Antiproliferation of MP12 derived from a fungus, Aphanomyces invadans virulence factor, cysteine-rich trypsin inhibitor on human laryngeal epithelial cells, and in vivo zebrafish embryo model

Velayutham, Manikandan; Sarkar, Purabi; Rajakrishnan, R.; Kuppusamy, Palaniselvam; Juliet, Annie; Arockiaraj, Jesu

doi:10.1016/j.toxicon.2022.02.019

Cited by 12 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar observations have been reported previously in (i) A. invadans virulence protein, trypsin inhibitor-derived peptide inhibited human laryngeal cancer and (ii) Brevibacillus laterosporus isolated peptide, brevilaterin B that increases the LDH level, has been utilized to address epidermal cancer . Based on this supporting evidence and the outcomes of this study, we suggest that PS9 inhibits MCF-7 cell proliferation at a concentration range between 10 and 40 μM.…”

Section: Resultssupporting

confidence: 91%

“…A similar observation has been reported in the peptide, MP12, derived from A. invadans virulent molecule, a cysteine-rich trypsin inhibitor treated on human laryngeal epithelial cells . Hence, in the in vitro and in vivo models, PS9 is nontoxic.…”

Section: Resultsmentioning

confidence: 99%

“…Aspergillus fumigatus (invasive aspergillosis causing pathogenic fungus)-derived small molecule elicited anticancer activity . MP12, derived from the virulent fungal molecule trypsin inhibitor, showed significant anticancer properties against human laryngeal cancer cells (Hep-2) . The in vivo toxicity of the virulent peptide was screened in the zebrafish embryo model.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

PS9, Derived from an Aquatic Fungus Virulent Protein, Glycosyl Hydrolase, Arrests MCF-7 Proliferation by Regulating Intracellular Reactive Oxygen Species and Apoptotic Pathways

et al. 2023

Self Cite

View full text Add to dashboard Cite

One of the most common diseases in women is breast cancer, which has the highest death globally. Surgery, chemotherapy, hormone treatments, and radiation are the current treatment options for breast cancer. However, these options have several adverse side effects. Recently, peptide-based drugs have gained attention as anticancer therapy. Studies report that peptides from biological toxins such as venom and virulent pathogenic molecules have potential therapeutic effects against multiple diseases, including cancers. This study reports on the in vitro anticancer effect of a short peptide, PS9, derived from a virulent protein, glycosyl hydrolase, of an aquatic fungus, Aphanomyces invadans. This peptide arrests MCF-7 proliferation by regulating intercellular reactive oxygen species (ROS) and apoptotic pathways. Based on the potential for the anticancer effect of PS9, from the in silico analysis, in vitro analyses using MCF-7 cells were executed. PS9 showed a dose-dependent activity; its IC 50 value was 25.27−43.28 μM at 24 h. The acridine orange/ethidium bromide (AO/EtBr) staining, to establish the status of apoptosis in MCF-7 cells, showed morphologies for early and late apoptosis and necrotic cell death. The 2,7-dichlorodihydrofluorescein diacetate (DCFDA) staining and biochemical analyses showed a significant increase in reactive oxygen species (ROS). Besides, PS9 has been shown to regulate the caspase-mediated apoptotic pathway. PS9 is nontoxic, in vitro, and in vivo zebrafish larvae. Together, PS9 may have an anticancer effect in vitro.

show abstract

Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

PS9, Derived from an Aquatic Fungus Virulent Protein, Glycosyl Hydrolase, Arrests MCF-7 Proliferation by Regulating Intracellular Reactive Oxygen Species and Apoptotic Pathways

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Chiangjong et al [ 10 ] reported that the amino acid residues including Glu, Cys, Gly and Lys have anti-cancer properties; PS14 does have such amino acids in its sequence. An earlier study also found similar results, wherein MP12 peptide, from fungus virulent molecule cysteine-rich trypsin inhibitor showed significant anti-cancer properties against Hep-2 cells [ 8 ].…”

Section: Resultssupporting

confidence: 59%

“…Numerous reports demonstrate that the peptides derived from natural and animal sources show several biofunctional activity, such as immunomodulatory, antibacterial, antihypertensive, antithrombotic, anti-cancer, antioxidative and cholesterol-lowering properties [ 7 ]. Anti-cancer peptides (ACP) are molecularly targeted peptides that can penetrate and directly connect to the membranes of specific cancerous cells, organelles or binding peptides that deliver the anti-cancer medicines [ 8 ]. Depending on the number of amino acid residues, sequence length, composition, molecular weight, isoelectric point, net charge, hydrophobicity, amphiphilicity, secondary structure and structural orientation, peptides can be used as molecularly targeted drugs and ‘guiding missiles’ to inhibit cell proliferation or completely eliminate cancer cells [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Cancer and Anti-Inflammatory Activities of a Short Molecule, PS14 Derived from the Virulent Cellulose Binding Domain of Aphanomyces invadans, on Human Laryngeal Epithelial Cells and an In Vivo Zebrafish Embryo Model

et al. 2022

Self Cite

View full text Add to dashboard Cite

In this study, the anti-cancer and anti-inflammatory activities of PS14, a short peptide derived from the cellulase binding domain of pathogenic fungus, Aphanomyces invadans, have been evaluated, in vitro and in vivo. Bioinformatics analysis of PS14 revealed the physicochemical properties and the web-based predictions, which indicate that PS14 is non-toxic, and it has the potential to elicit anti-cancer and anti-inflammatory activities. These in silico results were experimentally validated through in vitro (L6 or Hep-2 cells) and in vivo (zebrafish embryo or larvae) models. Experimental results showed that PS14 is non-toxic in L6 cells and the zebrafish embryo, and it elicits an antitumor effect Hep-2 cells and zebrafish embryos. Anticancer activity assays, in terms of MTT, trypan blue and LDH assays, showed a dose-dependent inhibitory effect on cell proliferation. Moreover, in the epithelial cancer cells and zebrafish embryos, the peptide challenge (i) caused significant changes in the cytomorphology and induced apoptosis; (ii) triggered ROS generation; and (iii) showed a significant up-regulation of anti-cancer genes including BAX, Caspase 3, Caspase 9 and down-regulation of Bcl-2, in vitro. The anti-inflammatory activity of PS14 was observed in the cell-free in vitro assays for the inhibition of proteinase and lipoxygenase, and heat-induced hemolysis and hypotonicity-induced hemolysis. Together, this study has identified that PS14 has anti-cancer and anti-inflammatory activities, while being non-toxic, in vitro and in vivo. Future experiments can focus on the clinical or pharmacodynamics aspects of PS14.

show abstract

Molecular Docking of SA11, RF13 and DI14 Peptides from Vacuolar Protein Sorting Associated Protein 26B Against Cancer Proteins and In vitro Investigation of its Anticancer Potency in Hep-2 Cells

Velayutham

Guru

Gatasheh

et al. 2022

Int J Pept Res Ther

View full text Add to dashboard Cite

Antiproliferation of MP12 derived from a fungus, Aphanomyces invadans virulence factor, cysteine-rich trypsin inhibitor on human laryngeal epithelial cells, and in vivo zebrafish embryo model

Cited by 12 publications

References 34 publications

PS9, Derived from an Aquatic Fungus Virulent Protein, Glycosyl Hydrolase, Arrests MCF-7 Proliferation by Regulating Intracellular Reactive Oxygen Species and Apoptotic Pathways

PS9, Derived from an Aquatic Fungus Virulent Protein, Glycosyl Hydrolase, Arrests MCF-7 Proliferation by Regulating Intracellular Reactive Oxygen Species and Apoptotic Pathways

Anti-Cancer and Anti-Inflammatory Activities of a Short Molecule, PS14 Derived from the Virulent Cellulose Binding Domain of Aphanomyces invadans, on Human Laryngeal Epithelial Cells and an In Vivo Zebrafish Embryo Model

Molecular Docking of SA11, RF13 and DI14 Peptides from Vacuolar Protein Sorting Associated Protein 26B Against Cancer Proteins and In vitro Investigation of its Anticancer Potency in Hep-2 Cells

Contact Info

Product

Resources

About