Antiparasitic efficacy of Gracillin and Zingibernsis newsaponin from <i>Costus speciosus</i> (Koen ex. Retz) Sm. against <i>Ichthyophthirius multifiliis</i>

Wang, Zheng; Yan, Chunhong; Zhang, Yabin; Li, Zehong; Li, Zhongqiang; Li, Xiuying; Wang, Zhang-Wei; Wang, Xiulan; Chen, Weiqiang; Yu, Xinhai

doi:10.1017/s0031182014001358

Cited by 21 publications

(13 citation statements)

References 22 publications

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“…These bioactive properties of the essential oil of L. alba are attributed to the richness of its major chemical constituents, such as geranial, carvone, neral, linalool and limonene. Recently, use of natural products derived from plants, for controlling parasites, has received great attention (Boijink et al, 2015;Hashimoto et al, 2016;Huang et al, 2013;Ji et al, 2012;Steverding et al, 2005;Xiao-Feng et al, 2014;Zhang et al, 2013;Zhang et al, 2014;Zheng et al, 2015). However, the effects of the essential oil of L. alba on ectoparasites of fish have not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects

Soares

Neves²,

Oliveira

et al. 2016

Aquaculture

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This study investigated the in vivo and in vitro antiparasitic effects of the essential oil of Lippia alba and the bloodrelated and histopathological alterations that it causes in Colossoma macropomum. In the in vitro trial, the anthelminthic effects of 160, 320, 640, 1280 and 2560 mg/L of the essential oil were tested against monogenoideans (Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri) of the gills of this fish, which are its natural parasites. The concentrations of 1280 mg/L and 2560 mg/L showed 100% efficacy after 20 min of exposure to the essential oil, while at lower concentrations this efficacy against the gill monogenoideans only occurred after 2-3 h of in vitro exposure. However, in the controls, mortality of all of these monogenoideans only occurred after 9 h. A total of 240 fry were distributed into four treatments (20 fish per repetition) and three repetitions were used in the in vivo trial for baths with 100 and 150 mg/L of the essential oil of L. alba, for 30 min. The efficacy in this trial against Ichthyophthirius multifiliis in fish exposed to 100 and 150 mg/L of the essential oil was 40.7% and 50.3%, respectively. However, for monogenoideans, there was efficacy of 14.0% only in the fish exposed to 100 mg/L of the essential oil used. Moreover, the fish exposed to these concentrations of the essential oil presented increased plasma glucose levels, thus denoting signs of stress. Severe lesions such as hyperplasia, fusion of the lamellar epithelium, capillary dilatation, epithelial detachment, lamellar aneurysm, epithelial rupture with hemorrhage, congestion, edema, necrosis, mucous cell proliferation, chloride cells and lamellar hypertrophy were observed in the gills of the fish exposed to 100 and 150 mg/L of the essential oil of L. alba. Alterations to total protein levels, hemoglobin, hematocrit, red blood cell number, thrombocytes number, white blood cell number, lymphocytes, eosinophils and blood neutrophil number was also observed in these fish. The essential oil of L. alba showed great potential for antiparasitic treatment, given that it had high in vitro efficacy against monogenoideans and in vivo efficacy against the protozoon I. multifiliis. Because of the low concentrations of the essential oil (100 and 150 mg/L) that were tolerated by the fish and thus could be used in the therapeutic baths, the efficacy against monogenoideans was low. This indicates that there is a need for additional strategies for using this essential oil in antiparasitic treatments, since the concentrations that eliminate these ectoparasites are toxic for the hosts. Lastly, this was the first study on the antiparasitic activity of L. alba. Statement of Relevance The manuscript entitled "Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects", represents original article on use of the essential oil of Lippia alba on ectoparasites of tambaqui, an important finfish of Amazon regio...

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Section: Introductionmentioning

confidence: 99%

Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects

Soares

Neves²,

Oliveira

et al. 2016

Aquaculture

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“…The acute toxicity data showed that the 96 hr‐LC 50 value of TA to zebrafish was 19.51 ppm. This amount was more than the 96 hr‐LC 50 values obtained for potassium permanganate (17.6 mg/L) to channel catfish fingerlings (Tucker, ), malachite green (1.0 mg/L) to catfish (Srivastava, Singh, Srivastava, & Sinha, ), copper sulphate (6.89 mg/L) to channel catfish (Straus, ), dihydrochelerythrine (18.2 mg/L) to richadsin ( Squaliobarbus curriculus ) (Yao et al., ), dihydrosanguinarine (13.3 mg/L) and chelerythrine (3.3 mg/L) to goldfish (Shan et al., ), psoralidin (5.6 mg/L) to goldfish (Song et al., ) and gracillin (1.64 mg/L) to grass carp (Zheng et al., ). There is a low toxicity for TA to fish; therefore, this phenolic compound is a safe agent to control ichthyophthiriasis infection on zebrafish in the aquaculture industry.…”

Section: Discussionmentioning

confidence: 75%

The in vitro and in vivo effect of tannic acid on Ichthyophthirius multifiliis in zebrafish (Danio rerio) to treat ichthyophthiriasis

Alavinia

Mirzargar

Rahmati‐Holasoo

et al. 2018

Journal of Fish Diseases

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The in vitro antiparasitic effect of polyphenol tannic acid (TA) on Ichthyophthirius multifiliis theronts and tomonts was evaluated. In vitro antiparasitic assays revealed that TA in a dose- and time-dependent pattern through the damage of parasite plasma membrane could be 100% effective against I. multifiliis theronts at concentrations of 8 and 11 ppm during all the exposure times (45-270 min). The tomonts proliferation was completely inhibited by penetrating TA (at least 15 ppm for 22-hr exposure) into encysted tomont across the cyst wall. However, 10 ppm TA could result in a ninefold decrease in the population of live tomonts compared to the control group (p < 0.05). Although at theront concentrations of over 6,000 per zebrafish (Danio rerio), a 100% prevalence of ichthyophthiriasis during a 5-day exposure was recorded, results of in vivo tests showed that the parasite that pretreated up to 10 ppm TA for 70 min had not any capability to infect the studied zebrafish population. The acute toxicity (96 hr-LC ) of TA for zebrafish was 19.51 ppm. Thus, TA can be considered as a natural therapeutant to safely and efficiently improve the health of aquatic systems by controlling ichthyophthiriasis.

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“…3 ) from D. tokoro , whose structures were closely related to PD. Dioscin is reported to have platelet-aggregation inhibitory [6], antifungal [8], and hypoglycemic [9] activities, and gracillin was reported as an antiparacitic agent [10]. Interestingly, D. tokoro rhizome extracts exhibited different compound contents depending on the locations of the plant collected.…”

Section: Resultsmentioning

confidence: 99%

Protodioscin, Isolated from the Rhizome of Dioscorea tokoro Collected in Northern Japan is the Major Antiproliferative Compound to HL-60 Leukemic Cells

Oyama

Tokiwano

Kawaii

et al. 2017

CBC

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Background: The rhizome of Oni-dokoro (a wild yam, Dioscorea tokoro) has extremely bitter taste and is not generally regarded edible;, however, in northern part of Japan, such as Iwate and a part of Aomori, it is used as health promoting food. To clarify the reason, we examined the biologically active compounds in the rhizome collected at Iwate and compared them from the other area in literature.Methods: The acetonitrile extract from northern part of Japan was purified by bioassay-guided separation using antiproliferative activity to human leukemia HL-60 cell, and protodioscin (PD) was isolated and identified by instrumental analyses as the major active compound.Results: PD known as a saponin with four sugar moieties, an inhibitor for platelet aggregation, and a low density lipoprotein (LPL) lowering agent, displayed strong growth inhibitory effect to HL-60. The literature search suggested that the rhizome from other area contained dioscin and other saponins with three sugar moieties as their major component. We assume that the edible and health promoting effect of the rhizome in the particular area is partially derived from these different components.Conclusion: We were interested in the differences of utilization in the rhizome of wild yam Dioscorea tokoro, and examined the chemical composition in the rhizome to find protodioscin as antiproliferative compound to HL-60. In the report from other area, the rhizome exhibited dioscin as the major compound. Our study indicated that the protodioscin/dioscin composition varied regionally, although the reason is still needs to be investigated.

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Antiparasitic efficacy of Gracillin and Zingibernsis newsaponin from Costus speciosus (Koen ex. Retz) Sm. against Ichthyophthirius multifiliis

Cited by 21 publications

References 22 publications

Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects

Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects

The in vitro and in vivo effect of tannic acid on Ichthyophthirius multifiliis in zebrafish (Danio rerio) to treat ichthyophthiriasis

Protodioscin, Isolated from the Rhizome of Dioscorea tokoro Collected in Northern Japan is the Major Antiproliferative Compound to HL-60 Leukemic Cells

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