Antiproliferative Activity and Characterization of Metabolites of Aspergillus nidulans: An Endophytic Fungus from Nyctanthes arbor-tristis Linn. Against Three Human Cancer Cell Lines

Abstract

In this paper the cytotoxicity of mycelium and broth constituents of endophytic fungus Aspergillus nidulans from Nyctanthes arbor-tristis is reported for the first time. The study shows that fungus Aspergillus nidulans from Nyctanthes arbor-tristis is capable of producing biologically active natural compounds and provides a scientific rationale for further chemical investigations into endophyte-producing natural products.

Natural product diversity from the endophytic fungi of the genusAspergillus

“…11. Aspergillus nidulansRecently in 2019, Sana et al isolated the endophytic fungus Aspergillus nidulans associated with Nyctanthes arbor-tristis Linn 25. Chemical investigation of the fermented culture of Aspergillus nidulans resulted in the isolation of the xanthone sterigmatocystin (66) (Fig.8), which was tested against the human cancer cell lines lung (NCI-H460), breast (MCF-7) and uterine cervix (HeLa).…”

“…Chemical investigation of the fermented culture of Aspergillus nidulans resulted in the isolation of the xanthone sterigmatocystin (66) (Fig.8), which was tested against the human cancer cell lines lung (NCI-H460), breast (MCF-7) and uterine cervix (HeLa). Sterigmatocystin (66) demonstrated cytotoxic activity against breast (MCF-7) cell line only with IC 50 value of 50 AE 2.5 mM mL À1 25.…”

Bioactive Natural Products from Endophytic Fungus Aspergillus nidulans Associated with Nyctanthes arbor-tristis Linn.

“…2) and several small fractions. Fr.1 eluted with petroleum ether-acetone (9.9-0.1) and afforded a pure compound identified as 5, 10-dihydrophenazine-1-carboxylic acid (2; 9.0 mg) reported earlier by our group [11]. Fr.…”

Endophytic Fungi: An Effective Alternative Source of Plant-Derived Bioactive Compounds for Pharmacological Studies

“…This discovery aroused the attention of mycologists and pharmaceutical chemists on endophytic fungi as a new source of bioactive substances and stimulated the interest in endophytic fungi as a sustainable source of plant metabolites. As shown in Table 1, many compounds that were isolated from endophytic fungi were also identified in some plant species as well as exhibited similar biological activity even though there were isolated from different sources, confirming endophytic fungi as an alternative source of bioactive compounds [19][20][21][22][23][24][25][26][27][28][29][30][31][32]. An overview of the recent literature surveys revealed that 51% of the bioactive substances isolated from endophytic fungi were previously unknown, with about 38% being isolated from soil microbiota [19].…”