Three new 22-membered macrolactone antibiotics, atacamycins A-C, were produced by Streptomyces sp. C38, a strain isolated from a hyper-arid soil collected from the Atacama Desert in the north of Chile. The metabolites were discovered in our HPLC-diode array screening and isolated from the mycelium by extraction and chromatographic purification steps. The structures were determined by mass spectrometry and NMR experiments. Atacamycins A, B and C exhibited moderate inhibitory activities against the enzyme phosphodiesterase (PDE-4B2), whereas atacamycin A showed a moderate antiproliferative activity against adeno carcinoma and breast carcinoma cells.
INTRODUCTIONThe phylum Actinobacteria and particularly the genus Streptomyces is an excellent and in-exhausted source for the discovery of novel secondary metabolites with diverse biological activities based on unique pharmacophores. 1,2 High-quality isolates are a pre-requisite to prevent the rediscovery of known compounds as well as new sources for strain isolation. 3 Our focus for strain isolation is based on poorly studied habitats within the extremobiosphere. 4 The Atacama Desert in northern Chile is such an extreme habitat and is acknowledged to be the driest place on earth due to the rainshadow in front of the Andes mountains. 5 It is the oldest continuously arid desert, which has experienced extreme hyper-aridity for at least 150 million years of climatic stability. 6,7 Drees and coworkers 8 has shown that the hyper-arid soils of the Atacama Desert are not sterile but harbor a rich source of culturable bacteria, the majority of which were members of the phylum Actinobacteria.In a recently published study, the isolation of novel members of the order Actinomycetales from Atacama Desert soils was reported. 9 In this study, samples were collected at El Tatio (4300 m, geyser field), the Salar de Atacama (2300 m, salt flat hyper-arid) and the Valle de la Luna (2450 m, extreme hyper-arid). In all, 16 selected actinomycete isolates were added to our HPLC-diode array screening program to detect the production of novel secondary metabolites. In all, 3 of them