Antiplasmodial Assessment of Artemether- Lumefantrine/Ivermectin on Mice Infected with Plasmodium berghei

Georgewill, Udeme Owunari; Liverpool, Ebiladei; Adikwu, Elias

doi:10.5530/ajbls.2020.9.55

Cited by 2 publications

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“…The current study observed altered lipid levels in P. berghei infected mice marked by increased TG, TCHOL, and LDL-C and decreased HDL-C levels. Similarly, Georgewill et al [28] reported altered lipid profile in P. berghei infected mice. In this study, treatment with S/P/D restored lipids levels best than individual doses of D, S/P and CQ.…”

Section: Discussionmentioning

confidence: 83%

In-vivo Antiplasmodial Activity of Sulfadoxine/Pyrimethamine/Doxycycline on Plasmodium berghei Infected Mice

Georgewill

Adikwu

2021

JABB

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The search for newer antimalarial drug combinations is on the front burner due to rising Plasmodium resistance to some currently used antimalarial drugs. This study examined the antiplasmodial activity of sulfadoxine/pyrimethamine/doxycycline (S/P/D) on mice infected with Plasmodium berghei (P. berghei). Swiss albino mice (25-30 g) inoculated with P. bergei (1x107) were treated with D (2.2 mg/kg), S/P (21.4/10.7 mg/kg), and S/P/D for 4 days. The positive and negative controls were treated with normal saline (0.2 ml) and chloroquine (CQ) (10 mg/kg) for 4 days, respectively. After treatment, blood samples were collected and assessed for parasitemia levels and biochemical parameters. The mice were observed for mean survival time (MST). D, S/P, S/P/D and CQ significantly decreased parasitemia in the curative, prophylactic and suppressive tests at p<0.05; p<0.01, p<0.001 and p<0.001, respectively when compared to negative control. In the curative study, 55.9%, 65.1%, and 81.7% parasitemia inhibitions were produced by D, S/P and S/P/D, respectively whereas CQ produced 75.6 % parasitemia inhibition. D, S/P and S/P/D significantly prolonged MST at p<0.05, p<0.01 and p<0.001 respectively when compared to negative control. Altered serum biochemical markers in P. berghei infected mice were marked by significantly (p<0.001) decreased packed cell volume, red blood cells, hemoglobin, high density lipoprotein cholesterol levels with significantly (p<0.001) increased cholesterol, white blood cells, total cholesterol, low-density lipoprotein cholesterol and triglyceride levels when compared to control. However, D, S/P and S/P/D significantly restored the aforementioned markers at p<0.05, p<0.01 and p<0.001, respectively when compared to negative control. S/P/D may be used as an antimalarial drug.

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Section: Discussionmentioning

confidence: 83%

In-vivo Antiplasmodial Activity of Sulfadoxine/Pyrimethamine/Doxycycline on Plasmodium berghei Infected Mice

Georgewill

Adikwu

2021

JABB

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“…The mice were kept in cages under normal environmental conditions and were acclimated for 2 weeks with free access to feeds and water. The following doses of drugs were used: CQ (10 mg/kg) [18], NT (57.1 mg/kg) [19] and DP (1.71/13.7mg/kg) [20].…”

Section: Drugs and Experimental Animalsmentioning

confidence: 99%

In-vivo Antiplasmodial Effect of Dihydroartemisinin-Piperaquine-Nitrofurantoin on Plasmodium berghei- Infected Mice

Georgewill

Joseph

Adikwu

2021

JAMPS

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Nitrofurantoin (NT) used for the treatment of urinary tract infections may have antiplasmodial activity. Dihydroartemisinin-piperaquine (DP) is an artemisinin based combination therapy used for the treatment of malaria. This study evaluated the antiplasmodial effect of dihydroartemisinin-piperaquine-nitrofurantoin (DP-NT) on mice infected with Plasmodium berghei. Adult Swiss albino mice (30-35 g) of both sexes were used. The mice were randomly grouped, inoculated with Plasmodium berghei, and treated orally with DP (1.7/13.7 mg/kg), NT (57.1 mg/kg) and DP-NT (1.71/13.7/ 57.1 mg/kg), respectively using curative, prophylactic and suppressive tests. The negative control was orally treated with normal saline (0.3 mL), while the positive control was orally treated with chloroquine CQ (10mg/kg). After treatment, blood samples were collected and evaluated for percentage parasitemia, inhibitions and hematological parameters. Liver samples were evaluated for histological changes. The mice were observed for mean survival time (MST). Treatment with DP-NT decreased parasitemia levels when compared to individual doses of DP and NT with significant difference observed at p<0.05. DP-NT prolonged MST when compared to individual doses of DP and NT with significant difference observed at p<0.05. The decrease in packed cell volume, red blood cells, hemoglobin and increase in white blood cells in parasitized mice were significantly restored by DP-NT when compared to individual doses of DP and NT with difference observed at p<0.05. DP-NT eradicated liver Plasmodium parasite. NT remarkably increased the antiplasmodial activity of DP. DP-NT may be used for the treatment of malaria.

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Antiplasmodial Assessment of Artemether- Lumefantrine/Ivermectin on Mice Infected with Plasmodium berghei

Cited by 2 publications

References 0 publications

In-vivo Antiplasmodial Activity of Sulfadoxine/Pyrimethamine/Doxycycline on Plasmodium berghei Infected Mice

In-vivo Antiplasmodial Activity of Sulfadoxine/Pyrimethamine/Doxycycline on Plasmodium berghei Infected Mice

In-vivo Antiplasmodial Effect of Dihydroartemisinin-Piperaquine-Nitrofurantoin on Plasmodium berghei- Infected Mice

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