Antiproliferative Effects of Alkaloids Isolated from the Tuber of <i>Stephania venosa via</i> the Induction of Cell Cycle Arrest in Mammalian Cancer Cell Lines

Abstract

Approach:The research was carried out to extract, isolate, purify and elucidate structure of the active compound from the tuber S. venosa. Most of the solvent extracts and isolated compound were evaluated with kinds of mammalian cancer cell lines for investigation on antiproliferative effects. Results: Four alkaloids, tetrahydropalmatine (1), crebanine (2) O-methylbulbocapnine (3) and Nmethyltetrahydropalmatine (4) were isolated from the tuber of S. venosa. Charaterization of the compounds were carried out by extensive NMR studies using COSY, HMQC, HMBC and DEPT in addition to other spectroscopic methods. These compounds (1, 2 and 3) were showed evidence of the anticancer activities for cell proliferation inhibition in K562, K562/Adr, GLC4 and GLC4/Adr cell lines due to G0/G1 obstruction by compound 2 and 3 and negligible S phase arrest by compound 1. Conclusion:The result showed slightly increase in S phase by the effect of compound 1, beside the G0/G1 phase was blocked by compound 2 and 3.

The effect of crebanine on memory and cognition impairment via the alpha-7 nicotinic acetylcholine receptor

“…Crebanine is an isoquinoline alkaloid found in several plants, including Stephania venosa, which is known in Thai as Sabuleud (Ingkaninan et al, 2006;Nantapap et al, 2010). The herbal extract from S. venosa has been widely used for a variety of indications in traditional Thai medicine (Likhitwitayawuid et al, 1993), yet the mechanism of therapeutic action of its alkaloids has not been well defined, especially in nerve tonic indications.…”

Antiinflammatory Activities of Crebanine by Inhibition of NF-κB and AP-1 Activation through Suppressing MAPKs and Akt Signaling in LPS-Induced RAW264.7 Macrophages

“…Crebanine was isolated and purified from ethyl acetate fraction of the tube of Stephania vernosa as our previously described. 15,18) It was obtained as light yellow crystals, melting point at 117.0-117.8°C. The structure of crebanine was elucidated by extensive spectroscopic analysis (UV, IR, MS, 1 H-NMR,…”

Alkaloids from <i>Stephania venosa</i> as Chemo-Sensitizers in SKOV3 Ovarian Cancer Cells <i>via</i> Akt/NF-κB Signaling

“…In recent years, CN has gained much attention due to its biological activities and proven pharmacological safety. 7,8) It was shown that CN more selectively decrease the survival and proliferation of cancerous cell lines than it does normal human fibroblasts.…”

“…In recent years, CN has gained much attention due to its biological activities and proven pharmacological safety. 7,8) It was shown that CN more selectively decrease the survival and proliferation of cancerous cell lines than it does normal human fibroblasts. 9) Moreover, CN has anti-invasive property in cancer cells by inhibiting constitutive nuclear factor kappa B (NF-κB) activation.…”

“…In recent years, CN has gained much attention due to its biological activities and proven pharmacological safety. 7,8) It was shown that CN more selectively decrease the survival and proliferation of cancerous cell lines than it does normal human fibroblasts.9) Moreover, CN has anti-invasive property in cancer cells by inhibiting constitutive nuclear factor kappa B (NF-κB) activation.10) On the other hand, THP has been stated to possess multi-drug resistant reversing properties in human breast cancer cells.11) These established results have led us to hypothesize that these natural alkaloids may possibly improve the effectiveness of the traditional anticancer drugs.Although the anti-cancer properties of CN have been studied in numerous cancer cell lines, it remains unidentified whether CN has chemo-sensitizing effect on drug-resistant cancer cells. Therefore, the purpose of the present study was to determine whether CN and its natural analogues show chemo-sensitizing effects in response to platinum drug in human…”