Antiproliferative and apoptotic effects of silibinin in rat prostate cancer cells

Tyagi, Alpana; Bhatia, Neehar; Condon, Mark S.; Bosland, Maarten C.; Agarwal, Chapla; Agarwal, Rajesh

doi:10.1002/pros.10146

Cited by 81 publications

(54 citation statements)

References 22 publications

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“…The viability of the cells was determined using the trypan blue dye exclusion assay, in which the color changes reflected the dead cells (13). In brief, the cells (1x10 4 /ml) were first seeded in the cell culture flask (each concentration in triplicate).…”

Section: Plant Materials and Extraction And Isolation Of Ttb2mentioning

confidence: 99%

Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells

Huang

Zou

2015

Experimental and Therapeutic Medicine

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Abstract. The steroidal saponin TTB2 can be isolated from the n-BuOH extracts of Trillium tschonoskii Maxim. The aim of the present study was to observe whether this saponin exerted any cytotoxic effects on malignant sarcoma cells, and to further investigate the possible underlying molecular mechanisms. The cell viability, cell cycle arrest and phosphorylation of certain important signal molecules in the sarcoma cell line were investigated. It was found that TTB2 inhibited the growth of the Ewing sarcoma cell line and arrested cells in the G 2 /M and S phases of the cell cycle in a dose-and time-dependent manner. Furthermore, the phosphorylation of extracellular signal-regulated kinase was inhibited by TTB2. In conclusion, the results showed that the saponin TTB2 isolated from T. tschonoskii Maxim exerts anticancer effects and may be a potential candidate for the development of anticancer drugs for use in the treatment of cancer.

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Section: Plant Materials and Extraction And Isolation Of Ttb2mentioning

confidence: 99%

Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells

Huang

Zou

2015

Experimental and Therapeutic Medicine

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“…Recently, several studies with the antioxidant vitamin E and the polyphenolic flavonoid silibinin showed similar results concerning their anti-proliferation effects on PCA cells. The signaling pathway includes elevation in the hypophosphorylated form of pRb at the expense of the phosphorylated form of Rb, reduction at the protein level of CDKs, and cyclin typical to G1 phase of the cell cycle together with enhanced expression of the CDK inhibitor p21 cip1 [55][56][57].…”

Section: Discussionmentioning

confidence: 99%

Unique natural antioxidants (NAOs) and derived purified components inhibit cell cycle progression by downregulation of ppRb and E2F in human PC3 prostate cancer cells

et al. 2004

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Prostate cancer (PCA) is the leading cause of cancer mortality among older men in Western countries. Epidemiological studies have shown correlation between a lower risk of PCA and a higher consumption of antioxidants. However, the mechanism by which antioxidants exert their effects is still unknown. In the present study, we explored the signaling mechanism through which unique natural antioxidant derived from spinach extract (NAO) exerts their beneficial effects in the chemoprevention of PCA using human PC3 cells. Probing into the effect of NAO and its derived polyphenols on cell-cycle G1 arrest, we found that they cause cell-cycle prolongation. NAO and its two derived purified components exhibited a significant increase in the level of p21 cip1 expression after 36 h of starvation, followed by 18 h of treatment with NAO in the presence of serum. In addition, under similar conditions, the expressed level of Cyclin A and CDK-2 in the PC3 cells was significantly reduced after treatment with NAO or its purified components. Immunoblot analysis demonstrated a significant increase in the hypophosphorylated form of pRb and a decrease in ppRb. NAO and its purified derived components were found to downregulate the protein expression of another member of the pRb family, p107, as well as that of E2F-1. These results suggest that NAOinduced G1 delay and cell cycle prolongation are caused by downregulation of the protein expression of ppRb and E2F in the human PCA cell line PC3.

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“…The phytochemicals in parsley can slow the speed of cell division, leaving time for the cell to correct DNA mistakes and activate apoptosis and inhibit the migration of cancer cells. Considerable research shows that the flavonoid apigenin, found in celery, artichokes, and parsley may well be a key agent for killing breast, pancreatic, and numerous other cancer cell lines [23]. For example, 2015 research found apigenin to inhibit the growth of breast cancer cells, including HER2-positive ones [24].…”

Section: Parsleymentioning

confidence: 99%

Potential Therapeutic Effects of Phytochemicals and Medicinal Herbs for Cancer Prevention and Treatment

Jacobs¹

2018

Arch Gen Intern Med

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Cancer has a major impact on society across the world. It is one of the major causes of death, with approximately 1.7 million new cases expected to be diagnosed in the United States and 600,000 people will die from the disease. Worldwide statistics in 2012 reported over 14 million new cases and 8.2 million cancer-related deaths. New cases are expected to rise by about 70% over the next 20 years. The current standard of care, surgery, chemotherapy, and radiation has not proved to be highly effective and comes with significant side effects and costs. Although conventional cancer therapy can target certain cancer cells and sometimes prevent relapse of the illness, complementary and alternative therapies are required due to disadvantages of the current therapies such as low effectiveness, adverse effects, or availability. Moreover, a number of studies have found adjunctive therapeutic approaches to improve outcomes for cancer patients. One potential complementary method with conventional cancer drugs involves the use of medicinal herbs and phytochemicals. The possible therapeutic benefits include, but are not limited to antiproliferative, apoptotic, anti-metastatic, and anti-angiogenic effects, of which have been demonstrated in in vitro experiments and some clinical trials; more clinical trials need to be done. This review summarizes some plant sources exhibiting anti-cancer and chemoprevention activity. An overview of how phytochemicals and medicinal herbs permit the prevention and ultimate removal of cancer cells with minimal trauma to the host will be presented.

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Antiproliferative and apoptotic effects of silibinin in rat prostate cancer cells

Cited by 81 publications

References 22 publications

Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells

Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells

Unique natural antioxidants (NAOs) and derived purified components inhibit cell cycle progression by downregulation of ppRb and E2F in human PC3 prostate cancer cells

Potential Therapeutic Effects of Phytochemicals and Medicinal Herbs for Cancer Prevention and Treatment

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