Antiprotozoal activity of medicinal plants used by Iquitos-Nauta road communities in Loreto (Peru)

Vásquez-Ocmín, Pedro; Cojean, Sandrine; Rengifo, Elsa; Suyyagh-Albouz, Soulaf; Guerra, Carlos A. Amasifuén; Pomel, Sébastien; Cabanillas, Billy; Mejía, Kember; Loiseau, Philippe M.; Figadère, Bruno; Maciuk, Alexandre

doi:10.1016/j.jep.2017.08.039

Cited by 37 publications

(51 citation statements)

References 53 publications

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“…Traditional medicine in local indigenous communities uses piton properties for treatment of several pathological conditions including sinusitis, uterine bleeding, diarrhea, constipation, among others [27]. Recently, Vásquez-Ocmín and coworkers demonstrated that a G. neuberthii bark extract had antiparasitic activity in vitro [28]. e application, however, of G. neuberthii extract in the synthesis of AgNPs has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Biological Effect of Organically CoatedGrias neuberthiiandPersea americanaSilver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines

Salazar

López

Grijalva

et al. 2018

Journal of Nanotechnology

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The aim of this study was to assess the biological effect of organically coated Grias neuberthii (piton) fruit and Persea americana (avocado) leaves nanoparticles (NPs) on cervical cancer (HeLa) and breast adenocarcinoma (MCF-7) cells with an emphasis on gene expression (p53 transcription factor and glutathione-S-transferase GST) and cell viability. UV-Vis spectroscopy analysis showed that synthesized AgNPs remained partially stable under cell culture conditions. HeLa cells remained viable when exposed to piton and avocado AgNPs. A statistically significant, dose-dependent cytotoxic response to both AgNPs was found on the breast cancer (MCF-7) cell line at concentrations above 50 µM. While expression levels of transcription factor p53 showed downregulation in treated MCF-7 and HeLa cells, GST expression was not affected in both cell lines treated. Cell viability assays along with gene expression levels in treated MCF-7 cells support a cancer cell population undergoing cell cycle arrest. The selective toxicity of biosynthesized piton/avocado AgNPs on MCF-7 cells might be of value for novel therapeutics.

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Section: Introductionmentioning

confidence: 99%

Biological Effect of Organically CoatedGrias neuberthiiandPersea americanaSilver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines

Salazar

López

Grijalva

et al. 2018

Journal of Nanotechnology

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“…Incorporation of SYBR ® Green I (Applied Biosystems, Strasbourg, France) in parasite DNA was measured using the Mastercycler epRealplex (Eppendorf, France) according to the following program to increase the SYBR ® Green I incorporation: 90 °C (1 min), decrease in temperature from 90 °C to 10 °C (during 5 min), followed by fluorescence reading. Untreated infected and uninfected erythrocytes were used as controls and chloroquine diphosphate (Sigma, France) as reference drug [39,40]. IC 50 were calculated by IC-estimator online software (http://www.antimalarialicestimator.net).…”

Section: In Vitro Antiplasmodial Activitymentioning

confidence: 99%

Correlation study on methoxylation pattern of flavonoids and their heme-targeted antiplasmodial activity

Ortíz

Vásquez-Ocmín

Cojean

et al. 2020

Bioorganic Chemistry

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A library of 33 polymethoxylated flavones (PMF) was evaluated for heme-binding affinity by biomimetic MS assay and in vitro antiplasmodial activity on two strains of P. falciparum. Stability of heme adducts was discussed using the dissociation voltage at 50% (DV 50). No correlation was observed between the methoxylation pattern and the antiparasitic activity, either for the 3D7 chloroquine-sensitive or for the W2 chloroquine-resistant P. falciparum strains. However, in each PMF family an increased DV 50 was observed for the derivatives methoxylated in position 5. Measurement of intra-erythrocytic hemozoin formation of selected derivatives was performed and hemozoin concentration was inversely correlated with heme-binding affinity. Kaempferol showed no influence on hemozoin formation, reinforcing the hypothesis that this compound may exert in vitro antiplasmodial activity mostly through other pathways. Pentamethoxyquercetin has simultaneously demonstrated a significant biological activity and a strong interaction with heme, suggesting that inhibition of hemozoin formation is totally or partially responsible for its antiparasitic effect.

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“…It inhibited a chloroquine-susceptible strain of P. falciparum 3D7 at doses lower than 10 µg/mL (toxicity to human blood lymphocytes was proven at a dose of 48 µg/mL) in vitro [3]. Recently, Vásquez-Ocmín et al reported a study of 50 extracts from 46 medicinal plants used traditionally against protozoan diseases in Loreto (Peru) and an ethanolic extract from the bark of M. macrocarpa was one of the most active against P. falciparum 3D7 chloroquine-sensitive strain and against P. falciparum W2 chloroquine-resistant strain with IC 50 = 0.02 µg/mL for both strains [62]. Pristimerin ( 26 ), an active compound isolated from M. macrocarpa , is unfortunately toxic to human cells (HT-29) [3,63], but only at concentrations approximately 10 times higher than required to kill P. falciparum K1 and P. falciparum NF54 (IC 50 190.4 and 270.9 ng/mL, respectively) [63].…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Maytenus macrocarpa (Ruiz & Pav.) Briq.: Phytochemistry and Pharmacological Activity

et al. 2019

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Maytenus macrocarpa (Celastraceae) is a tree native to Amazonia. Its roots, leaves, bark, and combinations of these are used in traditional medicine mainly to treat rheumatism and, to a lesser extent, to heal wounds and to combat bronchitis and diarrhea. To date, mainly triterpenes and dihydro-β-agarofuran sesquiterpenes were isolated from M. macrocarpa. Extracts and selected pure compounds isolated from the leaves, roots, and stem bark showed antibacterial, antiviral, antiparasitic, anti-inflammatory, and cytotoxic activities in vitro. The aim of this review is to summarize the available ethnobotanical, phytochemical, and pharmacological information about this traditional Amazonian medicinal tree, as well as to attract the attention of phytochemists and pharmacognosists to this potentially interesting source of ethnopharmaceuticals.

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Antiprotozoal activity of medicinal plants used by Iquitos-Nauta road communities in Loreto (Peru)

Cited by 37 publications

References 53 publications

Biological Effect of Organically CoatedGrias neuberthiiandPersea americanaSilver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines

Biological Effect of Organically CoatedGrias neuberthiiandPersea americanaSilver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines

Correlation study on methoxylation pattern of flavonoids and their heme-targeted antiplasmodial activity

Maytenus macrocarpa (Ruiz & Pav.) Briq.: Phytochemistry and Pharmacological Activity

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