Antiplasmodial, Antioxidant, and Cytotoxic Activity of <i>Bridelia micrantha</i> a Cameroonian Medicinal Plant Used for the Treatment of Malaria

Kevin, Tako Djimefo Alex; Cédric, Yamssi; Nadia, Noumedem Anangmo Christelle; Sidiki, Ngouyamsa Nsapkain Aboubakar; Azizi, Mounvera Abdel; Guy-Armand, Gamago Nkadeu; Sandra, Tientcheu Noutong Jemimah; Nchetnkou, Christian Mbohou; Géraldine, Essangui Same Estelle; Tankoua-Tchounda, Roméo; Payne, Vincent Khan; Lehman, Léopold G.

doi:10.1155/2023/1219432

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…A substance is declared active if it reduces parasitaemia by at least 30% according to [ 18 ]. Previously, in vitro activity of B. micrantha plant bark extract against P. falciparum has been reported [ 8 ], suggesting that the plant may possess antimalarial compounds. The fact that the extracts increased the mean survival time suggests that they repressed P. berghei and lessened the parasite's overall pathologic impact on the mice.…”

Section: Discussionmentioning

confidence: 99%

“…The bark of the Cameroonian medicinal plant Bridelia micrantha is used to treat malaria by the population in the Noun Division of the Western Region of Cameroon. Antiplasmodial antioxidant activity and in vitro cytotoxicity on red blood cells (RBC) and macrophage cells have been demonstrated by [ 8 ]. Because of the possibility of the extract being physiologically active in vitro and inert in vivo as a result of biotransformation and bioavailability processes, it is crucial to conduct an in vivo investigation on B. micrantha .…”

Section: Introductionmentioning

confidence: 99%

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Antimalarial Efficacy of Ethanol Extract of Bridelia micrantha Stem Bark against Plasmodium berghei-Infected Mice

Kevin,

Cedric,

Nadia

et al. 2024

Journal of Parasitology Research

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Background. The spread of drug resistance is a significant issue, particularly in endemic countries with limited resources. The aim of this study was to evaluate antimalarial and antioxidant activity of B. micrantha in order to justify its use in traditional medicine. Methods. Evaluation of the in vivo antimalarial activity of B. micrantha was carried out according to the model of the suppressive and curative test of Peters’ over 4 days in infected Swiss albino mice. Antioxidant parameters and stress were measured after intraperitoneal administration of 1×107 infected red blood cells. Results. At doses of 150 mg/kg, 300 mg/kg, and 600 mg/kg, administration of B. micrantha substantially produced suppression of P. berghei infection by 67.75%, 73.46%, and 78.99%, respectively, while 84.64% of the untreated group (1% DMSO) had suppression from chloroquine. The curative test significantly decreased the levels of parasitaemia and death in the treated groups. Furthermore, after B. micrantha extract was given to infected mice, a noteworthy increase in total protein, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) was observed. On the other hand, hepatic catalase (CAT) and superoxide dismutase (SOD) productions were considerably greater than that of the healthy control. Mice had considerably lower levels of nonenzymatic antioxidant markers such as glutathione, NO, and MDA showing that the liver was protected. Conclusion. The infected groups responded favorably to the ethanol extract of B. micrantha. This result justifies investigation for its use in Cameroon.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antimalarial Efficacy of Ethanol Extract of Bridelia micrantha Stem Bark against Plasmodium berghei-Infected Mice

Kevin,

Cedric,

Nadia

et al. 2024

Journal of Parasitology Research

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“…The fluorescence was measured using a fluorescence microplate reader at an excitation and emission wavelength of 485 and 538 nm, respectively. The resistance index (RI) was calculated using the formula (Kevin et al, 2023):…”

Section: In Vitro Antiplasmodial Activitymentioning

confidence: 99%

In vitro and in vivo antimalarial activities of the ethanol extract of Erythrina sigmoidea stem bark used for the treatment of malaria in the Western Region of Cameroon

Jemimah Sandra,

Christelle Nadia,

Cedric

et al. 2024

Front. Parasitol.

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BackgroundMalaria is one of the leading causes of morbidity and/or mortality in tropical Africa. The spread and development of resistance to chemical antimalarial drugs and the relatively high cost of the latter are problems associated with malaria control and are reasons to promote the use of plants to meet healthcare needs to treat malaria. The aim of this study was to evaluate antiplasmodial activities of extracts of Erythrina sigmoidea (Mah quat), which is traditionally used for the treatment of malaria in the western region of Cameroon.Material and methodsThe ethanol extract of E. sigmoidea stem bark was obtained through the maceration process using 95% ethanol, while the aqueous extract was prepared by infusion. The in vitro antiplasmodial effect of extracts against P. falciparum chloroquine-sensitive (3D7) and chloroquine-resistant (Dd2) strains was determined using the Trager and Jensen method. On the other hand, the in vivo antimalarial activity of the extract was evaluated in mice infected with Plasmodium berghei strain NK65 using the Peters’ 4-day suppressive test and Ryley test (curative test). A total of 36 mice were used, subdivided into six groups of six mice each: one normal control, a negative control, a positive control, and three other groups for the tested product. Blood samples were collected on the 10th day of each test for hematological parameters.ResultsThe aqueous extract had an in vitro antiplasmodial activity against the chloroquine-sensitive strain with an IC50 of 29.51 ± 3.63 µg/mL and against the chloroquine-resistant strain with an IC50 of 35.23 ± 3.17 µg/mL. The highest in vitro antiplasmodial activity was observed with the ethanol extract against the chloroquine-sensitive strain with an IC50 of 6.44 ± 0.08 µg/mL and against the chloroquine-resistant strain with an IC50 of 7.53 ± 0.22 µg/mL. The ethanol extract demonstrated suppressive activity in vivo with reduction rates of 87.69%, 86.79%, and 81.08% at doses of 500 mg/kg, 250 mg/kg, and 125 mg/kg, respectively; and curative activity in vivo with reduction rates of 80%, 78.5%, and 77.5% at doses of 500 mg/kg, 250 mg/kg, and 125 mg/kg, respectively. The number of white blood cells in the negative control (44.55 ± 5.02 103/µL) was higher compared to the other groups. As for the red blood cells, we observed a massive destruction of the latter in the infected and untreated group (5.82 ± 1.50 106/µL) compared to the infected and ethanol extract-treated groups (8.74 ± 1.57 106/µL for 500 mg/kg, 7.54 ± 1.77 106/µL for 250 mg/kg, and 8.9 ± 1.50 106/µL for 125 mg/kg).ConclusionThis study provides scientific data on the use of E. sigmoidea by the local population for the treatment of malaria. It shows that E. sigmoidea has antiplasmodial activity, and we also see that there are differences between the parameters that we have in the treated groups and those of the untreated group. However, toxicity tests are necessary to assess its safety.

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In vitro and in vivo evaluation of the antimalarial, anti-inflammatory and free radical scavenging potentials of the hydro-alcoholic leaf extract of Microdesmis puberula Hook. F. ex Planch (Pandaceae)

Adjei-Hinneh,

Zoiku,

Asante-Kwatia

et al. 2024

Vegetos

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Antiplasmodial, Antioxidant, and Cytotoxic Activity of Bridelia micrantha a Cameroonian Medicinal Plant Used for the Treatment of Malaria

Cited by 6 publications

References 29 publications

Antimalarial Efficacy of Ethanol Extract of Bridelia micrantha Stem Bark against Plasmodium berghei-Infected Mice

Antimalarial Efficacy of Ethanol Extract of Bridelia micrantha Stem Bark against Plasmodium berghei-Infected Mice

In vitro and in vivo antimalarial activities of the ethanol extract of Erythrina sigmoidea stem bark used for the treatment of malaria in the Western Region of Cameroon

In vitro and in vivo evaluation of the antimalarial, anti-inflammatory and free radical scavenging potentials of the hydro-alcoholic leaf extract of Microdesmis puberula Hook. F. ex Planch (Pandaceae)

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