Antiplasmodial, anti-inflammatory and cytotoxic activities of various plant extracts from the Mascarene Archipelago

Jonville, Marie-Caroline; Kodja, Hippolyte; Strasberg, Dominique; Pichette, André; Ollivier, Évelyne; Frédérich, Michel; Angenot, Luc; Legault, Jean

doi:10.1016/j.jep.2010.06.013

Cited by 52 publications

(26 citation statements)

References 29 publications

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“…Ethyl acetate and dichloromethane crude extracts and hydroxy-γ-isosanshool (purity 90.58 %) were tested in vitro against the Plasmodium falciparum 3D7 strain. In line with WHO guidelines and previous results from our team (Jansen et al [10], Jonville et al [11]), antiplasmodial crude extract activity was classified as follows: IC 50 ≤ 15 µg/mL, promising activity; IC 50 = 15-50 µg/mL, moderate activity; IC 50 > 50 µg/mL, weak activity; and at a level that cannot explain the existence of antiplasmodial activity in the plant: IC 50 > 100 µg/mL, inactivity. The dichloromethane crude extract showed weak activity (77.8 ± 7.3 µg/mL), the ethyl acetate extract showed moderate activity (38.0 ± 11.3 µg/mL), and hydroxy-γ-isosanshool showed moderate activity for a pure compound (11.3 ± 1.5 µg/mL).…”

supporting

confidence: 90%

Phytochemical Profile and Biological Activity Evaluation of Zanthoxylum heterophyllum Leaves against Malaria

Ledoux¹,

Maraetefau²,

Jansen³

et al. 2015

Planta Med Lett

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!The aim of this study was to evaluate the antiplasmodial properties of Zanthoxylum heterophyllum, an endemic plant from the Mascarene Islands. In vitro antiplasmodial activity of ethyl acetate and dichloromethane crude extracts obtained from leaf samples collected on Reunion Island was evaluated on the Plasmodium falciparum 3D7 chloroquine-sensitive strain using a colorimetric method. The major active compound was identified by chromatographic and spectroscopic methods. The best antiplasmodial activity was obtained for the ethyl acetate extract (15 µg/ mL < IC 50 < 50 µg/mL). The major compound was identified as a sanshool derivative, an alkylamide compound that has moderate antimalarial activity (IC 50 = 11.3 µg/mL). This is the first report of the presence of a sanshool derivative in Z. heterophyllum. The moderate antiplasmodial activity of hydroxy-γ-isosanshool was demonstrated for the first time. Key wordsZanthoxylum heterophyllum · Rutaceae · malaria · antiplasmodial activitiy · Réunion Island · sanshool Supporting information available online at http://www.thieme-connect.de/products According to the last World Malaria Report [1], there were an estimated 627 000 malaria deaths worldwide in 2012. Malaria is caused by a parasite Plasmodium sp. and transmitted by Anopheles mosquitoes. The problem of parasite resistance towards available medicines such as chloroquine is increasing. Natural products could play an important role in the discovery of new antimalarial drugs. Indeed, the vegetal kingdom is an important source of new pharmacological active compounds, especially for the search of new antimalarial drugs as reviewed by Bero and al. [2]. Zanthoxylum heterophyllum Sm. is an endemic plant from Reunion Island, which belongs to the Rutaceae family [3]. In the traditional pharmacopoeia of the island, Z. heterophyllum, commonly named "poivrier des hauts", is used for the treatment of backaches [4]. As far as we know, pharmacological properties of this plant have not been studied before, except in a paper where methanol leaf and stem extracts showed free radical scavenging properties [5]. Nevertheless, some Zanthoxylum species are known to have antiplasmodial properties, such as Zanthoxylum chalybeum [6] and Zanthoxylum zanthoxyloides [7]. Results and Discussion !The major compound present in the ethyl acetate crude extract was identified as a sanshool derivative. Some sanshool derivates were already described in Zanthoxylum sp., such as Z.anthoxylum piperitum [8] and Z.anthoxylum integrifoliolum [9]. By comparison of our NMR and MS data with literature data, it was identified as hydroxy-γ-isosanshool (l " Fig. 1), described by Chen et al. [9]. Ethyl acetate and dichloromethane crude extracts and hydroxy-γ-isosanshool (purity 90.58 %) were tested in vitro against the Plasmodium falciparum 3D7 strain. In line with WHO guidelines and previous results from our team (Jansen et al. [10], Jonville et al. [11]), antiplasmodial crude extract activity was classified as follows: IC 50 ≤ 15 µg/mL, promising activ...

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supporting

confidence: 90%

Phytochemical Profile and Biological Activity Evaluation of Zanthoxylum heterophyllum Leaves against Malaria

Ledoux¹,

Maraetefau²,

Jansen³

et al. 2015

Planta Med Lett

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“… Whole plant β -caryophyllene, precocene I, friedelin, Lycopsamine, echinatine, β -sitosterol, stigmasterol, 5-methoxynobiletin, linderoflavone B, eupalestin, sabinene, α and β pinene, β -phellandrene, 1,8-cineole and limonene, ocimene, eugenol [ 35 ]. Antimicrobial, anticonvulsant, analgesic, anti-inflammatory, antipyretic, insecticidal, antioxidant, antiplasmodial, cytotoxic [ 35 , 37 , 38 ]. EUPHORBIACEAE /Croton macrostachys Hochst.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial activity of six medicinal Cameroonian plants against Gram-positive and Gram-negative multidrug resistant phenotypes

Voukeng

Beng

Kuete

2016

BMC Complement Altern Med

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BackgroundInfectious diseases due to multidrug-resistant bacteria are one of the causes of treatment failures contributing to an increase in mortality and/or morbidity. In this study, we evaluated the antibacterial potential of different parts of six medicinal plants namely Alstonia boonei, Ageratum conyzoides, Croton macrostachys, Cassia obtusifolia, Catharanthus roseus and Paullinia pinnata against a panel of 36 multi-drug resistant (MDR) Gram-negative and Gram-positive bacteria.MethodsMinimum Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of the methanol extracts from different parts of the plants were determined using broth microdilution method; standard phytochemical methods were used for phytochemical screening.ResultsSeveral phytochemical classes such as polyphenols, sterols, triterpenes, alkaloids, flavonoids and saponins were identified in the plant extracts. MIC values obtained ranged from 64 to 1024 μg/mL. Leaves extract of Catharanthus roseus (86.11 %), Croton macrostachys (83.33 %) and Paullinia pinnata (80.55 %) displayed the best antibacterial spectra. The lowest MIC value of 64 μg/mL was obtained with the Paullinia pinnata stems extract and Cassia obtusifolia extract against the strain of Staphylococcus aureus MRSA8. Results also showed that the tested samples generally displayed bacteriostatic effects with MBC values obtained in only 3.35 % of the cases where plant extracts were active.ConclusionThe results obtained at the end of this study demonstrate for the first time the antibacterial activity of the studied medicinal plants against MDR bacteria. The tested plants could be a reservoir of molecules to fight against MDR bacterial infections.

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“…The inhibition of NO production (%) in the treatment with 10 µM of compounds 1, 2, 5, 6, 7, and 8 was 51, 42, 28, 35, 80, and 57%, respectively. Jonville et al (2011) also found that dichloromethane extract from the leaf of H. benghalensis suppressed NO production (IC50 = 38 µg/mL) in LPS-induced RAW 264.7 macrophages. Reyes et al (2006) …”

Section: Effects Of Isolated Compounds On Lps-induced No Production Imentioning

confidence: 82%

“…The ethanolic extract of the stem of H. benghalensis showed high inhibitory effect on phosphodiesterase (Temkitthawon et al, 2008). Jonville et al (2011) indicated that dichloromethane extract from the leaf of H. benghalensis inhibited LPS-induced NO production (IC50 = 38 µg/mL) in RAW 264.7 macrophages. Temkitthawon et al (2008) indicated that ethanolic extract from the stem of H. benghalensis showed potent inhibitory activity on phosphodiesterase (IC50 = 2.75 µg/mL).…”

Section: Introductionmentioning

confidence: 97%

Anti-inflammatory effects of triterpenes and steroid compounds isolated from the stem bark of Hiptage benghalensis

Hsu

Fang

Huang

et al. 2015

Journal of Functional Foods

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Available online A B S T R A C TIn this study, we investigated the anti-inflammatory effects of triterpenes and steroid derivative isolated from the stem bark of Hiptage benghalensis in LPS-stimulated RAW 264.7 macrophages. In the extraction and isolation section, the chloroform-soluble fraction of the stem bark of H. benghalensis led to the isolation of seven triterpenes and a steroid derivative. These compounds were characterized as alnus-5(10)-en-3β-yl acetate (1), oleanan-3-one (2), 3β-acetoxy-9β-bauer-7-en-6-one (3), lupeol (4), (24R)-24-propylcholesterol (5), alnus-5(10)-en-3β-ol (6), 3β-acetoxy-20-hydroxylupane (7), and betulonic acid (8) by spectroscopic methods. In vitro anti-inflammatory activity, compounds 1, 2, 5, 6, 7, and 8 significantly inhibited the productions of NO and PGE2, and protein expressions of iNOS and COX-2 in LPS-stimulated RAW 264.7 macrophages. Compounds 1, 2, 7, and 8 also significantly increased the protein expression of IκBα, and decreased the protein expression of p-p65 and transcriptional activity of NF-κB in LPS-stimulated RAW 264.7 macrophages. Taken together, the present study clearly demonstrates that compounds isolated from the stem bark of H. benghalensis exhibit anti-inflammatory activity through the suppression of NF-κB in LPS-stimulated RAW 264.7 macrophages.

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Antiplasmodial, anti-inflammatory and cytotoxic activities of various plant extracts from the Mascarene Archipelago

Cited by 52 publications

References 29 publications

Phytochemical Profile and Biological Activity Evaluation of Zanthoxylum heterophyllum Leaves against Malaria

Phytochemical Profile and Biological Activity Evaluation of Zanthoxylum heterophyllum Leaves against Malaria

Antibacterial activity of six medicinal Cameroonian plants against Gram-positive and Gram-negative multidrug resistant phenotypes

Anti-inflammatory effects of triterpenes and steroid compounds isolated from the stem bark of Hiptage benghalensis

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