Antiplasmodial Activity of Extracts and Alkaloids of Three Alstonia Species from Thailand

Keawpradub, Niwat; Kirby, G. C.; Steele, Jonathan; Houghton, Peter J.

doi:10.1055/s-1999-14043

Cited by 137 publications

(114 citation statements)

References 0 publications

Supporting

Mentioning

111

Contrasting

Unclassified

Order By: Relevance

“…Differences in the biology of CQR P. falciparum strains and the CQS P. berghei strain used in that study were considered to explain the negative in vivo result, since it had been shown that this alkaloid was essentially active against CQR strains of P. falciparum, and a CQS P. berghei strain was used in that study (Frederich et al 1999). villalstonine and macrocarpamine were the most active against the CQR K1 strain in vitro (IC 50 270 and 360 nM, respectively) (Wright et al 1993, Keawpradub et al 1999. Voacamine, isolated from Tabenamontana fuchsiaefolia A. DC.…”

Section: Alkaloids: New Leads For Antimalarial Drugs?mentioning

confidence: 99%

Plant-derived antimalarial agents: new leads and efficient phythomedicines. Part I. Alkaloids

Oliveira

Dolabela

Braga

et al. 2009

An. Acad. Bras. Ciênc.

128

View full text Add to dashboard Cite

Malaria remains one of the most serious world health problem and the major cause of mortality and morbidity in the endemic regions. Brazil is among the 30 high-burden countries and most of the cases occur in the Legal Amazonian Region. New chemotherapeutical agents are needed for the treatment of malaria. Many plant species are used in traditional medicines of malarious countries and a relatively few number of these have been investigated for evaluation of their antimalarial effect. Still lower is the number of those that have had the active natural compounds isolated and the toxicity determined. This area is, then, of great research interest. A discovery project of antimalarial natural products from plants traditionally used to treat malaria must include in vitro and in vivo assays as well as bioguided isolation of active compounds. The final products would be antimalarial chemical entities, potential new drugs or templates for new drugs development, and/or standardized antimalarial extracts which are required for pre-clinical and clinical studies when the aim is the development of effective and safe phythomedicines. This review discusses these two approaches, presents briefly the screening methodologies for evaluation of antimalarial activity and focuses the activity of alkaloids belonging to different structural classes as well as its importance as new antimalarial drugs or leads and chemical markers for phytomedicines.

show abstract

Section: Alkaloids: New Leads For Antimalarial Drugs?mentioning

confidence: 99%

Plant-derived antimalarial agents: new leads and efficient phythomedicines. Part I. Alkaloids

Oliveira

Dolabela

Braga

et al. 2009

An. Acad. Bras. Ciênc.

128

View full text Add to dashboard Cite

show abstract

“…Substituted indoles have been referred to as "privileged structures" since they are capable of binding to many receptors with high affinity [1]. Cycloocta [b]indoles, an emerging class of biologically relevant indole analogues that has been found in many naturally occurring alkaloids such as macroline, ajmaline, macrocarpamine, villalstonine, O-acetyl macralstonine and macralstonine ( Figure 1) and known to exhibit wide range of pharmacological properties such as antiamoebic, antiplasmodic, antiprotozol and antihypertensive activities [2][3][4][5]. The cycloocta [b] indole framework has recently been a subject of biology-oriented synthesis (BIOS) [6] and their analogues are promising targets for developing a novel class of potent and selective Mycobacterium protein tyrosine phosphatase B (MptpB) inhibitors against Mycobacterium tuberculosis [7].…”

Section: Introductionmentioning

confidence: 99%

An Expedient General Synthesis of Quinolino and Pyrrolocycloocta[b] Indoles

Vairavelu¹,

Kj²

2016

Organic Chem Curr Res

View full text Add to dashboard Cite

“…5 The other medicinal plants that have been used as antimalarial agents in Indonesia are Carica papaya, 6 Phyllantus niruri, 7 and Alstonea scolaris. 8 C. bicolor PF Stevens or Bintagor is one of the species of genus Calophyllum (Clusiaceae family) that traditionally used as a treatment of various diseases. 9 Calophyllum has 180-200 species that grow in the coastal areas in Kalimantan and Papua.…”

Section: Introductionmentioning

confidence: 99%

ANTI-PLASMODIUM FALCIPARUM IN VITRO ACTIVITY OF CALOPHYLLUM BICOLOR EXTRACT: Morphology and Ultra Structure

et al. 2015

View full text Add to dashboard Cite

Background: Anti-malarial resistance of Plasmodium, such as chloroquine becoming a health problem worldwide. This research aims to evaluate the in vitro anti-plasmodium activity of hexane fraction extract of Calophyllum bicolor (C. bicolor) against culture of Plasmodium falciparum (P. falciparum) 3D7 and determine its inhibitor concentration of 50% (IC 50 ); to observe the microscopic changes of P. falciparum after exposed to the hexane fraction of C. bicolor extract and to observe the ultra structure changes of P. falciparum 3D7 after being exposed to hexane fraction of C. bicolor extract. Method: This research applied quasi experimental with post-test only control group design. Anti malarial activity test of hexane fraction of C. bicolor extract towards Plasmodium falciparum 3D7 2% was undertaken over 48 hours with in vitro incubation technique. The level of parasitemia was observed using binocular optical microscope with 1000x magnification by counting the infected erythrocytes with Giemsa color technique. Then it was analyzed to determine the level of inhibitor concentration of 50% (IC 50 ) of the C. bicolor extract. The next step is to observe the changes of parasite's morphologic and ultra structure after the 24 and 48 hours incubation of the parasite P. falciparum 3D7 with hexane fraction of C. bicolor, IC 50 . Then, the morphologic change of P. falciparum 3D7 was observed with optical binocular microscopy and the ultra structure changes with Transmission Electron Microscopy (TEM). The changes of morphologic and ultra structure were analyzed qualitatively. Results: The research revealed that hexane fraction of the C. bicolor extract inhibited the growth of the parasite P. falciparum 3D7 with the value of -49.00±2.54, 12.53±3.13, 23.01±1.10, 27.68±4.23, 48.65±18.71, 70.82±4.67, 80.52±6.17% with the hexane extract concentration of 0, 0.001, 0.01, 0.1, 1, 10, 100, and 1000 µg/mL. This research also found that the inhibitor concentration of 50% (IC 50 ) of hexane fraction of C. bicolor extract was 3.94 µg/mL in 10 -7 which could change the morphologic and ultra structure of P. falciparum 3D7. There were several changes in morphology of the parasite over 24 hour incubation compared with the control group, i.e. the nucleus of young tropozoid became thicker, darker, and smaller; the skizon were condensate, thicker, darker, karyorrhexis and demolition of membrane. Hexane fraction of C. bicolor extract also changes the ultra structure over 24 hour incubation, i.e. vacuole membrane boundary and hemozoin were unclear. Over 48 hours of incubation the nucleus and cytoplasm bigger than those in the control group. Conclusion: Hexane fraction of the C. bicolor extract showed a strong anti plasmodium activity towards the in vitro culture of P. falciparum 3D7 at the level of (IC 50 ) 3.94 µg/mL. The Hexane fraction of C. bicolor extract could change the morphology and ultra structure of P. falciparum 3D7 in vitro.

show abstract

Antiplasmodial Activity of Extracts and Alkaloids of Three Alstonia Species from Thailand

Cited by 137 publications

References 0 publications

Plant-derived antimalarial agents: new leads and efficient phythomedicines. Part I. Alkaloids

Plant-derived antimalarial agents: new leads and efficient phythomedicines. Part I. Alkaloids

An Expedient General Synthesis of Quinolino and Pyrrolocycloocta[b] Indoles

ANTI-PLASMODIUM FALCIPARUM IN VITRO ACTIVITY OF CALOPHYLLUM BICOLOR EXTRACT: Morphology and Ultra Structure

Contact Info

Product

Resources

About