Antiproliferative and Apoptotic Effects ofSesbania grandifloraLeaves in Human Cancer Cells

Abstract

Natural phytochemicals and their derivatives are good drug candidates for anticancer therapeutic approaches against multiple targets. We report here the initial findings from our studies on the anticancer properties of the leaves of the medicinal plant Sesbania grandiflora. In the current study, five different solvent fractions from the leaves of S. grandiflora were tested on cancer cell lines such as MCF-7, HepG2, Hep-2, HCT-15, and A549. The methanolic fraction of S. grandiflora was found to exert potent antiproliferative effects especially in the human lung cancer cell line, A549. Caspase 3 was activated in the methanolic fraction treated A549 cells thereby leading to cell death by apoptosis. DAPI staining, DNA laddering, and decrease in mitochondrial membrane potential further confirmed the apoptotic mode of cell death. The high levels of ROS intermediates as evidenced by DCF-DA staining could have played a role in the apoptotic induction. Decrease in levels of cyclin D1 and decrease in the activation of NFkB were observed in A549 cells on treatment with methanolic fraction, giving a hint on the possible mechanism of action. These results prove that the medicinal plant S. grandiflora can be explored further for promising candidate molecules to combat cancer, especially lung cancer.

Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from Cassia fistula L. Leaves

“…The mitochondrial membrane potential (∆Ψm) of the fraction was analyzed as described by the method of Pajaniradje et al [21]. The MG-63 cells at a density of 5 × 10 5 cells/well were seeded in six-well plates for 24 h. After adherence, the cells were treated with various concentrations of the CFL1 for 24 h. After treatment, the media was discarded, and the cells were washed with PBS.…”

Apoptosis induction by Pleurotus sajor-caju (Fr.) Singer extracts on colorectal cancer cell lines

“…The pellet obtained was washed once with PBS, stained with AO/EB solution with 25 μL PBS and 2 μL AO/EB dye, incubated for 5 min, and observed under fluorescence microscope (BX43 -Olympus). Morphological changes were determined according to Pajaniradje et al (2014).…”

Comparación del cultivo celular de HeLa y HEp-2: Perspectivas de estudios con Chlamydia trachomatis

“…El medio DMEM es un medio que no contiene L-Glutamina (28) al contrario del medio F12 el cual contiene 146 mg/L de glutamina (23), por lo cual se le adicionó de manera externa, determinando que la concentracion 0,1% es la ideal de Glutamina , ya que esta genera un crecimiento más continuo obteniendo una confluencia más alta, entre el 50% en las primeras horas y hasta un 93% al terminar el cultivo, Figura 3A. Ensayos con concentraciones menores de L-Glutamina (29), no generaron la confluencia deseada en un tiempo estimado de 3 días, Figura 3B, para así posterior a esto poder realizar la infección con CT.…”