Antiproliferative, Antimicrobial and Apoptosis Inducing Effects of Compounds Isolated from Inula viscosa

Abstract

Abstract:The antiproliferative and antimicrobial effects of thirteen compounds isolated from Inula viscosa (L.) were tested in this study. The antiproliferative activity was tested against three cell lines using the MTT assay. The microdilution method was used to study the antimicrobial activity against two Gram positive bacteria, two Gram negative bacteria and one fungus. The apoptotic activity was determined using a TUNEL colorimetric assay. Scanning electron microscopy was used to study the morphological changes in treated cancer cells and bacteria. Antiproliferative activity was observed in four flavonoids (nepetin, 3,3′-di-O-methylquercetin, hispidulin, and 3-O-methylquercetin). 3,3′-di-OMethylquercetin and 3-O-methylquercetin showed selective antiproliferative activity against MCF-7 cells, with IC 50 values of 10.11 and 11.23 µg/mL, respectively. Both compounds exert their antiproliferative effect by inducing apoptosis as indicted by the presence of DNA fragmentation, nuclear condensation, and formation of apoptotic bodies in treated cancer cells. The antimicrobial effect of Inula viscosa were also noticed in 3,3′-di-O-methylquercetin and 3-O-methyquercetin that inhibited Bacillus cereus at MIC of 62.5 and 125 µg/mL, respectively. Salmonella typhimurium was inhibited by both compounds at MIC of 125 µg/mL. 3,3′-di-O-Methylquercetin induced damage in bacterial cell walls and cytoplasmic membranes. Methylated quercetins isolated from Inula viscosa have improved anticancer and antimicrobial properties compared with other flavonoids and are promising as potential anticancer and antimicrobial agents.

Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity

“…Even though there are precedents in the literature about the antibacterial activity exerted by compound 2 against E. coli and against some methicillin-susceptible and methicillin-resistant S. aureus (Hirai et al 2010;Kuete et al 2011), other authors reported a lack of effectiveness (Hossion et al 2010;Li et al 2009) matching that obtained in this work. The level of activity shown by compound 3 against E. coli and S. aureus, does not match that found by Van Puyvelde et al (1989), but it does agree with the results obtained by Talib et al (2012) in a microtiter plate dilution test performed against E. coli. Discrepancies in the effectiveness of flavonoids have been attributed to differences in the solubility of these compounds, in the conditions of the assays performed and in the judgment of the MICs (Cushnie and Lamb 2005;Wu et al 2008).…”

1 H NMR based metabolic profiling of eleven Algerian aromatic plants and evaluation of their antioxidant and cytotoxic properties

“…The choice of our investigated plants is based on two criteria: first, in this domain there is no study in Algeria that deals with these plants, and the second criterion is that these plants have ethnopharmacological data indicating their traditional utilization in the treatment of different diseases and disorders (Paula de Oliveira et al, 2011). In addition, some of them have been proved to be efficient in the treatment of various cancerous lesions (Xavier, Lima, Fernandes-Ferreira, & Pereira-Wilson, 2009;Aissaoui, Zizi, Israili, & Lyoussi, 2011;Sertel, Eichhorn, Plinkert, & Efferth, 2011;Talib, Zarga, & Mahasneh, 2012) and as potential neuroprotectants (Vora, Patil, & Pillai, 2009;Kelsey, Wilkins, & Linseman, 2010;Pacifico et al, 2013;Pacifico et al, 2014). Due to their traditional utilization and active components, these plants are also considered to be efficient for the treatment of free radical-related disorders.…”

The use of medicinal herbs in gynecological and pregnancy-related disorders by Jordanian women: a review of folkloric practice vs. evidence-based pharmacology

“…Selecive antiproliferative and proapoptotic capacity in breast cancer cells; cytotoxic and genotoxic effect in A. cepa. Hypoglycemic properties via stimulation of glucose uptake Afifi-Yazar et al 2011;Talib et al 2012;Seca et al 2014). Antimicrobial and anti-Helicobacter pylori efficacies (Karygianni et al 2014;Masadeh et al 2014).…”

“…Antifeedant activity of its pyrrolizidine alkaloids (Sicilliano et al 2005). Antidiabetic activity of its aqueous extract in STZ-diabetic rats (Muhammed & Ari 2012;Al-Snafi 2014 Talib et al 2012); chlorogenic-and caffeic acids, rutin, myricetin, quercetin, luteolin and kaempferol (Gökbulut et al 2013); bi-and tri-cyclic sesquiterpene lactones; inuloxins A-D (Andolfi et al 2013). …”