Antiprothrombin antibodies detected in two different assay systems

Abstract

We evaluated the clinical significance of aPT and aPS-PT by testing for the presence of these antibodies in 212 SLE patients and in 100 healthy individuals. Results show that anti-prothrombin antibodies were found in 47% of the patients (aPT in 31% and aPS-PT in 31%). Their presence did not correlate with that of aCL, anti-beta2GPI, LA and/or anti-protein S. IgG but not IgM aPT were more frequently found in patients with thrombosis than in those without. IgG and IgM aPS-PT were also more frequent in patients with thrombosis (venous and/or arterial) than in those without. Levels of IgG aPT and IgG and IgM aPS-PT were higher in patients with thrombosis than in those without. Although aPT and aPS-PT were more frequently found in women with adverse obstetric history than in those without, the differences were not statistically significant. More significantly, 48% of the patients with aPL-related clinical features who were negative for standard tests had antiprothrombin antibodies. We can conclude that aPT and aPS-PT are frequently found in SLE. Their presence is associated with thrombosis, making these antibodies potential markers for the APS. Testing for these antibodies could be of clinical benefit in patients who are negative for the routinely used tests.

Comparative analysis of different enzyme immunoassays for assessment of phosphatidylserine-dependent antiprothrombin antibodies

“…The good correlation between aPS/PT ELISA and LA supports the use of aPS/PT as one of the "screening" or "confirming" assays for APS-associated LA [11,13]. Moreover, aPS/PT are associated with the clinical manifestations of APS [11,14,15] and the determination of aPS/PT would potentially contribute to a better recognition of APS, especially in cases of suspected APS, but without evidence of aCL, anti2GPI antibodies or LA [16].…”

Study of Phosphatidylserine-dependent Anti-prothrombin Antibody in Cerebral Infarction

“…IgG aPS/PT is an autoantibody directed against phosphatidylserineprothrombin complex (7,8), and is thought to be significantly related to APS (2).…”

Abdominal Pain, Flank Pain, Blurry Vision, and Lower Extremity Weakness in a 16‐Year‐Old Female

“…In 2006, microvascular thrombosis, such as thrombotic microangiopathy (TMA), was incorporated in APS as an expanded disease spectrum (1,2). aPL are a group of antibodies directed against proteins containing anionic phospholipids, including ␤ 2 GPI, aca-cofactor, prothrombin, and phosphatidylserine/prothrombin complex (3)(4)(5). The presence of aPL can occur because of primary idiopathic or secondary to autoimmune rheumatic diseases, infections, medications, and malignancies.…”