Antiproliferative Activity of Kenyan Trametes versicolor Aqueous Extract on Selected Cancer and Normal Cell Lines

JK, Chengo; Adipo, N; DM, Kiboi

doi:10.4172/1948-5956.1000427

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…The discrepancies in cytotoxic activity among mushroom extracts may be attributed in part to changes in compound polarity and solubility, bioactive component modes of action, and the presence of various cytotoxic chemicals in each extract [ 57 ]. In contrast to our findings, a prior investigation found that water extracts had extremely low cytotoxic activity on Vero cell proliferation [ 58 ]. This variation could be attributable to the geographical location and extraction procedures used in the current and earlier investigations.…”

Section: Discussioncontrasting

confidence: 99%

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Wild Mushrooms: Potential Natural Sources of Antioxidant and Anti‐Quorum Sensing Bioactive Compounds for Medical Applications

Gebreyohannes,

Sbhatu,

Nyerere

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Objective. This study was aimed at determining the antioxidant, anti-quorum sensing, and in vitro cytotoxic activities of five wild mushroom extracts. Methods. Wild mushrooms of Auricularia auricula-judae, Termitomyces umkowaani, Trametes elegans, Trametes versicolor, and Microporus xanthopus were collected from Arabuko-Sokoke and Kakamega National Forests, in Kenya. Specimens were identified and extracted using chloroform (CHL), 70% ethanol (Eth), and hot water (HW) solvents. Antioxidant and cytotoxic activities of the extracts were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Vero cell lines, respectively, while anti-quorum sensing activities were tested against Chromobacterium violaceum. All data were compared using relevant descriptive and inferential statistics at a significance level of p ≤ 0.05. Results. A total of 35 wild mushrooms were collected, identified, and classified into 14 genera. Among screened mycochemicals, fatty acids, flavonoids, polyphenols, and saponins were detected at higher concentrations. The highest free radical scavenging activities of A. auricula-judae, T. umkowaani, T. elegans, and T. versicolor were observed in 70% Eth extract with the percentage values of 76.40 ± 0.12%, 68.40 ± 0.01%, 62.40 ± 0.07%, and 66.40 ± 0.04%, respectively, whereas the HW extract of Microporus xanthopus showed free radical scavenging activity at 65.90 ± 0.02%. None of the extracts, at the tested concentrations (up to 1000 µg/mL), had shown cytotoxic activity against the Vero cell line. The HW extract of T. umkowaani and the 70% Eth extract of T. versicolor showed a statistically significant difference in the inhibitory activity of violacein production against C. violaceum at the concentration of 200 µg/mL. Conclusions. The antioxidant activity of wild mushrooms can help to tackle the diseases caused by free radicals. The anti-quorum sensing potential of wild mushrooms could also provide future alternatives to conventional drug therapies cost-effectively. Further detailed chemistry of the bioactive compounds and their possible mechanisms of action responsible for the observed antioxidant and anti-quorum sensing activities are needed.

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Section: Discussioncontrasting

confidence: 99%

“…In contrast to our fndings, a prior investigation found that water extracts had extremely low cytotoxic activity on Vero cell proliferation [58]. Tis variation could be attributable to the geographical location and extraction procedures used in the current and earlier investigations.…”

Section: Discussioncontrasting

confidence: 98%

Wild Mushrooms: Potential Natural Sources of Antioxidant and Anti‐Quorum Sensing Bioactive Compounds for Medical Applications

Gebreyohannes,

Sbhatu,

Nyerere

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Evaluation of Compounds Extracted from Eight Genera of Wild Mushrooms from Nigeria for Anti-cell Proliferation Activity in Vitro

Adongbede

Aduralere

2019

eijs

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Mushrooms have bioactive compounds that have antimicrobial, anti-cancer and antioxidant activities among other medicinal benefits. In the present study, we examined the anti-cell proliferation activities of mushrooms from eight genera obtained from the wild in Nigeria. Saccharomyces cerevisiae was used as a model organism to screen mushroom extracts for anti-cell proliferation activity. Polyphenols, high molecular weight polysaccharides and low molecular weight compounds from aqueous extracts were obtained from the test mushrooms using methanol and water respectively. The extracts were screened in vitro at different concentrations of extracts with the CyQuant cell proliferation assay. The high molecular weight polysaccharides from tested mushrooms reduced cell proliferation (96.79% inhibition in Ganoderma multipileum Ding Hou to 66.71% inhibition in Coltricia perennis (L.) Murrill at 10.00mg/ml). Percentage inhibition caused by low molecular weight compounds varied from 94.22% (Ganoderma multipileum) to 76.19% (Coltricia perennis) at 10mg/ml. Percentage of inhibition with the polyphenols varied from 94.12% (Microporus xanthopus Fr) Kuntze) to 79.82% (Coltricia perennis) at high doses. High molecular polysaccharides, low molecular weight compounds and polyphenols from mushrooms have anti-cancer properties. The CyQUANT assay proliferation kit was a very efficient tool for screening extracts from wild mushrooms for anti-cell proliferation activities. Medicinal mushrooms in Nigeria show a lot of promise as a reservoir for drug discovery particularly in the area of cancer research.

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Antiproliferative Activity of Kenyan Trametes versicolor Aqueous Extract on Selected Cancer and Normal Cell Lines

Cited by 2 publications

References 28 publications

Wild Mushrooms: Potential Natural Sources of Antioxidant and Anti‐Quorum Sensing Bioactive Compounds for Medical Applications

Wild Mushrooms: Potential Natural Sources of Antioxidant and Anti‐Quorum Sensing Bioactive Compounds for Medical Applications

Evaluation of Compounds Extracted from Eight Genera of Wild Mushrooms from Nigeria for Anti-cell Proliferation Activity in Vitro

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