Antiplasmodial activity and phytochemical analysis of extracts from selected Ugandan medicinal plants

Adia, Madina Mohamed; Emami, S. Noushin; Boyer, Robert; Faye, Ingrid; Borg‐Karlson, Anna‐Karin

doi:10.1016/j.jep.2016.03.047

Cited by 44 publications

(23 citation statements)

References 31 publications

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“…We found all of these to be inactive. Trans-fagaramide has been reported to have relatively weak activity against the chloroquine-sensitive strain NF54, but much better activity against the chloroquine-resistant strain FCR 3 [7]. In agreement with this, we found that trans-fagaramide was inactive against the chloroquine-sensitive strain 3D7 at the highest concentration tested (20 µg/mL).…”

Section: Resultssupporting

confidence: 88%

“…Commonly reported indications are pain, rheumatism, dysentery, fever and sickle-cell anemia. Several Zanthoxylum species, e.g., Zanthoxylum gillettii [6], Zanthoxylum chalybeum [7], and Zanthoxylum usambarense [8], have been reported to be used against malaria or fever, as has Z. zanthoxyloides [9]. In Mali, a maceration of the roots is used [10], in Guinea and Ghana, a decoction of the stem bark (SB) is used [11,12], and in Burkina Faso, the SB is used [13].…”

Section: Introductionmentioning

confidence: 99%

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Anti-plasmodial Effects of Zanthoxylum zanthoxyloides

et al. 2019

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Zanthoxylum zanthoxyloides, syn. Fagara zanthoxyloides, is a tree growing in West Africa and is used in traditional medicine against a variety of diseases, including malaria. In the work reported here, root bark and stem bark extracts of this tree, as well as compounds isolated from the extracts, have been investigated for activity in vitro against chloroquine-sensitive and chloroquine-resistant strains of Plasmodium falciparum. In addition, toxicity against nauplii of the brine shrimp Artemia salina has been studied. Dichloromethane extracts of the root bark and stem bark, and a methanol extract of the stem bark, showed anti-parasitic activity towards chloroquine-sensitive as well as chloroquine-resistant P. falciparum, with IC50 values between 1 and 10 µg/mL. Among the isolated compounds, bis-dihydrochelerythrinyl ether, buesgenine, chelerythrine, γ-fagarine, skimmianine, and pellitorine were the most active, with IC50 values of less than 5 µg/mL. The dichloromethane extracts were toxic to brine shrimp nauplii, with LC50 values of less than 1 µg/mL. Methanol extracts were much less toxic (LC50 between 50 and 100 µg/mL). Among the isolated substances, bis-dihydrochelethrinyl ether was the most toxic (LC50 ca. 2 µg/mL).

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Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Anti-plasmodial Effects of Zanthoxylum zanthoxyloides

et al. 2019

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“…By comparing our results with those of the classification, the antiplasmodial activity of EtOH-H2O extracts of Acacia gourmaensis, were found to be moderate (19.9%) of inhibition dosed of 150mg/kg. Several studies showed that this antiplasmodial activity is correlated with secondary metabolites contents such as polyphenols and flavonoids (Khalid et al, 1986;Cubukcu et al, 1990;Tahir et al, 1999;Bagavan et al, 2011;Adia et al, 2016). In 1999, Tahir and collaborators had accused terpenoids and tannins in the best antiplasmodial activities observed against P. falciparum of the extracts of A. nilotica.…”

Section: In Vivo Antiplasmodial Activitymentioning

confidence: 99%

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2019

IBSPR

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In this present study, in vivo antiplasmodial and antioxidant activities of Acacia gourmaensis A. RICH. (Mimosaceae) were evaluated based on its total extracts (ethanol-water, decoction, acetone), fractions (hexane fraction, ethyl acetate fraction, butanol fraction and aqueous fraction) and mineral contents. Total extract of decoction, acetone and EtOH-H2O fractions were used to evaluate chemical composition using HPLC-GC/MS-DAD methods. This plant is used in traditional medicine against liver disease, fractures, coughing fits and especially paludism. The antioxidant property was analyzed using 3 methods such as total antioxidant activity (TAC), iron reducing power (IRP) and radical scavenging activity (DPPH*). Antiplasmodial activity was performed in mice using red blood infected by Plasmodium berghei. The biological activities (antioxidants and antiplasmodial) were directed against plant phytochemical content 9 polyphenolic compounds were highlighted in the HPLC-DAD method, against 3 in HPLC-GC/MS. EtOH-H2O gave the best anti-TAC with 6.53 ± 0.15 mgEAA/mL. However, this extract (EtOH-H2O) gave the weakest activity with radical scavenging activity (23.73 ± 0.11 µg/mL). The best anti-DPPH* is obtained with acetone extract (1.93 ± 0.05 µg/mL). Concerning anti-IRP activity, hexane fraction gave the best result with 0.12 ± 0.00 µg/mL. At the antiplasmodial level, the best percentage reduction (19.9 %) is obtained with a dose of 150 mg/kg. On the phytochemistry plan, aqueous and ethyl acetate fractions given the best content with respectively 119.14 ± 8.13 mg EAG/g for total phenolic and 5.33 ± 0.17 EQ/g for total flavonoids. Mineral content quantification show that this plant is rich in calcium with 23.43 ± 1.32 mmol/L. The results due to the antiplasmodial activity could justify the traditional use of this plant.

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“…Leaves and roots of Clerodendrum rotundifolium have been used during induction of labour in childbirth and in treatment of diabetes, stomachache, malaria, microbial infections and intestinal parasites [22].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity, Total Phenols, Flavonoids and LCMS Profile of Chamaecrista hildebrandtii (Vatke) Lock and Clerodendrum rotundifolium (Oliv.)

Odhiambo

Kareru

Mwangi

et al. 2019

EJMP

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Aims: The purpose of the study was to determine the antioxidant activity, quantify total phenols and total flavonoids and characterize the secondary metabolites present in methanolic extracts of Chamaecrista hildebrandtii and Clerodendrum rotundifolium using liquid chromatography coupled to mass spectrometry (LC-MS). Methodology: The total phenol and flavonoid contents were determined spectrophotometrically while the antioxidant activity was evaluated using the 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) free radical scavenging method. The secondary metabolites present in the methanolic leaves extracts were evaluated using LC-MS. Results: The extracts of C. hildebrandtii showed a significantly higher antioxidant activity (IC50 = 8.7 mg/mL) compared to C. rotundifolium (IC50= 28.5 mg/mL). Both methanolic extracts of C. hildebrandtii and C. rotundifolium had common and different types of flavonoids such as quercetin, rutin, (+)-catechin 3-O-gallate and luteolin 6-C-glucoside among others that could be responsible for the observed antioxidant activity. The total phenolic content of C. hildebrandtii (1.33±0.07 mg/g tannic acid equivalents) was significantly higher than that of C. rotundifolium (0.25±0.00 mg/g tannic acid equivalents). However, there was no statistically significant difference (p>0.05) in total flavonoid content of C. hildebrandtii (2.69±0.33 mg/g catechin equivalents) and C. rotundifolium (2.36±0.16 mg/g catechin equivalents). Conclusion: The results of the present study suggested that the good antioxidant activity exhibited by C. hildebrandtii may probably have been brought about by various secondary metabolites functioning in synergy.

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Antiplasmodial activity and phytochemical analysis of extracts from selected Ugandan medicinal plants

Cited by 44 publications

References 31 publications

Anti-plasmodial Effects of Zanthoxylum zanthoxyloides

Anti-plasmodial Effects of Zanthoxylum zanthoxyloides

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Antioxidant Activity, Total Phenols, Flavonoids and LCMS Profile of Chamaecrista hildebrandtii (Vatke) Lock and Clerodendrum rotundifolium (Oliv.)

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