Antisense apple ACC-oxidase RNA reduces ethylene production in transgenic tomato fruit

Bolitho, Karen M.; Lay-Yee, Michael; Knighton, Michelle L.; Ross, G. J.

doi:10.1016/s0168-9452(96)04532-3

Cited by 31 publications

(7 citation statements)

References 29 publications

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“…pathway during fmit ripening originally came from stud-Biochemical insights into the onset of autostimulatory ies describing ACC accumulation and increases in ACO ethylene production in climacteric fmit have been proand ACS activities in a variety of climacteric fmits vided by the analysis of transgenic and mutant plants Hoffman 1984, Abeles et al 1992). Since with altered levels of ethylene production or sensitivity, then, transgenic plants with reduced ethylene production Autocatalytic ethylene production requires upregulation (Hamilton et al 1990, Klee et al 1991 by ethylene of the enzymes involved in the rate-limiting 1991, Good et al 1994, Ayub et al 1996, Howie et al reactions in ethylene biosynthesis, namely ACS and 1996, Bolitho et al 1997 and potent new ethylene an-ACO. In tomato ) and melon (Guis et tagonists have been developed (Serek andSisler 1997).…”

Section: Introductionmentioning

confidence: 99%

Ethylene and fruit ripening

Lelièvre

Latché

Jones

et al. 1997

Physiologia Plantarum

606

284

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The latest advances in our understanding of the relationship between ethylene and fruit ripening are reviewed. Considerable progress has been made in the characterisation of genes encoding the key ethylene biosynthetic enzymes, ACC synthase (ACS) and ACC oxidase (ACO) and in the isolation of genes involved in the ethylene signal transduction pathway, particularly those encoding ethylene receptors (ETR). These have allowed the generation of transgenic fruit with reduced ethylene production and the identification of the Nr tomato ripening mutant as an ethylene receptor mutant. Through these tools, a clearer picture of the role of ethylene in fruit ripening is now emerging. In climacteric fruit, the transition to autocatalytic ethylene production appears to result from a series of events where developmentally regulated ACO and ACS gene expression initiates a rise in ethylene production, setting in motion the activation of autocatalytic ethylene production. Differential expression of ACS and ACO gene family members is probably involved in such a transition. Finally, we discuss evidence suggesting that the NR ethylene perception and transduction pathway is specific to a defined set of genes expressed in ripening climacteric fruit and that a distinct ETR pathway regulates other ethylene‐regulated genes in both immature and ripening climacteric fruit as well as in non‐climacteric fruit. The emerging picture is one where both ethylene‐dependent and ‐independent pathways coexist in both climacteric and non‐climacteric fruits. Further work is needed in order to dissect the molecular events involved in individual ripening processes and to understand the regulation of the expression of both ethylene‐dependent and ‐independent genes.

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Section: Introductionmentioning

confidence: 99%

Ethylene and fruit ripening

Lelièvre

Latché

Jones

et al. 1997

Physiologia Plantarum

606

284

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show abstract

“…Similarly, antagonists of ethyltion pathways (Ecker 1995), and a clearer picture of the ene action enhance ethylene synthesis in immature torole of ethylene in fmit ripening is now emerging. Since with altered levels of ethylene production or sensitivity, then, transgenic plants with reduced ethylene production Autocatalytic ethylene production requires upregulation (Hamilton et al 1990, Klee et al 1991 ethylene of the enzymes involved in the rate-limiting 1991, Good et al 1994, Ayub et al 1996, Howie et al reactions in ethylene biosynthesis, namely ACS and 1996, Bolitho et al 1997 and potent new ethylene an-ACO. Autoinhibition of eth-It is well documented (Yang and Hoffman 1984) that ylene production is also seen in non-climacteric fmit the pathway of ethylene biosynthesis proceeds from me-such as in wounded flavedo tissues of citrus (Riov and thionine, through S-adenosylmethionine (SAM) and 1- .…”

Section: Introductionmentioning

confidence: 99%

Ethylene and fruit ripening

Lelièvre¹,

Latché²,

Bouzayen³

et al. 1997

Physiol Plant

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The latest advances in our understanding of the relationship between ethylene and fmit ripening are reviewed. Considerable progress has been made in the characterisation of genes encoding the key ethylene biosynthetic enzymes, ACC synthase (ACS) and ACC oxidase (ACO) and in the isolation of genes involved in the ethylene signal transduction pathway, particularly those encoding ethylene receptors (ETR). These have allowed the generation of transgenic fmit with reduced ethylene production and the identification of the Nr tomato ripening mutant as an ethylene receptor mutant. Through these tools, a clearer picture of the role of ethylene in fmit ripening is now emerging. In climacteric fmit, the transition to autocatalytic ethylene production appears to result from a series of events where developmentally regulated ACO and ACS gene expression initiates a rise in ethylene production, setting in motion the activation of autocatalytic ethylene production. Differential expression of ACS and ACO gene family members is probably involved in such a transition. Finally, we discuss evidence suggesting that the NR ethylene perception and transduction pathway is specific to a defined set of genes expressed in ripening climacteric fmit and that a distinct ETR pathway regulates other ethylene-regulated genes in both immature and ripening climacteric fruit as well as in non-climacteric fmit. The emerging picture is one where both ethylene-dependent and -independent pathways coexist in both climacteric and non-climacteric fmits. Further work is needed in order to dissect the molecular events involved in individual ripening processes and to understand the regulation of the expression of both ethylene-dependent and -independent genes.

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“…Relatively few studies have been undertaken to characterize the molecular basis of fruit development. Most molecular studies on fruit have concentrated on fruit ripening through the characterization of ethylene biosynthesis genes (Bolitho et al, 1997;Grierson and Fray, 1994;Hamilton et al, 1990) and cell wall softening genes such as polygalacturonase (Kramer andRedenbaugh, 1994, Watson et al, 1994). A few cDNA clones have been isolated from young fruit cDNA libraries using differential screening (Dong et al, 1997;Ledger and Gardner, 1994;Santino et al, 1997) or differential display techniques (Tieman and Handa, 1996).…”

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confidence: 99%

Seven MADS-box Genes in Apple are Expressed in Different Parts of the Fruit

Yao¹,

Dong²,

Kvarnheden³

et al. 1999

jashs

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To study the role of MADS-box genes in developing apples (Malus ×domestica Borkh.), clones corresponding to seven different genes, MdMADS5 to MdMADS11, were isolated from a 2-day-old apple cDNA library. Through DNA sequence comparison, six genes were classified into the APETALA1 (AP1) group and one gene, MdMADS10, into the AGAMOUS (AG) group. Six of the genes, MdMADS5 to MdMADS10, were found to be preferentially expressed in fruit following pollination. These genes also showed differential expression patterns in core, cortex and skin of young fruit. For instance, MdMADS5, which is highly homologous to AP1, showed preferential expression in the cortex and skin tissues while MdMADS10, which is highly homologous to AGL11, showed exclusive expression in the core tissues. The gene MdMADS11 showed a similar expression level and pattern in flowers, fruit at several early developmental stages, and for different fruit tissues. The range of expression patterns suggests that the genes play different roles in apple development.

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Antisense apple ACC-oxidase RNA reduces ethylene production in transgenic tomato fruit

Cited by 31 publications

References 29 publications

Ethylene and fruit ripening

Ethylene and fruit ripening

Ethylene and fruit ripening

Seven MADS-box Genes in Apple are Expressed in Different Parts of the Fruit

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