Antischistosomiasis Liver Fibrosis Effects of Chlorogenic Acid through IL-13/miR-21/Smad7 Signaling Interactions
            <i>In Vivo</i>
            and
            <i>In Vitro</i>

Wang, Yao; Yang, Fan; Xue, Jun; Zhou, Xuan; Luo, Lei; Ma, Qian; Chen, Yunfei; Zhang, Juan; Zhang, Shuling; Zhao, Lei

doi:10.1128/aac.01347-16

Cited by 45 publications

(39 citation statements)

References 52 publications

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“…The results of a study, in which hepatic stellate LX2 cells and CCl 4 -induced liver fibrosis model rats are used, indicated that CGA inhibited the mRNA expression of miR-21, Smad7, connective tissue growth factor, α-smooth muscle actin, tissue inhibitor of metalloproteinase 1, MMP-9, and transforming growth factor-β1 (TGF-β1), suggesting that CGA relieves liver fibrosis through the miR-21-regulated TGF-β1/Smad7 signaling pathway [ 136 ]. Similar results were reported by Wang et al [ 137 ] who showed that CGA could inhibit schistosomiasis-induced liver fibrosis through IL-13/miR-21/Smad7 signaling interactions in LX2 cells and schistosome-infected mice. Since liver fibrosis is a key factor for the risk of HCC [ 138 ], CGA might be useful for its prevention.…”

Section: Anti-cancer Effects Of Coffeesupporting

confidence: 90%

Anti-Cancer Effects of Green Tea Epigallocatchin-3-Gallate and Coffee Chlorogenic Acid

et al. 2020

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Tea and coffee are consumed worldwide and epidemiological and clinical studies have shown their health beneficial effects, including anti-cancer effects. Epigallocatechin gallate (EGCG) and chlorogenic acid (CGA) are the major components of green tea polyphenols and coffee polyphenols, respectively, and believed to be responsible for most of these effects. Although a large number of cell-based and animal experiments have provided convincing evidence to support the anti-cancer effects of green tea, coffee, EGCG, and CGA, human studies are still controversial and some studies have suggested even an increased risk for certain types of cancers such as esophageal and gynecological cancers with green tea consumption and bladder and lung cancers with coffee consumption. The reason for these inconsistent results may have been arisen from various confounding factors. Cell-based and animal studies have proposed several mechanisms whereby EGCG and CGA exert their anti-cancer effects. These components appear to share the common mechanisms, among which one related to reactive oxygen species is perhaps the most attractive. Meanwhile, EGCG and CGA have also different target molecules which might explain the site-specific differences of anti-cancer effects found in human studies. Further studies will be necessary to clarify what is the mechanism to cause such differences between green tea and coffee.

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Section: Anti-cancer Effects Of Coffeesupporting

confidence: 90%

Anti-Cancer Effects of Green Tea Epigallocatchin-3-Gallate and Coffee Chlorogenic Acid

et al. 2020

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“…3 ), but not by amikacin. Independent studies of M. abscessus cultures growing in a different medium carried out in Ghosh lab at the Wadsworth Center have shown that amikacin can induce whiB7 , but the response was much weaker than that of other antibiotics tested; higher concentrations were required, the fold induction was lower, and the response was delayed ( 41 ). The M. tuberculosis genome does not contain eis2 ; therefore, WhiB7 is linked to amikacin resistance in M. abscessus but not in M. tuberculosis .…”

Section: Discussionmentioning

confidence: 99%

Antagonism between Front-Line Antibiotics Clarithromycin and Amikacin in the Treatment of Mycobacterium abscessus Infections Is Mediated by the whiB7 Gene

Pryjma

Burian

Kuchinski

et al. 2017

Antimicrob Agents Chemother

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Combinations of antibiotics, each individually effective against Mycobacterium abscessus, are routinely coadministered based on the concept that this minimizes the spread of antibiotic resistance. However, our in vitro data contradict this assumption and instead document antagonistic interactions between two antibiotics (clarithromycin and amikacin) used to treat M. abscessus infections. Clinically relevant concentrations of clarithromycin induced increased resistance to both amikacin and itself. The induction of resistance was dependent on whiB7, a transcriptional activator of intrinsic antibiotic resistance that is induced by exposure to many different antibiotics. In M. abscessus, the deletion of whiB7 (MAB_3508c) resulted in increased sensitivity to a broad range of antibiotics. WhiB7 was required for transcriptional activation of genes that confer resistance to three commonly used anti-M. abscessus drugs: clarithromycin, amikacin, and tigecycline. The whiB7-dependent gene that conferred macrolide resistance was identified as erm(41) (MAB_2297), which encodes a ribosomal methyltransferase. The whiB7-dependent gene contributing to amikacin resistance was eis2 (MAB_4532c), which encodes a Gcn5-related N-acetyltransferase (GNAT). Transcription of whiB7 and the resistance genes in its regulon was inducible by subinhibitory concentrations of clarithromycin but not by amikacin. Thus, exposure to clarithromycin, or likely any whiB7-inducing antibiotic, may antagonize the activities of amikacin and other drugs. This has important implications for the management of M. abscessus infections, both in cystic fibrosis (CF) and non-CF patients.

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“…The abundance of PPARγ, KLF4, SOCS1, STAT6 and p-STAT6 was detected by Western blot. The operation and analysis were performed as described in a previously published paper (Wang et al, 2017 ). The protein was separated on SDS-PAGE gels and then transferred to nitrocellulose filter membrane, which was blocked overnight with 5% non-fat milk in TBST.…”

Section: Methodsmentioning

confidence: 99%

Corilagin Counteracts IL-13Rα1 Signaling Pathway in Macrophages to Mitigate Schistosome Egg-Induced Hepatic Fibrosis

Chen

Dang

et al. 2017

Front. Cell. Infect. Microbiol.

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The IL-13Rα1 signaling pathway and M2 macrophages play crucial roles in schistosome egg-induced hepatic fibrosis via the expression of pro-fibrotic molecules. This study aims to investigate the inhibitory effect and mechanism of action of corilagin on schistosome egg-induced hepatic fibrosis via the IL-13Rα1 signaling pathway in M2 macrophages in vitro and in vivo. The mRNA and protein expression of IL-13Rα1, PPARγ, KLF4, SOCS1, STAT6, p-STAT6, and TGF-β was measured in vitro with corilagin treatment after IL-13 stimulation and in vivo corilagin treatment after effectively killing the adult schistosomes in schistosome-infected mice. Histological analysis of liver tissue was assessed for the degree of hepatic fibrosis. The results revealed that corilagin significantly reduced the expression of PPARγ, KLF4, SOCS1, p-STAT6, and TGF-β compared with model group and praziquantel administration (p < 0.01 or p < 0.05) in vivo and in vitro, which indicated a strong inhibitory effect of corilagin on IL-13Rα1 signaling pathway. As well, the inhibitory effect of corilagin showed a significant dose-dependence (p < 0.05). The area of fibrosis and distribution of M2 macrophages in mouse liver tissue were reduced significantly and dose-dependently with corilagin treatment compared to model group or praziquantel administration (p < 0.01 or p < 0.05), indicating that corilagin suppressed IL-13Rα1 signaling pathway and M2 macrophage polarization effectively in vivo. Furthermore, the anti-fibrogenic effect persisted even when IL-13Rα1 was up- or down-regulated in vitro. In conclusion, corilagin can suppress schistosome egg-induced hepatic fibrosis via inhibition of M2 macrophage polarization in the IL-13Rα1 signaling pathway.

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Antischistosomiasis Liver Fibrosis Effects of Chlorogenic Acid through IL-13/miR-21/Smad7 Signaling Interactions In Vivo and In Vitro

Abstract: This study investigated the antischistosomiasis liver fibrosis effects of chlorogenic acid (CGA) on interleukin 13 (IL-13)/microRNA-21 (miR-21)/Smad7 signaling interactions in the hepatic stellate LX2 cell line and schistosome-infected mice.

Cited by 45 publications

References 52 publications

Anti-Cancer Effects of Green Tea Epigallocatchin-3-Gallate and Coffee Chlorogenic Acid

Anti-Cancer Effects of Green Tea Epigallocatchin-3-Gallate and Coffee Chlorogenic Acid

Antagonism between Front-Line Antibiotics Clarithromycin and Amikacin in the Treatment of Mycobacterium abscessus Infections Is Mediated by the whiB7 Gene

Corilagin Counteracts IL-13Rα1 Signaling Pathway in Macrophages to Mitigate Schistosome Egg-Induced Hepatic Fibrosis

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