Antiproliferative effect of Ficus carica latex on cancer cell lines is not solely linked to peroxidase-like activity of ficin

Boyacioglu, Olcay; Kara, Betul; Tecimen, Seçil; Kılıç, Merve; Delibaş, Mehmet; R, Erdoğan; Ozdemir, Esra; Bahadır, Azad; Orenay-Boyacioglu, Seda

doi:10.1016/j.eujim.2021.101348

Cited by 15 publications

(8 citation statements)

References 14 publications

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“…On the contrary, F. carica latex extract showed no cytotoxic effect on human normal melanocyte cells (HFB4) in comparison to doxorubicin (86.20 ± 8.88), a known chemotherapeutic agent (Abdel-Aty et al, 2019). In human prostate (PC3) and colon (HT-29) cancer cell lines, good inhibition results were achieved, although it was suggested that ficin peroxidase-like activity was not sufficient to explain all the results (Boyacıoglu et al, 2021). Recently, F. carica extracts have been administered in vivo to suppress the side effects associated with the use of specific drugs in cancer therapies, including 5-fluorouracil and cisplatin, by taking advantage of its synergistic effect with extra virgin olive oil (Elghareeb et al, 2021).…”

Section: Anti-tumor and Immunomodulatory Propertiesmentioning

confidence: 97%

“…The Figs are an excellent source of different biologically active biomolecules and are responsible for a wide range of bioactive effects: antioxidant, anti-inflammatory, anticancer, antimicrobial, anti-ageing, and wound healing effects (Abdel-Rahman et al, 2021;Boyacıoglu et al, 2021). The main bioactive molecules present in various fig and fig by-products are terpenoids, carotenoids, phytosterols, volatile organic compounds, phenolic acids, and flavonoids, including anthocyanins, flavonols, flavan-3-ols, and flavanones (Pereira et al, 2017).…”

Section: Extraction Separation and Refinement Of Fig Biomoleculesmentioning

confidence: 99%

“…This juice is secreted in an appreciable amount, and its principal activity is the protection and self-healing of plants from external assaults. These diverse metabolites present in the latex are responsible for the biological properties of latex extracts, namely, antioxidant, cytotoxic and wound healing effects (Abdel-Aty et al, 2019;Boyacıoglu et al, 2021). Ficin (EC:3.4.22.3), a cysteine endopeptidase enzyme, represents an essential component of Ficus latex.…”

Section: Ficinmentioning

confidence: 99%

“…Latex has been highlighted as a potential inhibitor of cell proliferation (Abdel-Aty et al, 2019;AlGhalban et al, 2021;Boyacıoglu et al, 2021) (Table 3). The cytotoxic effect of F. carica leaf latex assessed in breast cancer cells (MDA-MB-231) showed a higher cytotoxic effect and involved different molecular mechanisms of action in comparison to F. salicifolia (AlGhalban et al, 2021).…”

Section: Anti-tumor and Immunomodulatory Propertiesmentioning

confidence: 99%

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Fig “Ficus carica L.” and its by-products: A decade evidence of their health-promoting benefits towards the development of novel food formulations

Ayuso

Carpena

Taofiq

et al. 2022

Trends in Food Science & Technology

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Section: Anti-tumor and Immunomodulatory Propertiesmentioning

confidence: 97%

Section: Extraction Separation and Refinement Of Fig Biomoleculesmentioning

confidence: 99%

Section: Ficinmentioning

confidence: 99%

Section: Anti-tumor and Immunomodulatory Propertiesmentioning

confidence: 99%

See 2 more Smart Citations

Fig “Ficus carica L.” and its by-products: A decade evidence of their health-promoting benefits towards the development of novel food formulations

Ayuso

Carpena

Taofiq

et al. 2022

Trends in Food Science & Technology

View full text Add to dashboard Cite

“…This juice is secreted in significant quantities, and its primary function is to protect and cure plants from external assaults. In addition, many latex metabolites are responsible for latex extracts' biological features, such as antioxidant, cytotoxic, and wound healing traits (Abdel‐Aty et al., 2019; Barolo et al., 2014; Boyacıoglu et al., 2021; Ramadan, 2023).…”

Section: Chemical Profile and Nutritional Value Of Fig (Ficus Carica)mentioning

confidence: 99%

Recent insight on nutritional value, active phytochemicals, and health‐enhancing characteristics of fig (Ficus craica)

Alzahrani,

Alshaikhi,

Hazzazi

et al. 2024

Food Safety and Health

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The current review highlights the nutritional values, phytochemical profile, and health‐enhancing characteristics of figs (Ficus carica L., family Moraceae). Ficus carica is one of the oldest fruit trees. The fruit has been appreciated as food and for its biomedical properties since ancient times. Ficus carica contains essential bioactive compounds that enhance health. The plant is characterized by unique compounds in all parts (peel, pulp, leaves, seed, and latex) besides primary nutrients (fatty acids, carbohydrates, vitamins, and minerals). Ficus carica is rich in bioactive secondary products such as flavonoids, furanocoumarin, anthocyanin, pectin, and phytosterols, contributing to many health benefits. Bioactive phytonutrients found in fig are used as antioxidant and antidiabetic agents due to the presence of specific functional groups that control diabetes. The health‐enhancing effects of fig, including anti‐tumor, anti‐inflammatory, and anti‐osteoporosis, are related to high levels of minerals essential to bone health and their benefits on Alzheimer's disease. All parts of Ficus carica have been used in cosmetics and pharmaceutical products, especially wound healing. In addition, Ficus carica has many uses in food items as a colorant and a preservative. To the best of knowledge, there is a lack of reports discussing the biochemistry, bioactivity, and medicinal traits of Ficus carica and the plant parts. This review gathered scientific information regarding the chemical profile, biological traits, and different uses of Ficus carica.

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Thermal Inactivation, Denaturation and Aggregation Processes of Papain‐Like Proteases

Koroleva,

Lavlinskaya,

Holyavka

et al. 2024

Chemistry & Biodiversity

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The investigation into the behavior of ficin, bromelain, papain under thermal conditions holds both theoretical and practical significance. The production processes of medicines and cosmetics often involve exposure to high temperatures, particularly during the final product sterilization phase. Hence, it's crucial to identify the "critical" temperatures for each component within the mixture for effective technological regulation. In light of this, the objective of this study was to examine the thermal inactivation, aggregation, and denaturation processes of three papain‐like proteases: ficin, bromelain, papain. To achieve this goal, the following experiments were conducted: (1) determination of the quantity of inactivated proteases using enzyme kinetics with BAPNA as a substrate; (2) differential scanning calorimetry (DSC); (3) assessment of protein aggregation using dynamic light scattering (DLS) and spectrophotometric analysis at 280 nm. Our findings suggest that the inactivation of ficin and papain exhibits single decay step which characterized by a rapid decline, then preservation of the same residual activity by enzyme stabilization. Only bromelain shows two steps with different kinetics. The molecular sizes of the active and inactive forms are similar across ficin, bromelain, and papain. Furthermore, the denaturation of these forms occurs at approximately the same rate and is accompanied by protein aggregation.

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Antiproliferative effect of Ficus carica latex on cancer cell lines is not solely linked to peroxidase-like activity of ficin

Cited by 15 publications

References 14 publications

Fig “Ficus carica L.” and its by-products: A decade evidence of their health-promoting benefits towards the development of novel food formulations

Fig “Ficus carica L.” and its by-products: A decade evidence of their health-promoting benefits towards the development of novel food formulations

Recent insight on nutritional value, active phytochemicals, and health‐enhancing characteristics of fig (Ficus craica)

Thermal Inactivation, Denaturation and Aggregation Processes of Papain‐Like Proteases

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