Antiproliferative activity of ferulic acid-encapsulated electrospun PLGA/PEO nanofibers against MCF-7 human breast carcinoma cells

Abstract

Ferulic acid (FA) is a polyphenolic phytonutrient which possesses strong antiproliferative effect; however, it has limited therapeutic applications due to its physiochemical instability and low bioavailability at the tumor site. In present study, these shortcomings associated with FA were overcome by fabricating FA-encapsulated poly(d,l-lactide-co-glycolide)/polyethylene oxide (PLGA/PEO) blend nanofibers using electrospinning technique. FESEM and fluorescence microscopic analysis imitates the smooth morphology and even distribution of FA within the polymeric nanofibers at optimum 2 wt% concentration of FA. The average diameters were recorded to be 150 ± 47.4 and 200 ± 79 nm for PLGA/PEO and FA-encapsulated PLGA/PEO nanofibers, respectively. The encapsulation, compatibility, and physical state of FA within the nanofibers were further confirmed by FTIR, TGA and XRD analysis. In vitro drug delivery studies demonstrated initial burst liberation of FA within 24 h followed by a sustained release for the subsequent time. MTT assay revealed the effectiveness of FA-encapsulated nanofibers against human breast carcinoma cells (MCF-7) cells as compared to control. FESEM and fluorescence microscopic analysis further confirmed the apoptotic effect of FA-encapsulated PLGA/PEO nanofibers against MCF-7. These fabricated nanofibers hold enormous potential to be used as a therapeutic agent for various biomedical applications.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-014-0229-6) contains supplementary material, which is available to authorized users.

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

“…Studies have revealed that phenolic compounds such as FA at higher concentrations may act as pro-oxidant interacting with transition metal ions found in biological systems which lead to oxidative damage of normal cellular components (Maurya and Devasagayam 2010;Galati and O'Brien 2004). Our research group too has reported that the viability of non-cancerous HEK-293 cell lines was lower in presence of native FA in comparison with the nanofiber encapsulated FA (Vashisth et al 2015). Data obtained in our investigation imply superior cytocompatibilty of FA/ CS-TPP NPs as compared to native FA at the same concentration (40 lM).…”

“…Due to increased bioavailability, FA exhibits its antitumor effect by influencing the mitochondrial activity and antioxidant status of the cell disrupting the cell organelle which would eventually lead to cell death. In our earlier reports, FA from Parthenium hysterophorus and FA encapsulated nanofibers were found to exhibit anticancer potential against different cell lines , Vashisth et al 2015. Zhou et al (2007) reported the 27 % inhibition of HeLa cervical cancer cell lines when treated with 500 mg/L chitosan nanoparticles.…”

“…Each test formulation (40 lM native FA, unloaded CS-TPP NPs and 40 lM FA/CS-TPP NPs) was tested for its cytocompatibilty by means of HEK-293 cells using a previously described protocol (Vashisth et al 2015). Untreated cells were used as control; MTT assay and FESEM analysis (as described for ME-180 cells) were carried out following 24 h of cell seeding.…”

A novel gellan–PVA nanofibrous scaffold for skin tissue regeneration: Fabrication and characterization

“…XRD spectra of (a) raw gellan (b) raw PVA (c) gellan-PVA physical mixture (FESEM morphology of gellan-based (a) heat crosslinked gellan-PVA nanofibers (b) 15 nanofibers immersed in water for 1 day and (c) nanofibers immersed in water for 7 days 16. …”

“…M a n u s c r i p t 16 In this study, we fabricated novel gellan-based nanofibrous scaffolds using electrospinning 1 technique. These fabricated nanofibers proved to exhibit good uniformity, structural integrity and 2 cytocompatibility.…”