Antiproliferative, Antioxidant, and Antimutagenic Activities of Flavonoid-Enriched Extracts from (Tunisian)Rhamnus alaternusL.: Combination with the Phytochemical Composition

Abstract

A pronounced antiproliferative effect on human leukemia K562 cells was shown with flavonoid-enriched extracts from Rhamnus alaternus roots and leaves, with, respectively, IC(50) values of 165 and 210.73 microg/mL. High DPPH radical-scavenging activity (7.21 and 18.84 microg/mL, respectively) and antioxidative effects using the xanthine oxidase assay (IC(50) values of 83.33 and 103.96 microg/mL, respectively) were detected in the presence of the two tested extracts. Although no mutagenic effect was observed when using the Salmonella typhimurium assay system with TA1535 and TA100 strains, the two tested extracts exhibited a high-level protection toward the direct mutagen, sodium azide-induced response.

Antioxidant and free radical-scavenging properties of three flavonoids isolated from the leaves of Rhamnus alaternus L. (Rhamnaceae) : A structure-activity relationship study

“…It has traditionally been used as a digestive, diuretic, laxative, hypotensive and for the treatment of hepatic and dermatological complications (Boukef, 2001). Previous studies have shown potent antioxidant, free radical scavenging, antimutagenic and antigenotoxic activities of crude extracts from R. alaternus (Chevolleau, Debal, & Ucciani, 1992;Ben Ammar et al, 2005, 2007a, 2008a, 2008b. We have also reported others biological activities of R. alaternus extracts: antibacterial and antiproliferative.…”

Genotoxic and antigenotoxic effects of Hemidesmus indicus R. Br. root extract in cultured lymphocytes

“…The antiproliferative property of Hemidesmus indicus extract has been reported by earlier investigators (Thabrew et al, 2005). Both coumarins and flavonoids have been attributed to possess antiproliferative properties (Kostova, 2005;Ammar et al, 2008) and may be responsible for the cytotoxic effects exhibited by the root extract of H. indicus.…”

“…The high phenolic content of some herbs consumed by Thai people was linked to their antimutagenic potential (Kruawan and Kangsadalampai, 2006). Recently, flavonoid-enriched extracts from Rhamnus alaternus roots and leaves were shown to demonstrate significant protection against sodium azide (Ammar et al, 2008).…”

Rhamnus alaternus Plant: Extraction of Bioactive Fractions and Evaluation of Their Pharmacological and Phytochemical Properties

“…The Rhamnus alaternus plant, the so-called "Imlilesse or Safir" in the North of Algeria, has been traditionally used for a long time in various medicine areas as infusion notably for its gastric, hypotensive, purgative, laxative, diuretic, antihypertensive, hepatoprotective, and digestive effects and finally to treat dermatological complications [20]. Such biological activities would be related to the natural presence of beneficial compounds as evidenced by many experimental studies, that pointed out that R. alaternus contains important metabolites-such as flavonoids, coumarins, glycosides, tannins, anthraquinones, and polyphenolic compounds [21,22]. Some of these molecules were isolated from Rhamnus alaternus using various extraction processes (i.e., maceration, decoction, hydrodistil-lation, soxhlet, ultrasonic extraction) and demonstrated various pharmacological properties including antihyperlipidemic, antioxidant, antigenotoxic, antiproliferative, and antimutagenic activities.…”

“…Some of these molecules were isolated from Rhamnus alaternus using various extraction processes (i.e., maceration, decoction, hydrodistil-lation, soxhlet, ultrasonic extraction) and demonstrated various pharmacological properties including antihyperlipidemic, antioxidant, antigenotoxic, antiproliferative, and antimutagenic activities. Some biological activities, especially antibacterial and antiproliferative effects were reviewed elsewhere [8,20,[22][23][24].…”