Antiseizure effects of Lilii Bulbus on pentylenetetrazol kindling-induced seizures in mice: Involvement of Reelin, Netrin-1, and semaphorin

Park, Hee Ra; Cai, Mudan

doi:10.1016/j.biopha.2024.116385

Biomedicine & Pharmacotherapy

2024

DOI: 10.1016/j.biopha.2024.116385

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Antiseizure effects of Lilii Bulbus on pentylenetetrazol kindling-induced seizures in mice: Involvement of Reelin, Netrin-1, and semaphorin

Hee Ra Park,

Mudan Cai

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2024

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Cited by 2 publications

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Kindling Models of Epileptogenesis for Developing Disease‐Modifying Drugs for Epilepsy

Reddy,

Vadassery,

Ramakrishnan

et al. 2024

Current Protocols

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Kindling models are widely used animal models to study the pathobiology of epilepsy and epileptogenesis. These models exhibit distinctive features whereby sub‐threshold stimuli instigate the initial induction of brief focal seizures. Over time, the severity and duration of these seizures progressively increase, leading to a fully epileptic state, which is marked by consistent development of generalized tonic‐clonic seizures. Kindling involves focal stimulation via implanted depth electrodes or repeated administration of chemoconvulsants such as pentylenetetrazol. Comparative analysis of preclinical and clinical findings has confirmed a high predictive validity of fully kindled animals for testing novel antiseizure medications. Thus, kindling models remain an essential component of anticonvulsant drug development programs. This article provides a comprehensive guide to working protocols, testing of therapeutic drugs, outcome parameters, troubleshooting, and data analysis for various electrical and chemical kindling epileptogenesis models for new therapeutic development and optimization. The use of pharmacological agents or genetically modified mice in kindling experiments is valuable, offering insights into the impact of a specific target on various aspects of seizures, including thresholds, initiation, spread, termination, and the generation of a hyperexcitable network. These kindling epileptogenesis paradigms are helpful in identifying mechanisms and disease‐modifying interventions for epilepsy. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Hippocampal kindlingBasic Protocol 2: Amygdala kindlingBasic Protocol 3: Rapid hippocampal kindlingBasic Protocol 4: Chemical kindling

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Kindling Models of Epileptogenesis for Developing Disease‐Modifying Drugs for Epilepsy

Reddy,

Vadassery,

Ramakrishnan

et al. 2024

Current Protocols

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The HPLC–PDA Method for Simultaneous Determination of Regalosides from Bulbs of Lilium lancifolium Thunb. and Their Antioxidant Effects

Seo,

Kim,

Song

2024

Plants

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Lilium lancifolium Thunb. is a herbal medicine that is widely used to treat inflammation and lung diseases. In this study, a simultaneous quantitative method was developed for the quality control of BLL using high-performance liquid chromatography coupled with a photodiode array detector (HPLC–PDA), and their antioxidant effects were evaluated. Eight regalosides (i.e., regaloside A, B, C, E, F, H, I, and K) were selected as marker substances and separated on a Gemini C18 reversed-phase analytical column by gradient elution with distilled water–acetonitrile mobile phase containing 0.1% (v/v) formic acid. The method was validated with respect to linearity, sensitivities (limit of detection (LOD) and limit of quantitation (LOQ)), accuracy, and precision. The antioxidant effects of the extract and each component were evaluated using the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay and 2-2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay. The coefficients of determination values used as indicators of linearity for all components were ≥0.9999. LOD and LOQ concentrations were 0.10–0.66 μg/mL and 0.29–2.01 μg/mL, respectively. The recovery was 95.39–103.925% (relative standard deviation; RSD ≤ 2.55%), and precision RSD was <2.78%. The HPLC–PDA method was applied to real samples, and all components were detected at 1.12–29.76 mg/freeze-dried g. The evaluation of antioxidant effects showed that regalosides C, E, and K exhibited significant antioxidant effects. Our knowledge will be appropriately utilized in raw material management and conducting clinical and non-clinical studies on L. lancifolium or herbal medicine prescriptions containing L. lancifolium.

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Antiseizure effects of Lilii Bulbus on pentylenetetrazol kindling-induced seizures in mice: Involvement of Reelin, Netrin-1, and semaphorin

Cited by 2 publications

References 52 publications

Kindling Models of Epileptogenesis for Developing Disease‐Modifying Drugs for Epilepsy

Kindling Models of Epileptogenesis for Developing Disease‐Modifying Drugs for Epilepsy

The HPLC–PDA Method for Simultaneous Determination of Regalosides from Bulbs of Lilium lancifolium Thunb. and Their Antioxidant Effects

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