Antiproliferative, Proapoptotic, Antioxidant and Antimicrobial Effects of Sinapis nigra L. and Sinapis alba L. Extracts

Boscaro, Valentina; Boffa, Luisa; Binello, Arianna; Amisano, Gabriella; Fornasero, Stefania; Cravotto, Giancarlo; Gallicchio, Margherita

doi:10.20944/preprints201810.0509.v1

Cited by 8 publications

(1 citation statement)

References 37 publications

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“…The high consumption level of Sinapis herb reduces the risk of a great number of cancers, particularly cancers of the gastrointestinal tract, lung, bladder and prostate (Johnson, 2018). The ability of Sinapis species to reduce health risks depends mostly on the biochemical quality of a plant (Boscaro et al, 2018). Therefore, the present study aims to examine the effect of Si application on concentrations and distribution of K + , Ca 2+ , Na + and P in leaves, stems, and roots of Sinapis nigra L. in saline conditions.…”

Section: Introductionmentioning

confidence: 99%

The accumulation of K+, Ca2+, Na+ and P in Sinapis nigra L. grown in the presence of NaCl and silicon

Daničić

Putnik-Delić

Lazic

et al. 2019

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Saline stress is recognized as one of the most important abiotic stresses for plants in some regions of the world. Recent studies showed that silicon (Si) can play an important role in plant resistance to environmental conditions, such as excessive salinity. In the present experiment, Sinapis nigra L. was exposed to two levels of salinity (50 and 100 mM NaCl). To study the effect of Si addition on the concentrations of K + , Ca 2+ , Na + , and P, in the vegetative parts of plants, 5 mM of Si in the form of fertilizer (Siliplant) was added to the nutrient solutions. The experiment was conducted in semi-controlled conditions. The results showed that both salinity levels significantly increased the concentration of Na + in leaves, stems, and roots, whereas the concentration of Ca 2+ and K + were decreased (up to 55% and 90% in the presence of 100 mM NaCl). The addition of Siliplant to the saline nutrient solution (50 mM NaCl) was efficient in decreasing Na + accumulation in leaves and stem, while in roots more alleviating effect of Si addition was pronounced in the presence of 100 mM NaCl (Na + concentration decreased by 50%). The presence of Si in 50 mM NaCl containing nutrient solution enhanced the accumulation of K + in leaves and stems, while Ca 2+ concentration was decreased, or the addition of Si to a nutrient solution had a negligible effect. Based on the results of the present study, the application of Si in the form of fertilizer was found more efficient in the presence of lower salt concentration (50 mM NaCl). Therefore, higher doses of the fertilizer should be considered and tested in case of higher NaCl concentrations in the substrate.

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Section: Introductionmentioning

confidence: 99%

The accumulation of K+, Ca2+, Na+ and P in Sinapis nigra L. grown in the presence of NaCl and silicon

Daničić

Putnik-Delić

Lazic

et al. 2019

Zb Mat srp prir nauk

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Biological Properties of Bee Bread Collected from Apiaries Located across Greece

et al. 2021

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Bee bread is the only fermented product of the beehive. It constitutes the main source of proteins, lipids, vitamins, and macro- and microelements in honeybee nutrition and it exerts antioxidant and antimicrobial properties, though research on these aspects has been limited so far. In this study 18 samples of Greek bee bread, two of which were monofloral, were collected during different seasons from diverse locations such as Crete and Mount Athos and were tested for their bioactivity. Samples were analyzed for their antibacterial properties, antioxidant activity, total phenolic content (TPC), and total flavonoid content (TFC). The antimicrobial activity of each sample was tested against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Salmonella typhimurium. Our data demonstrate that all samples exert inhibitory and most of them bactericidal activity against at least two pathogens. Furthermore, all samples exert significant antioxidant activity, where the monofloral Castanea Sativa sample demonstrated superior antioxidant activity. Nevertheless, the antioxidant and antimicrobial activity were not strongly correlated. Furthermore, machine learning methods demonstrated that the palynological composition of the samples is a good predictor of their TPC and ABTS activity. This is the first study that focuses on the biological properties of Greek bee bread and demonstrates that bee bread can be considered a functional food and a possible source of novel antimicrobial compounds.

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Exposure to Salinity and Light Spectra Regulates Glucosinolates, Phenolics, and Antioxidant Capacity of Brassica carinata L. Microgreens

et al. 2021

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The effect of salt treatment on Brassica carinata (BC) microgreens grown under different light wavelengths on glucosinolates (GLs) and phenolic compounds were evaluated. Quantifiable GLs were identified using ultra-high performance-quadrupole time of flight mass spectrometry. Extracts’ ability to activate antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) was evaluated on human colorectal carcinoma cells (HCT116). Furthermore, BC compounds’ ability to activate expression of nuclear transcription factor-erythroid 2 related factor (Nrf2) and heme-oxygenase-1 (HO-1) proteins was examined using specific antibodies on HCT116 cells. Sinigrin (SIN) was the abundant GLs of the six compounds identified and its content together with total aliphatic GLs increased in saline conditions. Fluorescent (FL) and blue plus red (B1R1) lights were identified as stable cultivation conditions for microgreens, promoting biomass and glucobrassicin contents, whereas other identified individual and total indole GLs behaved differently in saline and non-saline environments. Blue light-emitting diodes and FL light in saline treatments mostly enhanced SIN, phenolics and antioxidant activities. The increased SOD and CAT activities render the BC microgreens suitable for lowering oxidative stress. Additionally, activation of Nrf2, and HO-1 protein expression by the GLs rich extracts, demonstrate their potential to treat and prevent oxidative stress and inflammatory disorders. Therefore, effective salt treatments and light exposure to BC microgreens present an opportunity for targeted regulation of growth and accumulation of bioactive metabolites.

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Antiproliferative, Proapoptotic, Antioxidant and Antimicrobial Effects of Sinapis nigra L. and Sinapis alba L. Extracts

Cited by 8 publications

References 37 publications

The accumulation of K+, Ca2+, Na+ and P in Sinapis nigra L. grown in the presence of NaCl and silicon

The accumulation of K+, Ca2+, Na+ and P in Sinapis nigra L. grown in the presence of NaCl and silicon

Biological Properties of Bee Bread Collected from Apiaries Located across Greece

Exposure to Salinity and Light Spectra Regulates Glucosinolates, Phenolics, and Antioxidant Capacity of Brassica carinata L. Microgreens

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