Antipyretic and anti-nociceptive effects of methanol extract of leaves of Fimbristylis miliacea in mice model

Roy, Roni; Daula, A F M Shahid Ud; Akter, Afroza; Sultana, Sharmin; Barek, Md. Abdul; Liya, Israt Jahan; Basher, Mohammad Anwarul

doi:10.1016/j.jep.2019.112080

Cited by 23 publications

(12 citation statements)

References 19 publications

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“…(Simpson and Inglis, 2001). Methanol extract of F. miliacea has been found to have potent antipyretic and antinociceptive effect (Roy et al, 2019) and moderate hypoglycemic effect (Mia et al, 2019) in mice model.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical analysis, antioxidant and antidiarrhoeal activities of methanol extract of Fimbristylis miliacea (L.) Vahl

Mukta¹,

Roy²,

Daula³

et al. 2020

J. Pharmacognosy Phytother.

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The present study determined phytochemical content, antioxidant and antidiarrhoeal effect of methanol extract of Fimbristylis miliacea. Phytochemical screening showed the presence of flavonoids, saponins, tannins, phenols, alkaloids and cardiac glycosides. Total phenolic, flavonoids, flavonols, tannins, βcarotene, lycopene, chlorophyll-α, and chlorophyll-β contents were found 154.13 mg GAE/g, 83.14 mg QE/g, 126.45 mg QE/g, 215.72 mg GAE/g, 1.51 µg/g, 0.66 µg/g, 7.10 mg/g and 4.25 mg/g, respectively. Antioxidant potential was compared against ascorbic acid. IC 50 and EC 50 values were determined as indices of antioxidant potential using five experiments; IC 50 in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging assay were 138.83 and 353.82 µg/ml respectively. EC 50 of thiobarbituric acid assay, FRAP assay and reducing power assay were observed 2159.5, 31.59 and 129.56 µg/ml respectively. Total antioxidant capacity was 155.35 mg/g AAE. Significant reduction (p≤0.001) in fecal movement was produced by plant extract and the effect was stronger than that of the standard drug loperamide (5 mg/kg.bw). Loperamide showed 58.16% inhibition whereas extract at 400 mg/kg.bw dose inhibited 80.85% diarrhea.

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Section: Introductionmentioning

confidence: 99%

Phytochemical analysis, antioxidant and antidiarrhoeal activities of methanol extract of Fimbristylis miliacea (L.) Vahl

Mukta¹,

Roy²,

Daula³

et al. 2020

J. Pharmacognosy Phytother.

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“…Acetic acid-induced writhing test was used to evaluate the analgesic potential of meloxicam and its Ca(II) complex at 10 mg/Kg. Acetic acid-induced writhing test is a model of peripheral pain that is useful for anti-nociceptive drug development [ 64 ]. The Ca(II) complex showed more significant response (* P < 0.05) and reduced the number of characteristic writhing in rats as compared to meloxicam (Fig.…”

Section: Resultsmentioning

confidence: 99%

Synthesis, Spectroscopic and Biological Investigation of a New Ca(II) Complex of Meloxicam as Potential COX-2 Inhibitor

et al. 2022

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Drug development on basis of coordination compounds provides versatile structural and functional properties as compared to other organic compounds. In the present study, a new Ca(II) complex of meloxicam was synthesized and characterized by elemental analysis, FT-IR, UV–Vis, 13 C NMR, SEM–EDX, powder XRD and thermal analysis (TGA). The Ca(II) complex was investigated for its in vitro, in vivo biological activities and in silico docking analysis against COX-1 and COX-2. The spectral analysis indicates that the meloxicam acts as a deprotonated bidentate ligand (coordinated to the metal atom through the amide oxygen and the nitrogen atom of the thiazolyl ring) in the complex. SEM–EDX and powder XRD analysis depicted crystalline morphology of Ca(II) complex with a crystalline size of 32.86 nm. The in vitro biological activities were evaluated by five different antioxidant methods and COX inhibition assay, while in vivo activities were evaluated by carrageenan-, histamine- and PGE 2 -induced paw edema methods and acetic acid-induced writhing test. The Ca(II) complex showed prominent antioxidant activities and was found to be more selective toward COX-2 (43.77) than COX-1 as compared to meloxicam. It exhibited lower toxicity (LD 50 1000 mg/Kg) and significantly inhibited carrageenan- and PGE 2 -induced inflammation at 10 mg/Kg ( P < 0.05), but no significant effect was observed on histamine-induced inflammation. Moreover, Ca(II) complex significantly reduced the number of writhes induced by acetic acid ( P < 0.05). The in silico molecular docking data revealed that Ca(II) complex obstructed COX-2 (dock score 6438) more effectively than COX-1 (dock score 5732) as compared to meloxicam alone.

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“…The two classical pain models of the acetic acid–induced writhing and hot plate were employed for initial assessment on the feasibility of bufalin for pain management. Both tests were generally applied for a preliminary screening of potential drugs that might be used for chronic pain management ( Falade et al, 2019 ; Roy et al, 2019 ). Preliminary results on the abdominal writhing and the analgesic activity of bufalin in the rats after acetic acid injection was illustrated in Figure 5A .…”

Section: Resultsmentioning

confidence: 99%

Effect of Bufalin-PLGA Microspheres in the Alleviation of Neuropathic Pain via the CCI Model

et al. 2022

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The treatment of neuropathic pain (NPP) is considered challenging, while the search for alternative medication is striving. NPP pathology is related with the expression of both the purinergic 2X7 (P2X7) receptor and the transient receptor potential vanilloid 1 receptor (TRPV1). Bufalin is a traditional Chinese medication derived from toad venom with pronounced antitumor, analgesic, and anti-inflammatory properties. However, poor solubility, rapid metabolism, and the knowledge gap on its pain alleviation mechanism have limited the clinical application of bufalin. Hence, the purpose of this study is to illustrate the NPP alleviation mechanism of bufalin via chronic constriction injury (CCI). To address the concern on fast metabolism, bufalin-PLGA microspheres (MS) were prepared via membrane emulsification to achieve prolonged pain-relieving effects. Western blot, real-time PCR, immunofluorescence, and molecular docking were employed to demonstrate the therapeutic action of bufalin on NPP. The results showed enhanced thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) after the administration of both bufalin and bufalin-PLGA MS in the CCI rats. Prolonged pain-relieving effects for up to 3 days with reduced dose frequency was achieved via bufalin-PLGA MS. In the CCI rats treated with bufalin-PLGA MS, the expression levels of protein and mRNA in TRPV1 and P2X7, both localized in the dorsal root ganglion (DRG), were reduced. Moreover, bufalin-PLGA MS effectively reduced the levels of IL-1β, IL-18, IL-6, and TNF-α in the CCI group. The results from molecular docking suggested a possible mechanism of NPP alleviation of bufalin through binding to P2X7 receptors directly. The administration of bufalin-PLGA MS prepared by membrane emulsification demonstrated promising applications for sustained effect on the alleviation of NPP.

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Antipyretic and anti-nociceptive effects of methanol extract of leaves of Fimbristylis miliacea in mice model

Cited by 23 publications

References 19 publications

Phytochemical analysis, antioxidant and antidiarrhoeal activities of methanol extract of Fimbristylis miliacea (L.) Vahl

Phytochemical analysis, antioxidant and antidiarrhoeal activities of methanol extract of Fimbristylis miliacea (L.) Vahl

Synthesis, Spectroscopic and Biological Investigation of a New Ca(II) Complex of Meloxicam as Potential COX-2 Inhibitor

Effect of Bufalin-PLGA Microspheres in the Alleviation of Neuropathic Pain via the CCI Model

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