Antiproliferative evaluation of tall-oil docosanol and tetracosanol over CHO-K1 and human melanoma cells

Vergara, Mauricio; Olivares, Araceli; Altamirano, Claudia

doi:10.1016/j.ejbt.2015.05.004

Cited by 14 publications

(6 citation statements)

References 21 publications

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“…Other fatty alcohol derivatives such as 1-Eicosanol and lignoceric alcohol have not been reported to show cytotoxic effects on breast or prostate cancer. Nevertheless, they show antiproliferative activity for other cancer cell lines [57, 58]. Regarding the fatty acids and their derivative content in non-polar Ephedra extract, they could be related with activity and selectivity due to the n -tetra, n -penta, n -hexa, and n- octadecanoic acids present in the hexane extract corresponding to 41.37% of the total extract (see Table 7).…”

Section: Discussionmentioning

confidence: 99%

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

Mellado

Soto

Madrid

et al. 2019

BMC Complement Altern Med

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Background: Ephedra chilensis K Presl, known locally as pingo-pingo, is a Chilean endemic plant used in traditional medicine as an anti-inflammatory and used in other treatments. However, unlike for the other Ephedra species, there have been no reports on the antioxidant and cytotoxic effects of this plant. The present study aims to explore the potential applications of E. chilensis extract as a cytotoxic agent against in vitro cancer cell lines and to explore the relationship between this extract and antioxidant activity. Methods: Total anthraquinone, flavonoid, and phenolic contents, as well as antioxidant activity (DPPH, FRAP, and TRAP assays) and cytotoxic effect on several cancer cell lines (MCF-7, PC-3, DU-145, and HT-29) were measured for the hexane, dichloromethane and ethanol extracts of E. chilensis. In addition, several correlations among the phytochemical content, antioxidant activity, and cytotoxic effect were evaluated. Finally, GC-MS analyses of the most active extracts were carried out to identify their major components and to relate these components to the cytotoxic effect. Results: Antioxidant activity was found in the EtOH extracts of Ephedra, and the results were correlated with the phenolic content. For the cytotoxic activity, the non-polar extracts of E. chilensis had the highest antiproliferative effect for the MCF-7 and PC-3 cancer lines; the extract was shown to be up to three times more selective than doxorubicin. However, the cytotoxic effect was not correlated with the antioxidant activity. Lastly, the GC-MS analysis showed a high concentration of saturated fatty acids (mainly n-hexadecanoic acid) and terpenoids (mainly 4-(hydroxy-ethyl)-γbutanolactone). Conclusion: The cytotoxic activity and selectivity of the non-polar extracts of E. chilensis for the MCF-7 and PC-3 cell lines could be related to the terpenic compounds and fatty acids of the extracts or to the synergistic effect of all of the compounds in the extracts. These non-polar extracts can be used for the development of new drugs against breast and prostate cancer.

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Section: Discussionmentioning

confidence: 99%

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

Mellado

Soto

Madrid

et al. 2019

BMC Complement Altern Med

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“…The detected docosanol (C22-OH) is known by its antiproliferative effect of chinese hamster ovary cells K1 (CHO-K1) and human melanoma (CRL-1974TM) cell lines 38 . Investigations concerning the effects of long chain alcohols are often performed with long chain alcohols isolated from sugarcane where these compounds make up 0.10-0.30% of its mass 39 .…”

Section: Gc-ms Analysismentioning

confidence: 99%

Exploring Pavlova pinguis chemical diversity: a potentially novel source of high value compounds

Fernandes

Martel

Cordeiro

2020

Sci Rep

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In the Haptophyta Pavlova pinguis J. C. Green 16 only specific classes of compounds have been analyzed to assess its potential as food for larval hatcheries in aquaculture and as ecological biomarkers 13,17,18. For instance, Milke, et al. 11 and Parrish, et al. 12 assessed the ability of P. pinguis and other Pavlova species to sustain postlarval sea scallop growth focusing on its proximate, fatty acid and sterol composition. In this microalga the complete characterization of lipid components (simple and complex lipids) is still largely unexplored 6,9. Thus, in the present study the analysis of the lipophilic fraction of P. pinguis was performed in order to identify its lipophilic features before and after alkaline hydrolysis through gas chromatography-mass spectrometry (GC-MS) and evaluate its prospects for further improvement in bioactive compounds. Materials and Methods Growth and culture conditions. The haptophyta Pavlova pinguis (RCC 1539) was obtained from the Roscoff Culture Collection (RCC). The microalgal cultures were made by inoculating starter cultures into 1L of sterile f/2-Si medium with pH adjusted to 7.0 under 70 μmol m −2 s −1 light intensity with 16:8 h (light: dark cycles) at 25 °C. At the end of the logarithmic phase, the medium was centrifuged for 7 min. at 3720 g and the pellets washed. Microalgae growth was monitored daily with a Neubauer-improved counting chamber (Marienfield-Superior) and a light microscope (Olympus BX41) with a 40x magnification. The specific growth rate was determined as described in Fernandes, et al. 19 .

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“…The compound n -tetracosanol was isolated from leave extracts of Combretum microphyllum and it was found to have antimutagenic activity against Salmonella typhimurium TA98 at a low concentration of 5 μg/ml, and because mutations play a role in the pathogenesis and development of cancerous cells, it thus prevents the pathological process of cancer which can be caused by mutations ( Makhafola et al, 2017 ). Vergara et al (2015) conducted a study on the antiproliferative evaluation of tetracosanol over Chinese hamster ovary cells K1 (CHO-K1) and human melanoma cells and it was found that tetracosanol had no cytotoxic effect on the growth of CHO-K1 cells whereas, for human melanoma cells, it affected the cell density and inhibited growth by 58%. In this study, only Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

The Antibacterial, Antitumor Activities, and Bioactive Constituents’ Identification of Alectra sessiliflora Bacterial Endophytes

2022

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Due to increased antimicrobial resistance against current drugs, new alternatives are sought. Endophytic bacteria associated with medicinal plants are recognized as valuable sources of novel secondary metabolites possessing antimicrobial, antitumor, insecticidal, and antiviral activities. In this study, five bacterial endophytes were isolated and identified from the medicinal plant, Alectra sessiliflora, and their antibacterial and antitumor activities were investigated. In addition, the crude extracts of the endophytes were analyzed using gas chromatography (GC) coupled with time-of-flight mass spectrometry (TOF-MS). The identified bacterial endophytes belong to three genera viz Lysinibacillus, Peribacillus, and Bacillus, with the latter as the dominant genus with three species. Ethyl acetate extracts from the endophytes were used for antimicrobial activity against eleven pathogenic strains through minimum inhibitory concentration (MIC). The antitumor activity against the Hela cervical, Hek 293 kidney, and A549 lung carcinoma cells was determined by the MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay. Lysinibacillus sp. strain AS_1 exhibited broad antibacterial activity against the pathogenic strains with MIC values ranging from 4 to 8 mg/ml, while Bacillus sp. strain AS_3 displayed MIC of 0.25 mg/ml. Crude extracts of Lysinibacillus sp. strain AS_1, Peribacillus sp. strain AS_2, and Bacillus sp. strain AS_3 showed growth inhibition of more than 90% against all the cancer cell lines at a concentration of 1,000 μg/ml. Untargeted secondary metabolite profiling of the crude extracts revealed the presence of compounds with reported biological activity, such as antimicrobial, antioxidant, anti-inflammatory, antitumor, and antidiabetic properties. This study reported for the first time, bacterial endophytes associated with A. sessiliflora with antibacterial and antitumor activities.

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Antiproliferative evaluation of tall-oil docosanol and tetracosanol over CHO-K1 and human melanoma cells

Cited by 14 publications

References 21 publications

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

Exploring Pavlova pinguis chemical diversity: a potentially novel source of high value compounds

The Antibacterial, Antitumor Activities, and Bioactive Constituents’ Identification of Alectra sessiliflora Bacterial Endophytes

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