Antipyretic and anti-inflammatory properties of the ethanolic extract, dichloromethane fraction and costunolide from Magnolia ovata (Magnoliaceae)

Kassuya, Cândida Aparecida Leite; Cremoneze, Aline; Barros, Letícia Ferrari Lemos; Simas, Alex Sandro; Lapa, Fernanda da Rocha; Mello‐Silva, Renato de; Stefanello, Maria Élida Alves; Zampronio, Aleksander Roberto

doi:10.1016/j.jep.2009.06.003

Cited by 71 publications

(62 citation statements)

References 33 publications

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“…The oral administration of costunolide inhibited carrageenan (Cg)-induced paw edema ) mg/ kg) and was effective in abolishing lipopolysaccharide (LPS)-induced fever (0.15 mg/kg) (Kassuya et al, 2009).…”

Section: Anti-inflammatorymentioning

confidence: 99%

Sesquiterpenoids in subtribe Centaureinae (Cass.) Dumort (tribe Cardueae, Asteraceae): Distribution, 13C NMR spectral data and biological properties

et al. 2013

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Section: Anti-inflammatorymentioning

confidence: 99%

Sesquiterpenoids in subtribe Centaureinae (Cass.) Dumort (tribe Cardueae, Asteraceae): Distribution, 13C NMR spectral data and biological properties

et al. 2013

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“…Costunolide was first isolated from costus roots (Saussurea lappa) (24) and is now found in several plant species, including Magnolia spp. and lettuce (25,26). It is known that costunolide is not only the precursor of STLs but also displays several bioactivities.…”

mentioning

confidence: 99%

“…It is known that costunolide is not only the precursor of STLs but also displays several bioactivities. Reported pharmaceutical activities of costunolide include anti-inflammatory (25), anti-carcinogenic (27), anti-diabetic (28), anti-fungal (29), and anti-viral activities (30). Hence, costunolide can be a valuable chemical feedstock as the precursor of various bioactive STLs.…”

mentioning

confidence: 99%

Lettuce Costunolide Synthase (CYP71BL2) and Its Homolog (CYP71BL1) from Sunflower Catalyze Distinct Regio- and Stereoselective Hydroxylations in Sesquiterpene Lactone Metabolism

Ikezawa

Göpfert

Nguyen

et al. 2011

Journal of Biological Chemistry

110

153

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Sesquiterpene lactones (STLs) are terpenoid natural products possessing the ␥-lactone, well known for their diverse biological and medicinal activities. The occurrence of STLs is sporadic in nature, but most STLs have been isolated from plants in the Asteraceae family. Despite the implication of the ␥-lactone group in many reported bioactivities of STLs, the biosynthetic origins of the ␥-lactone ring remains elusive. Germacrene A acid (GAA) has been suggested as a central precursor of diverse STLs. The regioselective (C6 or C8) and stereoselective (␣ or ␤) hydroxylation on a carbon of GAA adjacent to its carboxylic acid at C12 is responsible for the ␥-lactone formation. Here, we report two cytochrome P450 monooxygenases (P450s) capable of catalyzing 6␣-and 8␤-hydroxylation of GAA from lettuce and sunflower, respectively. To identify these P450s, sunflower trichomes were isolated to generate a trichome-specific transcript library, from which 10 P450 clones were retrieved. Expression of these clones in a yeast strain metabolically engineered to synthesize substrate GAA identified a P450 catalyzing 8␤-hydroxylation of GAA, but the STL was not formed by spontaneous lactonization. Subsequently, we identified the closest homolog of the GAA 8␤-hydroxylase from lettuce and discovered 6␣-hydroxylation of GAA by the recombinant enzyme. The resulting 6␣-hydroxy-GAA spontaneously undergoes a lactonization to yield the simplest form of STL, costunolide. Furthermore, we demonstrate the milligram per liter scale de novo synthesis of costunolide using the lettuce P450 in an engineered yeast strain, an important advance that will enable exploitation of STLs. Evolution and homology models of these two P450s are discussed.

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“…Saussurea costus is considered to be one of the antioxidant-rich medicinal plants. Many authors have reported that the roots of this plant possess various biological activities, including cortisol lowering effect [38], anti-infammatory [39,40], anticancer [41,42], antiviral [23], antimicrobial [43,44], antifungal [15], antioxidant [45,46], antidiabetic [47], antiulcer [20] and hepatoprotective effects [38]. Liv-52 is widely used in treatment of liver diseases of varying origins.…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective Effect of Costus Roots Extract against Carbon Tetrachloride (CCl4)-Induced Liver Injury in Guinea Pigs

Al-Duais¹,

Al-Awthan²

2017

JLS

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SC (Saussurea costus) (Asteraceae) is a traditionally known and potent plant which is well considered for its medicinal uses in different indigenous Indian systems of medicine. This study was undertaken to investigate the hepatoprotective effect of SC against liver injury induced by CCl 4 intoxication. Animals were divided into five groups. Group I, served as normal control. Group II received 2 mL CCl 4 /kg b.w. diluted with olive oil, at 1:1 ratio on day 11. Groups III and IV were pre-treated orally respectively with 100 and 200 mg/kg b.w. aqueous roots extract of SC for 10 days followed by subcutaneous injection of CCl 4 (2 mL/kg b.w.), once on day 11. Group V were orally given Liv-52 (100 mg/kg b.w.) once daily for 10 days followed by subcutaneous injection of CCl 4 (2 mL/kg b.w.), once on day 11. Our results show that, the activity of serum hepatic enzymes (ALT, AST, and ALP) were significantly elevated in guinea pigs treated with CCl 4 , while both the SC root extract and Liv-52 reduced significantly these enzymes activity. Also, the levels of glucose, urea and cholesterol were decreased when compared with intoxicated control. Histopathological examination of intoxicated animals showed dilation, hemorrhage, vacuolization, inflammation and necrosis indicating liver damage, while the animals received SC or Liv-52 that showed less pathological effects or normal liver when compared to animals treated with CCl 4 alone. Biochemical and histological results confirm the hepatoprotective effect of aqueous extract of SC.

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Antipyretic and anti-inflammatory properties of the ethanolic extract, dichloromethane fraction and costunolide from Magnolia ovata (Magnoliaceae)

Abstract: Collectively, these results show, for the first time, that extracts obtained from Magnolia ovata possess antipyretic and anti-inflammatory properties, and costunolide appears to be the compound responsible for these effects.

Cited by 71 publications

References 33 publications

Sesquiterpenoids in subtribe Centaureinae (Cass.) Dumort (tribe Cardueae, Asteraceae): Distribution, 13C NMR spectral data and biological properties

Sesquiterpenoids in subtribe Centaureinae (Cass.) Dumort (tribe Cardueae, Asteraceae): Distribution, 13C NMR spectral data and biological properties

Lettuce Costunolide Synthase (CYP71BL2) and Its Homolog (CYP71BL1) from Sunflower Catalyze Distinct Regio- and Stereoselective Hydroxylations in Sesquiterpene Lactone Metabolism

Hepatoprotective Effect of Costus Roots Extract against Carbon Tetrachloride (CCl4)-Induced Liver Injury in Guinea Pigs

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