Antiplasmodial and cytotoxic activities of Rwandan medicinal plants used in the treatment of malaria

Muganga, Raymond; Angenot, Luc; Tits, Monique; Frédérich, Michel

doi:10.1016/j.jep.2009.12.023

Cited by 77 publications

(58 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A significant activity was obtained with aqueous extract of Terminalia macroptera with IC 50 =1 μg/mL tested on P. falciparum CQ-resistant W2 strain (Sanon et al 2003). The methanolic and aqueous root bark extracts of Terminalia mollis were active against CQ-sensitive strain of P. falciparum (3D7) with IC 50 values of 11.7 and 33.5, respectively (Muganga et al 2010). The antiplasmodial activity was already known in Terminalia bellirica on the 3D7 strain (Valsaraj et al 1997).…”

Section: Resultsmentioning

confidence: 99%

Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Bagavan¹,

Rahuman²,

Kamaraj³

et al. 2010

Parasitol Res

View full text Add to dashboard Cite

The absence of a vaccine and the rampant resistance to almost all antimalarial drugs have accentuated the urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. The aim of the study was to discover effective plant extracts against Plasmodium falciparum. In the present study, the hexane, chloroform, ethyl acetate, acetone, and methanol extracts of Citrus sinensis (peel), Leucas aspera, Ocimum sanctum, Phyllanthus acidus (leaf), Terminalia chebula (seed) were tested for their antimalarial activity against chloroquine (CQ)-sensitive (3D7) strain of P. falciparum which was cultured following the candle-jar method. Antimalarial evaluations of daily replacement of culture medium containing CQ and different plant crude extracts were performed on 96-well plates at 37°C for 24 and 48 h. Parasitemia was determined microscopically on thin-film Giemsa-stained preparations. Plant extracts were tested for their cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay on human laryngeal cancer cell line (HEp-2) and normal cell line (Vero). Out of the 25 extracts tested, six showed good (IC(50) 4.76-22.76 μg/mL), 15 exhibited moderate (IC(50) 31.42-88.03 μg/mL), while four displayed mild (IC(50) > 100 μg/mL) antiplasmodial activity. The leaf ethyl acetate and methanol extracts of L. aspera; ethyl acetate, acetone, and methanol extracts of P. acidus; and seed acetone extract of T. chebula had good antiplasmodial activity (IC(50) = 7.81, 22.76, 9.37, 14.65, 12.68, and 4.76 μg/mL) with selectivity indices 5.43, 2.04, 4.88, 3.35, 3.42, and 9.97 for HEp-2 and >5.79, >2.20, >11.75, >3.41, >3.94, and >7.38 for Vero cells, respectively. These analyses have revealed for the first time that the components present in the solvent extracts of L. aspera, P. acidus, and T. chebula have antiplasmodial activity. The high antiplasmodial activity observed make these plants good candidates for isolation of anti-protozoal compounds which could serve as new lead structures for drug development.

show abstract

Section: Resultsmentioning

confidence: 99%

Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Bagavan¹,

Rahuman²,

Kamaraj³

et al. 2010

Parasitol Res

View full text Add to dashboard Cite

show abstract

“…Moreover, the difference could also be explained by the test procedure which was different [24]. Except these properties, this plant has been shown to have antioxidant properties [24], anticancer activities [24,27] antihelmentic activities [28], diuretic and analgesic effects [29], antiplasmodial properties [30].…”

Section: Cussonia Arborea (Ca) Dissotis Longipetala (Dl Lonchocarpusmentioning

confidence: 99%

Antimicrobial Activities of Some Medicinal Plants Against Multiresistants Microorganisms Causing Urogenital Tract Infections in Cameroon

Merveille¹

2017

JDMP

View full text Add to dashboard Cite

Abstract:Urogenital tract infections remain a serious global treat especially in women. The control of these infections is increasingly complicated due to development of resistance against available drugs. Therefore a perpetual search of new antimicrobial molecules is needed to face the challenge of microbial resistance. This study was initiated to screen the antimicrobial activities of methanol extracts of nine Cameroonian medicinal plants, used for the treatment of infectious diseases, against multiresistant pathogens isolated from urogenital infected patients. The plants included: Cussonia arborea, Dissotis longipetala, Lonchocarpus sepium, Nauclea pobeguinii, Picralima nitida, Rumex abyssinicus, Rumex berqueatii, Sapium ellypticum, Psorospermum febrifigum, They were tested on seven strains including six clinical isolates (Escherichia coli, Proteus vulgaris, Providencia stuartii, Pseudomonas aeruginosa, Staphylococcus saprophiticus, Candida krusei) and one reference strain (E. coli JM109). Clinical strains were checked for their multiresistance using disc diffusion method. The antimicrobial activities were determined by agar well diffusion method. MICs were determined using microdilution assay. The phytochemical screening of plants was also done. All the bacteria strains were found to be multidrug-resistant (MDR) against at least 7 of the 12 antibiotics tested. Antimicrobial activities demonstrated that 6/9 (SE, DL, RA, RB, PN, LS) plants were active on at least six microorganisms. MICs ranged from 0.125 to 128mg/ml for crude extracts, from 0.5 to 0.16µg/ml for gentamicin and was 0,002mg/ml for fluconazole. One extract (NP) which showed limited results on agar, inhibited the growth of all the strains with MICs ranging from 1to 16mg/ml showing a limited activity of this extract on agar. We found that four extracts (SE, NP, RA, DL) have significant activities since they presented MICs ≤ 8mg/ml on at least 5 tested microorganism individually. Findings from phytochemical screening showed that most active extracts contain tannins, alkaloids and saponins which could be responsible of these activities.

show abstract

“…Several chemical structures of plants from the DRC had high in vitro activities against various strains of P. falciparum (IC 50 < 1 µg/ml): strychnogucine B, ancistrocongoline A, isochondodendrine, ancistrocongoline B, cocsoline, korupensamine, ancistroealaine B, ellagic acid, physalin B, ancistrolikokine D, cryptolepine, hydroxycryptolepine, neocryptolepine and yadanziolide (Memvanga et al, 2015). Muganga et al (2010) explained that F. africana was known to possess ferruginol, a compound with strong antimalarial activity (IC 50 of 1.95 µg/ml). But ferruginol is not a desirable antimalarial candidate because it is highly cytotoxic.…”

Section: Plants Used In More Than Two Countriesmentioning

confidence: 99%

Plants as antimalarial agents in Sub-Saharan Africa

Chinsembu

2015

Acta Tropica

View full text Add to dashboard Cite

Antiplasmodial and cytotoxic activities of Rwandan medicinal plants used in the treatment of malaria

Cited by 77 publications

References 29 publications

Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Antimicrobial Activities of Some Medicinal Plants Against Multiresistants Microorganisms Causing Urogenital Tract Infections in Cameroon

Plants as antimalarial agents in Sub-Saharan Africa

Contact Info

Product

Resources

About