Antiplasmodial activity of Indigofera spicata root extract against Plasmodium berghei infection in mice

Birru, Eshetie Melese; Geta, Mestayet; Gurmu, Abyot Endale

doi:10.1186/s12936-017-1853-5

Cited by 31 publications

(37 citation statements)

References 28 publications

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“…The dose-dependent reduction in parasitemia levels produced by the methanol aerial extract of Alysicarpus glumaceus (average parasitemia 20.46 ± 1.31, 15.48 ± 1.37 and 12.26 ± 0.79 at 250, 500, and 1,000 mg/kg, respectively), is an indication of the extracts ability to inhibit the proliferation of Plasmodiumparasite in mice (Figure 3). The current finding agreed with other reports on medicinal plants used for malaria, such as Indigofera spicata, 8 Strycnos mitis 21 and Trema orientalis. 23 The oral median lethal dose was estimated to be greater than 5,000 mg/kg as there was no mortality recorded after the first and second phases of the acute toxicity study.…”

Section: Resultssupporting

confidence: 93%

“…7 These plants have served as sources for new drugs including some of the most successful antimalarial agents, such as quinine and artemisinin. 8 Alysicarpus glumaceus (Family-Fabaceae) is a shrubby annual plantwhich can grow up to 1 m high. The plant is found in the grassy savanna of Upper Volta, Northern Ghana and Northern Nigeria, and is also widely dispersed over the rest of tropical Africa.…”

Section: Introductionmentioning

confidence: 99%

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Antimalarial Activity of the Methanol Aerial Extract of Alysicarpus glumaceus (Vahl) DC. in Plasmodium berghei Infected Mice

Olorukooba¹,

Khan²,

Madu³

2019

TJNPR

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Article history:Medicinal plants are a potential source of affordable and effective drugs used in the treatment of many diseases. The plant Alysicarpus glumaceus has been used in traditional medicine for the treatment of many ailments including; thrush, sore, asthma, burn, and fever. The present study was designed to investigate the antimalarial activity of the methanol aerial extract of Alysicarpus glumaceus in Plasmodium berghei infected mice using suppressive, curative and prophylactic models. Phytochemical screening using standard procedures and acute toxicity studies via the oral route in mice were also conducted. Results from the phytochemical screening revealed the presence of glycosides, saponins, tannins, flavonoids, triterpenes, and alkaloids. The oral mean lethal dose (LD50) was estimated to be greater than 5,000 mg/kg in mice. The methanol aerial extract of Alysicarpus glumaceus at doses of 250, 500 and 1,000 mg/kg produced a significant (p<0.05) dose-dependentchemosuppression in the suppressive, curative and prophylactic tests respectively. There was a statistically significantly (p<0.05) prolongation of mean survival time in the extract treated mice in the curative study. The results of this investigation suggest that the methanol aerial extract of Alysicarpus glumaceus contains bioactive constituents with antimalarial activity.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Antimalarial Activity of the Methanol Aerial Extract of Alysicarpus glumaceus (Vahl) DC. in Plasmodium berghei Infected Mice

Olorukooba¹,

Khan²,

Madu³

2019

TJNPR

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“…However, non-significant difference was observed across all groups except Group 2 mice that were passaged but untreated. This result is consistent with (Birru et al, 2017), in which neither the extract doses nor the standard drug-treated group demonstrated statistically significant difference in body weight compared to the control group. The discrepancy was attributed to imbalance of the extract to potentiate protective effect and the cumulative pathophysiologic changes associated with the infection (Birru et al, 2017).…”

Section: Discussionsupporting

confidence: 90%

“…The percentage of red blood cells (RBCs) infected with malaria parasites were determined microscopically using the x100 objective with immersion oil in 10 different fields on each slide. The % Parasitemia and % Suppression were calculated using the formula (Birru et al, 2017):…”

Section: Measurement Of Parasitaemiamentioning

confidence: 99%

Malaria parasite clearance rate of crude methanol extract of Cryptolepis sanguinolenta in mice infected with chloroquine sensitive strain of Plasmodium berghei

Eze¹,

Attama²,

Ibezim³

et al. 2018

J. Med. Plants Res.

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This study aims to determine the malaria parasite clearance rate of crude methanol extract of Cryptolepis sanguinolenta in mice infected with chloroquine sensitive strain of Plasmodium berghei. P. berghei was injected in mice and left for 3 days for establishment. Blood sample collected and diluted with phosphate buffer saline was used for infection. Five (5) groups of animals (mice) were used in this study each containing 5 animals each. The body weights of the entire animal were recorded before and after treatment. Group 1 (normal control), Group 2 (positive control, untreated malaria-passaged mice), Group 3 (standard control, malaria -passaged mice treated with 25 mg/kg body weight of chloroquine), Group 4 (malaria-passaged mice treated with 200 mg/kg body weight of extract), and Group 5 (malariapassaged mice treated with 400 mg/kg body weight of extract). Hematological assessments were carried out before the experiment, 5 days after infection and after treatment. The percentage of parasite load in malaria passaged mice was found to be significantly (p < 0.05) lower in animals treated with mid and high doses of the extract when compared to control groups. Before treatment, no significant (p > 0.05) elevation was observed in the body weight of mice. On day 5 after infection, dose-dependent significant (p < 0.05) decrease was observed in the test groups. After treatment period, the body weights of the animals exhibited dose-dependent increase. The study thus revealed that Cryptolepis sanguinolenta root extracts possesses antimalarial activity in the in vivo mice model and has the ability of re-establishing the blood cells by boosting and stabilizing the blood parameters.

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“…Plant extracts are considered active when reduction or percentage suppression in parasitemia is ≥ 30% or signi cant prolonging the survival time of treated mice compared to the vehicle control (38)(39)(40). Thus, the leaf latex of A. weloensis was found to be active against P. berghei infected mice.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of in vitro and in vivo Antiplasmodial Activity of the Leaf Latex of Aloe Weloensis (Aloaceae)

Amare

Awgichew

Ahmed

et al. 2020

Preprint

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Background: Nature has gifted a variety of plants having potential effect against plasmodium parasites. The present study was aimed to determine in vitro and in vivo antimalarial activity of the leaf latex of Aloe weloensis.Methods: In vitro antimalarial activity of the leaf latex of A. weloensis was determined against 3D7 strain of P. falciparum. Antimalarial activity of the three doses the latex was evaluated in 4 day-suppressive and curative models against P. berghei infected mice. Antioxidant activity of the leaf latex of A. weloensis was assessed in 2,2- diphenyl 1- picrylhydrazine assay model. Results: Antioxidant activity of the latex was concentration dependent; the strongest inhibition was measured at 400 μg/mL (73.54%). The leaf latex of A. weloensis was demonstrated inhibitory activity against 3D7 malarial strain (IC50 = 9.14 μg/ml). Suppressive and curative effect of the latex was found to be dose dependent. Parasitemia reduction was significant (200 mg/kg, p<0.01, 400 and ,600 mg/kg, p<0.001) in 4-day suppressive test compared to vehicle control. Parasitemia level of the mice treated with 200, 400 and 600 mg/kg doses of the latex significantly (p<0.001) reduced with suppression of 36%, 58% and 64% respectively in curative test. Administration of the leaf latex of A. weloensis significantly (p<0.01) improved mean survival time, pack cell volume, rectal temperature and body weight of P. berghei infected mice. Conclusion: The finding showed that the leaf latex of Aloe weloensis endowed prominent antimalarial and antioxidant activities. The result can serve as a step towards the development of safe and effective herbal therapy against plasmodium parasites.

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Antiplasmodial activity of Indigofera spicata root extract against Plasmodium berghei infection in mice

Cited by 31 publications

References 28 publications

Antimalarial Activity of the Methanol Aerial Extract of Alysicarpus glumaceus (Vahl) DC. in Plasmodium berghei Infected Mice

Antimalarial Activity of the Methanol Aerial Extract of Alysicarpus glumaceus (Vahl) DC. in Plasmodium berghei Infected Mice

Malaria parasite clearance rate of crude methanol extract of Cryptolepis sanguinolenta in mice infected with chloroquine sensitive strain of Plasmodium berghei

Evaluation of in vitro and in vivo Antiplasmodial Activity of the Leaf Latex of Aloe Weloensis (Aloaceae)

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