Antiperlecan Antibodies Are Novel Accelerators of Immune-Mediated Vascular Injury

Abstract

Acute vascular rejection (AVR) is characterized by

Heterogeneity of chronic graft-versus-host disease biomarkers: association with CXCL10 and CXCR3+ NK cells

“…In addition, its highest predictive value was increased in combination with other biomarkers, but they were not identical in the 2 replication sets. Its association was highest in replication set 1 with anti-LG3, a vascular rejection biomarker in kidney transplantation, 12,13 and in replication set 2, with CXCL9 and CXCR3 1 regulatory NK (NK reg ) cells.…”

“…12 Anti-LG3 titers were measured with previously described laboratory-made enzyme-linked immunosorbent assay (ELISA). 13 Briefly, recombinant mouse LG-3 (10 mg/mL) was first coated on 96-well Nunc MaxiSorp plates (Thermo Scientific, Rochester, NY) for a total of 1 mg per well.…”

Pre-Transplant IgG Reactivity to Apoptotic Cells Correlates With Late Kidney Allograft Loss.

“…Additional studies support a contribution of non-HLA antibodies to pre-sensitization (7,13). More specifically, serum IgG reactivity to autoantigens such as cardiac myosin, vimentin, collagen, oxidized lipids and LG3 has been associated with increased rejection rates and reduced graft survival (14)(15)(16)(17)(18)(19)(20)(21)(22)(23).…”

The PROCARE consortium: Toward an improved allocation strategy for kidney allografts

“…As graft loss in this study could not be the result of anti-HLA alloreactivity, these data suggest an important role for immunity against antigens other than HLA in kidney transplantation. A substantial number of non-HLA antigens have been identified as targets of autoantibodies in kidney transplantation including vimentin, tubulin, myosin, collagen V, angiotensin type 1 receptor (AT1r), agrin, and LG3 [12][13][14][15][16][17][18][19]. The presence of antibodies against the glomerular basement membrane protein agrin was found in 11 of 16 patients with chronic transplant glomerulopathy and 3 of 6 controls with chronic allograft nephropathy in absence of chronic transplant glomerulopathy.…”

“…In addition, in multiple studies autoantibodies against non-HLA antigens have been found in sera from patients on the waiting list for kidney transplantation. Depending on their specificity the presence of these antibodies has been associated with chronic transplant glomerulopathy, a higher number of acute rejection episodes, acute vascular rejection, refractory acute vascular rejection and malignant hypertension [10][11][12][13][14][15][16][17][18][19]. These data indicate that detection of preformed non-HLA antibodies may be useful in identifying patients at risk for graft loss.…”