Antirestriction

Zavilgelsky, G. B.

doi:10.1007/bf02759614

Cited by 12 publications

(14 citation statements)

References 60 publications

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“…They therefore place a considerable evolutionary pressure on mobile genetic elements to evolve anti-restriction countermeasures encoded by the foreign DNA (2,3,7). One such measure is the use of anti-restriction proteins to modify or inactivate the RM system.…”

Section: Introductionmentioning

confidence: 99%

The structure of the KlcA and ArdB proteins reveals a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitro

Serfiotis-Mitsa

Herbert

Roberts

et al. 2009

Nucleic Acids Research

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Plasmids, conjugative transposons and phage frequently encode anti-restriction proteins to enhance their chances of entering a new bacterial host that is highly likely to contain a Type I DNA restriction and modification (RM) system. The RM system usually destroys the invading DNA. Some of the anti-restriction proteins are DNA mimics and bind to the RM enzyme to prevent it binding to DNA. In this article, we characterize ArdB anti-restriction proteins and their close homologues, the KlcA proteins from a range of mobile genetic elements; including an ArdB encoded on a pathogenicity island from uropathogenic Escherichia coli and a KlcA from an IncP-1b plasmid, pBP136 isolated from Bordetella pertussis. We show that all the ArdB and KlcA act as anti-restriction proteins and inhibit the four main families of Type I RM systems in vivo, but fail to block the restriction endonuclease activity of the archetypal Type I RM enzyme, EcoKI, in vitro indicating that the action of ArdB is indirect and very different from that of the DNA mimics. We also present the structure determined by NMR spectroscopy of the pBP136 KlcA protein. The structure shows a novel protein fold and it is clearly not a DNA structural mimic.

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Section: Introductionmentioning

confidence: 99%

The structure of the KlcA and ArdB proteins reveals a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitro

Serfiotis-Mitsa

Herbert

Roberts

et al. 2009

Nucleic Acids Research

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“…Evolution of all transmissible plasmids, conjugative transposones and some bacteriophages gave rise to systems enabling them to overcome restriction barriers. This phenomenon has been termed antirestriction (Zavilgelsky, 2000;Tock & Dryden, 2005). An investigation of antirestriction mechanisms employed by transmissible plasmids showed that the process involves a specialized antirestriction protein encoded by the ardA gene (alleviation of restriction of DNA).…”

Section: Conjugative Plasmids and Transposons Bacteriophages And Anmentioning

confidence: 99%

“…It has been supposed that antirestriction proteins of the ArdA family, as well as Ocr are modulator proteins with a structure similar to that of the B-form DNA, and the characteristic surface distribution of negatively charged D and E residues (aspartic and glutamic acids) imitates the distribution of negatively charged phosphate groups along the DNA double helix (Zavilgelsky, 2000). That is, antirestriction proteins imitate the DNA structure, which is currently termed "protein mimicry of DNA".…”

Section: Dna Mimicry By Antirestriction Proteinsmentioning

confidence: 99%

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DNA Mimicry by Antirestriction and Pentapeptide Repeat (PPR) Proteins

Zavilgelsky¹,

Kotova²

2012

Innovations in Biotechnology

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“…The ardA (alleviation of restriction of DNA) genes are responsible for the synthesis of small (160-170 amino acid residues), extremely acidic (total charge from -25 to -30) ArdA antirestriction proteins [1][2][3][4]. ardA genes occur in the leader region of trans missible (conjugative) plasmids and are among the first to enter the recipient cell during conjugative DNA transfer [3,4].…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of antirestriction activity of the ArdA and ArdB proteins encoded by genes of the R64 transmissible plasmid (IncI1)

2012

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Antirestriction proteins ArdA and ArdB are specific inhibitors of type I restriction-modification enzymes. The ardA and yfeB (ardB) genes were cloned from the transmissible plasmid R64 in the pUC18 and pZE21 vectors. The R64 ArdA and ArdB proteins were shown to inhibit only restriction activity of the type I restriction-modification enzyme (EcoKI) in Escherichia coli K12 cells. In contrast to ArdA, ArdB inhibited EcoKI restriction activity only at a high intracellular concentration. Antirestriction activity of ArdB did not depend on the ClpXP protease. The yfeB (ardB) gene of the R64 plasmid is transcribed from a weak promoter located upstream of yfeA.

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Antirestriction

Cited by 12 publications

References 60 publications

The structure of the KlcA and ArdB proteins reveals a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitro

The structure of the KlcA and ArdB proteins reveals a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitro

DNA Mimicry by Antirestriction and Pentapeptide Repeat (PPR) Proteins

Comparative analysis of antirestriction activity of the ArdA and ArdB proteins encoded by genes of the R64 transmissible plasmid (IncI1)

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