology Journal

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Deinococcus radiodurans can survive under extreme conditions, including high doses of DNA damaging agents and ionizing radiation, desiccation, and oxidative stress. Both the efficient cellular DNA repair machinery and antioxidation systems contribute to the extreme resistance of this bacterium, making it an ideal organism for studying the cellular mechanisms of environmental adaptation. The number of stress-related proteins identified in this bacterium has mushroomed in the past two decades. The newly identified proteins reveal both commonalities and diversity of structure, mechanism, and function, which impact a wide range of cellular functions. Here, we review the unique and general structural features of these proteins and discuss how these studies improve our understanding of the environmental stress adaptation mechanisms of D. radiodurans .

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Antioxidative system of Deinococcus radiodurans

Cited by 44 publications

References 96 publications

Comparative Proteomics Analysis Reveals New Features of the Oxidative Stress Response in the Polyextremophilic Bacterium Deinococcus radiodurans

Comparative Proteomics Analysis Reveals New Features of the Oxidative Stress Response in the Polyextremophilic Bacterium Deinococcus radiodurans

Iron acquisition across outer membrane: The role of major outer membrane protein Slr1908 under iron supplemented conditions inSynechocystis6803

Structural features and functional implications of proteins enabling the robustness of Deinococcus radiodurans

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