2006
DOI: 10.1016/j.str.2005.10.010
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Antiparallel Four-Stranded Coiled Coil Specified by a 3-3-1 Hydrophobic Heptad Repeat

Abstract: Coiled-coil sequences in proteins commonly share a seven-amino acid repeat with nonpolar side chains at the first (a) and fourth (d) positions. We investigate here the role of a 3-3-1 hydrophobic repeat containing nonpolar amino acids at the a, d, and g positions in determining the structures of coiled coils using mutants of the GCN4 leucine zipper dimerization domain. When three charged residues at the g positions in the parental sequence are replaced by nonpolar alanine or valine side chains, stable four-hel… Show more

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Cited by 60 publications
(80 citation statements)
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“…S1b), consistent with a well-folded complex having an extensive hydrophobic core. The melting temperature (apparent Tm = 40.6, 40.8 °C (n = 2)) was lower than those of other naturally occurring coiled-coil proteins (Tm > 60 °C) [26][27][28][29] , and unfolding of the mHv1/VSOP-cc coiled-coil was irreversible (Fig. 3d).…”
Section: Coiled-coil Assembly Domain Of Mhv1/vsop and Its Structurementioning
confidence: 85%
“…S1b), consistent with a well-folded complex having an extensive hydrophobic core. The melting temperature (apparent Tm = 40.6, 40.8 °C (n = 2)) was lower than those of other naturally occurring coiled-coil proteins (Tm > 60 °C) [26][27][28][29] , and unfolding of the mHv1/VSOP-cc coiled-coil was irreversible (Fig. 3d).…”
Section: Coiled-coil Assembly Domain Of Mhv1/vsop and Its Structurementioning
confidence: 85%
“…a') in this figure and throughout the main text is used to indicate a residue on the opposite chain. B An antiparallel tetramer with a 3-3-1 repeat, as in Figure 2B and reference [14]. C A parallel seven-helix coiled coil with a 3-1-2-1 hydrophobic pattern, as in Figure 2D and reference [19].…”
Section: Discussionmentioning
confidence: 99%
“…A When all e positions are mutated to Val, departing from the canonical 3-4 repeat and creating a 3-3-1 hydrophobic repeat, the resulting sequence gives a parallel tetramer [15]. B When all g positions are substituted with either Val or Ala, also producing a 3-3-1 repeat, the result is an anti-parallel tetramer [14]. C A right-handed parallel coiled-coil tetramer [18].…”
Section: Discussionmentioning
confidence: 99%
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“…The interdigitated helical backbones are offset by ϳ0.25 of a heptad repead, which, along with the short ϳ8 Å distance between the helical axes, is a characteristic of a ferritin-type Alacoil (11). Alacoils have been previously observed in designed protein sequences (12)(13)(14)(15). There are no interchain salt bridges between any of the peptides in the octamers (Fig.…”
Section: Resultsmentioning
confidence: 88%