Antiproliferative and phytochemical analyses of leaf extracts of ten Apocynaceae species

Wong, Siu Kuin; Lim, Yau Yan; Abdullah, Noor Rain; Nordin, Fariza Juliana

doi:10.4103/0974-8490.81957

Cited by 43 publications

(28 citation statements)

References 26 publications

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“…Furthermore, it has been reported that crude aqueous and methanolic extracts of Vernonia guineensis (Asteraceae) tuber demonstrates relatively weaker activity against DU145 cells, whereas dichloromethane extract is more potent with lower IC50 values (Toyang et al, 2012). Likewise, Wong et al (2011b) stated that only DCM (dichloromethane : methanol) leaf extract of Catharanthus roseus showed activity against MCF-7 cells while hexane, methanol, DCM and dichloromethane extracts were all found to be cytostatic (anti-proliferative). Considering the results of our study and the mentioned studies above, it can be said that activities of A. orientalis extracts may be augmented using different solvents in the extraction process.…”

Section: Discussionmentioning

confidence: 99%

Cytostatic Effects of Methanolic Extracts of Amsonia orientalis Decne. on MCF-7 and DU145 Cancer Cell Lines

Acemi

Duruksu

Özen

2017

Not Bot Horti Agrobo

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Amsonia orientalis is an ornamental plant with anti-cancer properties. To characterize the anti-cancer potential of the species, methanolic extracts were prepared from leaf and stem tissues of field-grown individuals. Also, a mixture of stems, leaves and roots of in vitro grown plantlets (SLR) were extracted with methanol and used. Effects of these extracts on the cancer cell lines MCF-7 (breast) and DU145 (prostate) were investigated. After incubation for 48 h, the leaf extract gave the lowest IC 50 values (197.8 µM for MCF-7 and 300.9 µM for DU145). The SLR extract also gave the similar results, but with higher IC 50 values. The stem extract had the weakest effect on cancer-cell-specific cytotoxicity. The extracts did not have a significant effect on DU145, compared to fibroblasts. The leaf extract increased the number of active-Caspase-3 positive cells in MCF-7 culture slightly, but the main effect of all extracts was on the cell proliferation rather than apoptosis. Real-time cellular analysis of MCF-7 demonstrated a 1.8-fold increase of doubling time for the leaf extract. The expression of proliferation-related gene MCM2 was found to be decreased in MCF-7 cells treated with the leaf extract, while its expression increased in fibroblasts after the same treatment. In conclusion, the extracts affected the MCF-7 cells at moderate concentrations. This study suggests that the leaf methanolic extracts might be a good source of compounds with cytostatical properties.

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Section: Discussionmentioning

confidence: 99%

Cytostatic Effects of Methanolic Extracts of Amsonia orientalis Decne. on MCF-7 and DU145 Cancer Cell Lines

Acemi

Duruksu

Özen

2017

Not Bot Horti Agrobo

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“…Bark and latex are used as diuretic and purgative. [12] The essential oil obtained from the flower is used as ingredient in cosmetics, candles, potpourri, massage oils and aromatherapy.…”

Section: Ethnobotanical Usesmentioning

confidence: 99%

Comprehensive Overview of Plumeria Obtusa

Narwariya¹

2017

WJPR

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The various species of Plumeria are known to have medicinal properties and have a long history of use by indigenous and tribal people in India. The medicinal value of this Plumeria species in the treatment of a large number of human ailments is mentioned in Ayurveda, Charaka Samhita and Sushruta Samhita mentions the medicinal value of Plumeria species in the treatment of a large number of human diseases. These plants are well known for their religious value, cosmetic importance and tremendous potential to be used as medicinal agents to cure infections, digestive diseases, antiinflammatory and antipyretic action, anti-tumor potential, anti-oxidant properties, as a purgative, remedy for diarrhea, bronchitis, cough, asthma, fever bleeding piles, dysentery, blood disorders and tumors etc. Today it is a challenge for scientists to provide efficient, safe and cheap medications. In this case Plumeria can be an exclusive medicine which is widely distributed throughout our country. The present review gives an overview of P.obtusa with reference to to its botanical aspects, ethnobotanical uses, Phytochemistry and pharmacological activity.

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“…[45] Allamanda cathartica, Cerbera odollam, Dyera costulata , and Kopsia fruticosa did not display any APF activity. Leaves were sequentially extracted with hexane (Hex), dichloromethane (DCM), and methanol (MeOH).…”

Section: Introductionmentioning

confidence: 99%

Caffeoylquinic acids in leaves of selected Apocynaceae species: Their isolation and content

Wong¹,

Lim²,

Ling³

et al. 2014

Phcog Res

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Background:Three compounds isolated from the methanol (MeOH) leaf extract of Vallaris glabra (Apocynaceae) were those of caffeoylquinic acids (CQAs). This prompted a quantitative analysis of their contents in leaves of V. glabra in comparison with those of five other Apocynaceae species (Alstonia angustiloba, Dyera costulata, Kopsia fruticosa, Nerium oleander, and Plumeria obtusa), including flowers of Lonicera japonica (Japanese honeysuckle), the commercial source of chlorogenic acid (CGA).Materials and Methods:Compound were isolated by column chromatography, and identified by NMR and MS analyses. CQA content of leaf extracts was determined using reversed-phase HPLC.Results:From the MeOH leaf extract of V. glabra, 3-CQA, 4-CQA, and 5-CQA or CGA were isolated. Content of 5-CQA of V. glabra was two times higher than flowers of L. japonica, while 3-CQA and 4-CQA content was 16 times higher.Conclusion:With much higher CQA content than the commercial source, leaves of V. glabra can serve as a promising alternative source.

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Antiproliferative and phytochemical analyses of leaf extracts of ten Apocynaceae species

Cited by 43 publications

References 26 publications

Cytostatic Effects of Methanolic Extracts of Amsonia orientalis Decne. on MCF-7 and DU145 Cancer Cell Lines

Cytostatic Effects of Methanolic Extracts of Amsonia orientalis Decne. on MCF-7 and DU145 Cancer Cell Lines

Comprehensive Overview of Plumeria Obtusa

Caffeoylquinic acids in leaves of selected Apocynaceae species: Their isolation and content

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