Antiplasmodial, antinociceptive and antipyretic potential of the stem bark extract of Burkea africana and identification of its antiplasmodial-active fraction

Abstract

Background and aim Burkea africana stem bark is used as a remedy for malaria in north-central and southern Nigeria. Based on its traditional use, this study was conducted to investigate the antiplasmodial, antinociceptive and antipyretic potential of an extract of B. africana stem bark. Experimental procedure A 70% v/v ethanol extract of stem bark of B. africana was prepared by cold maceration. Fractions (dichloromethane, ethyl acetate, and residual) were also prepared. The extract was screened for hemolytic, cytotoxic and antiplasmodial activity effects. The effect of the extract and fractions against chloroquine-sensitive (3D7) and multi-drug resistant (W2mef) P. falciparum was assessed. Acute toxicity test, acetic acid-induced abdominal writhing in mice, and lipopolysaccharide-induced fever in rats were also employed to screen the extract. Chromatographic fingerprints of the extract and active fraction were obtained. Results B. africana extract showed no cytotoxic or significant hemolytic effects and did not cause acute toxicity or mortality. The ethanol extract exhibited moderate antiplasmodial activity while the dichloromethane fraction showed high activity against P. falciparum 3D7 (IC 50 = 6.44 μg/ml) and W2mef (IC 50 = 6.30 μg/ml) respectively. The extract elicited significant ( p < 0.05) attenuation of acetic acid-induced writhing and significantly ( p < 0.05) ameliorated lipopolysaccharide-induced pyrexia at 300 mg/kg. The HPLC profile of the dichloromethane fraction showed peaks with retention times that corresponded with those of rutin and caffeic acid. Conclusion Burkea africana extract has antiplasmodial, antinociceptive and antipyretic potential and its antiplasmodial constituents are concentrated in its dichloromethane fraction.

The Potential of Plants of Family Fabaceaewith Emphasis on Putri Malu Medicinal Plant ‘Mimosa Pudica’ (Fabaceae) as an Antimalarial & an Insecticide for Malaria Vectors: A Review

“…Among all the tests, aqueous, methanolic, and water/ methanol extracts of aerial partderived showed the most promising results against P. falciparum FCR-3 strain, considering its IC50 value was less than 10 µg/mL. 30,59 The phytochemical screening of ethanolic extract revealed the presence of flavonoids and alkaloids. Phytochemical screening of other extracts or further purification of active compounds has not been carried out (Table 2).…”

Assessment of the In Vitro Cytotoxicity Effects of the Leaf Methanol Extract of Crinum zeylanicum on Mouse Induced Pluripotent Stem Cells and Their Cardiomyocytes Derivatives

“…This cell line expresses enhanced green fluorescent protein (EGFP) under the control of α-MHC promoter, which specifically allows the generation of highly pure CMs. To maintain their undifferentiated state as previously described [22], miPSCs were cultured on mitomycin Cinactivated MEFs in medium composed of Dulbecco's Modified Eagle's Medium (DMEM), supplemented with nonessential amino acids (0.1 mM), L-glutamine (2 mM), penicillin, streptomycin (50 µg/mL each), β-mercaptoethanol (0.1 mM), leukemia inhibitory factor (LIF) (500 U/mL) (ESGRO, Millipore; Schwalbach, Germany), and 15% fetal calf serum (FCS). Cells were passaged every 2-3 days and further used for differentiation or proliferation assays.…”