Antiproliferative and leishmanicidal effect of ajoene on various Leishmania species: ultrastructural study

Ledezma, Eliades; Jorquera, Alicia; Bendezú, Herminia; Vivas, Julio; Pérez, Gladinex

doi:10.1007/s00436-002-0649-9

Cited by 22 publications

(19 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taken together, these results strongly support the potential of garlic extracts for the immunotherapy of Leishmania infection. This immunomodulatory activity of garlic extract may also be complemented by the direct inhibitory effect of some garlic compounds such as ajoene, which can significantly inhibit Leishmania growth in vitro [32].…”

Section: Discussionmentioning

confidence: 99%

In vivo and In vitro Control of Leishmania mexicana due to Garlic‐induced NO Production

et al. 2007

View full text Add to dashboard Cite

Leishmania mexicana is the main causal agent of cutaneous leishmaniasis in the Yucatán peninsula in Mexico. Control of this disease is associated with a Th1‐type immune response and garlic extract has been reported as a Th1 immunomodulator in BALB/c mice infected with Leishmania major. In this study, we investigated the effect of garlic extracts on L. mexicana infection in vivo and in vitro. Garlic extract reduced footpad lesions in L. mexicana‐infected BALB/c mice by inducing IFN‐γ production from T cells. In vitro, garlic extract reduced macrophage infection through induction of nitric oxide (NO) production. Garlic extract may thus act on both T cells and macrophages to stimulate IFN‐γ production and NO synthesis for parasite killing. A 10‐ to 14‐kDa fraction was identified as responsible for the in vitro effect of the whole extract and may lead to the identification of novel immunomodulating drugs and therapeutic alternatives for the treatment of leishmaniasis.

show abstract

Section: Discussionmentioning

confidence: 99%

In vivo and In vitro Control of Leishmania mexicana due to Garlic‐induced NO Production

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Ajoene decreased intracellular L. mexicana infection by 40% at 10 µM, by 95% at 50 µM and by 100% at 100 µM while not affecting host macrophages. Ultrastructural studies showed a time-and dose-dependent morphological alteration of the mitochondrial membrane and nuclear envelope and the formation of large autophagic vacuoles and megasomes [160]. Ajoene (161) also induced cell lysis of T. cruzi epimastigotes at 100 µM.…”

Section: Miscellaneous Plant Extractsmentioning

confidence: 98%

Natural Products from Plants as Drug Candidates and Lead Compounds Against Leishmaniasis and Trypanosomiasis

Salem

Werbovetz²

2006

CMC

132

View full text Add to dashboard Cite

Millions of people in the developing world are affected by diseases caused by the kinetoplastid parasites: the leishmaniases, African trypanosomiasis, and Chagas disease. In many cases the drugs employed for treatment are toxic, marginally effective, given by injection, and/or compromised by the development of resistance. Since safe, effective, and affordable chemotherapeutic agents for leishmaniasis and trypanosomiasis are clearly needed, the identification of new antikinetoplastid drug candidates should be an urgent priority. Numerous plant-derived natural products from different structural classes have been investigated as antileishmanial and antitrypanosomal candidates, including various alkaloids, terpenoids, flavonoids, and quinonoids. This review outlines the antikinetoplastid activities of plant-derived natural products reported in the literature and also provides an overview of mechanistic studies that have been conducted with these compounds. Given the activities of these agents and their diverse range of effects on parasite biology, natural products are a potentially rich source of drug candidates and leads against leishmaniasis and trypanosomiasis.

show abstract

“…Earlier, ajoene was found to be a potent inhibitor of Leishmania mexicana and Leishmania venezuelensis but at IC 50 2.0-4.5 μg/mL. [28] . Other studies reported that the susceptibility of amastigotes of Leishmania mexicana to pentostam and paromomycin was at concentrations of 4.1-21 and 24 μg/mL, respectively.…”

Section: Discussionmentioning

confidence: 98%

“…The results of current study revealed significant axenic anti‐amastigote and antipromastigote activity of hirudin with IC 50 values considerably lower than glucantime. Earlier, ajoene was found to be a potent inhibitor of Leishmania mexicana and Leishmania venezuelensis but at IC 50 2.0–4.5 μg/mL …”

Section: Discussionmentioning

confidence: 99%

In‐vitroantileishmanial potential of peptide drug hirudin

et al. 2016

View full text Add to dashboard Cite

Hirudin is clinically an important drug used for the treatment of cardiac diseases, but has never been elucidated for antileishmanial potential. This study was designed to determine the therapeutic utility of hirudin against leishmaniasis. Binding affinities of 28 potent proteinase inhibitors were screened computationally against leishmanolysin (GP63), out of which hirudin exhibited higher binding affinity with GP63 and good expected IC values. Experimentally, hirudin showed most promising activity against promastigote and axenic amastigote forms of leishmanial parasites with IC values of 0.60 ± 0.36 μg/mL and 0.43 ± 0.23 μg/mL, respectively, in a dose- and time-dependent assay. The cytotoxicity assay revealed no adverse effects on human macrophages with LD value of 860.11 ± 53.44 μg/mL. Hirudin caused leishmanial cell death mainly by apoptosis and membrane permeability. In spite of the basic knowledge obtained, hirudin mechanism is considerably less prone to the induction of resistance than classical drugs. Collectively, this study fosters further studies for the hirudin as new antileishmania lead with a new mode of action.

show abstract

Antiproliferative and leishmanicidal effect of ajoene on various Leishmania species: ultrastructural study

Cited by 22 publications

References 8 publications

In vivo and In vitro Control of Leishmania mexicana due to Garlic‐induced NO Production

In vivo and In vitro Control of Leishmania mexicana due to Garlic‐induced NO Production

Natural Products from Plants as Drug Candidates and Lead Compounds Against Leishmaniasis and Trypanosomiasis

In‐vitroantileishmanial potential of peptide drug hirudin

Contact Info

Product

Resources

About