Antiprotozoal activity of Boesenbergia rotunda (L.) Mansf and Ganoderma lucidum (Fr.) Kart extracts against Blastocystis hominis

Kaewjai, Chalermpon; Tonsomboon, Aunchalee; Pawiwongchai, Jaturawat; Prommano, Oranan

doi:10.14202/vetworld.2023.187-193

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Among them, Blastocystis spp. is the most widely distributed protozoa ( Kaewjai et al., 2023 ). Similar clinical symptoms and different treatment methods highlight the importance of establishing a rapid and accurate diagnostic method for Blastocystis spp.…”

Section: Discussionmentioning

confidence: 99%

Recombinase polymerase amplification - lateral flow dipstick for rapid and visual detection of Blastocystis spp.

Mei,

Su,

Xin

et al. 2024

Front. Cell. Infect. Microbiol.

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Blastocystis spp. is a ubiquitous protozoon in the intestinal tract of human and many animals. Microscopic examination is the main method of clinical diagnosis for Blastocystis spp., which is prone to false negative. A simple and rapid diagnosis of Blastocystis spp. infection is an important step to prevent and control blastocystosis. Here, a recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay was developed for rapid visual detection of Blastocystis spp. DNA amplification could be performed within 18 min at 37°C. The minimum DNA detection limit was 1 pg/μL, and there was no cross-reactivity with 12 other non-target pathogens, which was consistent with the sensitivity of conventional PCR (cPCR). Furthermore, 56 fecal samples from the Third Affiliated Hospital of Xinxiang Medical University were tested using RPA and cPCR methods respectively, and the results were completely consistent. The results show that RPA-LFD method has high accuracy and visual results, which provides a new choice for the differential diagnosis and rapid field detection of Blastocystis spp.

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Section: Discussionmentioning

confidence: 99%

Recombinase polymerase amplification - lateral flow dipstick for rapid and visual detection of Blastocystis spp.

Mei,

Su,

Xin

et al. 2024

Front. Cell. Infect. Microbiol.

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show abstract

“…Among them, Blastocystis sp. is one of the most common intestinal protozoans detected in humans (Kaewjai et al ., 2023 ). Therefore, it is very important to establish a novel method for rapid and accurate diagnosis of Blastocystis sp.…”

Section: Introductionmentioning

confidence: 99%

Development and application of recombinase polymerase amplification assay for rapid detection of Blastocystis sp.

Mei,

Su,

Zhang

et al. 2023

Parasitology

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Blastocystis sp. is a common parasite in the intestinal tract of humans and animals. The clinical diagnosis of Blastocystis sp. mainly depends on the microscopic observation of parasite, which can lead to false-negative results. An accurate and convenient diagnostic approach for Blastocystis sp. infection is crucial for effectively preventing and controlling blastocystosis. Herein, we developed a recombinase polymerase amplification (RPA) method for detecting Blastocystis sp. The results showed that the DNA amplification by RPA established in this study could be performed within 5 min at 37°C, with maximum band intensity observed at 30 min. The minimum detection limit of RPA was 100 fg μL−1, consistent with conventional polymerase chain reaction (cPCR). Furthermore, the RPA method exhibited no cross-reactivity with 7 other non-target pathogens in the intestinal tract. Next, the newly established RPA method was used to analyse 40 fecal samples collected clinically, and the detection results were consistent with cPCR. These results corroborate that the newly developed RPA method has good sensitivity and specificity and offers the advantage of short detection times, which can be harnessed for differential diagnosis and rapid detection of Blastocystis sp.

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Ganoderma lucidum triterpenoids investigating their role in medicinal applications and genomic protection

Raza,

Zhong,

et al. 2024

Journal of Pharmacy and Pharmacology

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Objectives Ganoderma lucidum (GL) is a white rot fungus widely used for its pharmacological properties and health benefits. GL consists of several biological components, including polysaccharides, sterols, and triterpenoids. Triterpenoids are often found in GL in the form of lanostane-type triterpenoids with quadrilateral carbon structures. Key findings The study revealed that triterpenoids have diverse biological properties and can be categorized based on their functional groups. Triterpenoids derived from GL have shown potential medicinal applications. They can disrupt the cell cycle by inhibiting β-catenin or protein kinase C activity, leading to anti-cancer, anti-inflammatory, and anti-diabetic effects. They can also reduce the production of inflammatory cytokines, thus mitigating inflammation. Additionally, triterpenoids have been found to enhance the immune system’s defenses against various health conditions. They possess antioxidant, antiparasitic, anti-hyperlipidemic, and antimicrobial activities, making them suitable for pharmaceutical applications. Furthermore, triterpenoids are believed to afford radioprotection to DNA, protecting it from radiation damage. Summary This review focuses on the types of triterpenoids isolated from GL, their synthesis pathways, and their chemical structures. Additionally, it highlights the pharmacological characteristics of triterpenoids derived from GL, emphasizing their significant role in various therapeutic applications and health benefits for both humans and animals.

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Antiprotozoal activity of Boesenbergia rotunda (L.) Mansf and Ganoderma lucidum (Fr.) Kart extracts against Blastocystis hominis

Cited by 3 publications

References 29 publications

Recombinase polymerase amplification - lateral flow dipstick for rapid and visual detection of Blastocystis spp.

Recombinase polymerase amplification - lateral flow dipstick for rapid and visual detection of Blastocystis spp.

Development and application of recombinase polymerase amplification assay for rapid detection of Blastocystis sp.

Ganoderma lucidum triterpenoids investigating their role in medicinal applications and genomic protection

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