Antiproliferative effect of Dendrobium catenatum Lindley polypeptides against human liver, gastric and breast cancer cell lines

Abstract

Dendrobium catenatum Lindley is a precious plant with both dietary and medicinal applications. However, the antiproliferative activity of D. catenatum-derived peptides has not been investigated. In this study, the whole proteins of D. catenatum were extracted, hydrolysis with three proteases (alcalase 2.4L, alcalase 37017 and trypsin) was performed, and gel filtration chromatography was employed to obtain nine fractions. Fraction A3 possessed the best antiproliferative activity in vitro, with percentage inhibitions of 73.38%, 78.91% and 86.8% against HepG-2, SGC-7901 and MCF-7 cancer cells, respectively, and an inhibition of only 5.52% against L-O2 normal liver cells at 500 μg mL(-1). Subsequently, mass spectrometry revealed the existence of 10 alcalase-derived peptides in fraction A3, and the sequences of the three most abundant peptides were determined by de novo sequencing as: RHPFDGPLLPPGD, RCGVNAFLPKSYLVHFGWKLLFHFD and KPEEVGGAGDRWTC. Moreover, these peptides were synthesized and their antiproliferative activities in vitro were also confirmed. This suggests that fraction A3 may be promising for use in food and pharmaceutical applications.

Bioactive Peptides

“…These peptides have been reported to exert strong antiproliferative effects on colorectal cancer HT-29 cells. The peptides RHPFDGPLLPPGD, RCGVNAFLPKSYLVHFGWKLLFHFD and KPEEVGGAGDRWTC obtained from Dendrobium catenatum Lindley demonstrated strong antiproliferative activity against HepG-2, SGC-7901 and MCF-7 cancer cells [148]. Peptides from rapeseed protein fermentates have also been shown to inhibit the proliferation of human HepG2 liver cancer, human MCF-7 breast cancer and human MCF-7 breast cancer cell lines [149].…”

Separation and nanoencapsulation of antitumor peptides from Chinese three-striped box turtle (Cuora trifasciata)

“…Mass spectrometry analysis and peptide identification were performed as described in Zheng et al (2015). Briefly, matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) (UltraFleXtreme, Bruker, Karlsruhe, Germany) was performed in a positive ion reflectron mode to obtain MS spectra.…”

Dendrobium: Sources of Active Ingredients to Treat Age-Related Pathologies

“…However, when testing the D. speciosum stems extract for anti-genotoxicity, low doses result in enhanced activity, whereas higher doses result in no effect. Anti-proliferative effects were assigned to alcalase-derived polypeptides of Dendrobium catenatum against human liver (HepG2), gastric (SGC-7901) and breast (MCF-7) cancer cell lines [ 26 ]. The best anti-proliferative activity in vitro with a percentage of inhibitions of 73.38%, 78.91% and 86.8% against HepG2, SGC-7901 and MCF-7 cancer cells, respectively, were obtained at 500 µg/mL.…”