Antiproliferative effect of methanolic extract of Allophylus cobbe in C127I cell lines

Abstract

Scientific world is in search of newer and effective therapies against cancer and nature form a good source of drugs. The present study was undertaken to assess the antiproliferative potential of methanolic extract of A. cobbe in C127I cell lines. The leaves of A. cobbe were shade dried and was extracted using methanol and qualitative phytochemical analysis was performed. The extract was assessed for its cytotoxicity by MTT dye reduction assay in C127 I cells maintained using DMEM and 10 per cent foetal bovine serum at concentrations of 320, 160, 80, 40, 20, 20 and 5 µg/mL and the percent cell inhibition and IC50 were calculated. Acridine Orange/Ethidium bromide staining was used to detect the possible mechanism of cytotoxicity. From the results of MTT assay, it could be seen that there was a dose dependent inhibition of cell proliferation of C127I which was maximum at a concentration of 320 µg/mL. The IC50 value of the methanolic extracts was found to be 64.63 µg/mL respectively. The effect was comparable to doxorubicin. The extract and positive control treated cells showed orange to red fluorescence when stained with Acrdine Orange/ Ethidium bromide compared to greenish fluorescence in the control cells indicating apoptosis in the treated cells. The study concluded that methanolic extract of A. cobbe induced cytotoxicity by apoptosis of cancer cells.