Antisense down-regulation of the strawberry β-galactosidase gene<i>FaβGal4</i>increases cell wall galactose levels and reduces fruit softening

Paniagua, Candelas; Blanco‐Portales, Rosario; Barceló, Marta; García-Gago, Juan Antonio; Waldron, Keith W.; Quesada, Miguel A.; Muñoz‐Blanco, Juan; Mercado, José A.

doi:10.1093/jxb/erv462

Cited by 124 publications

(80 citation statements)

References 54 publications

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“…The crude cell wall content of control and COS-treated fruits were 1.8% and 2.2%, respectively. The strawberry fruit softening has been characterized by the solubilization and depolymerization of pectins from cell walls [22]. According to previous report, the ionic pectin, its covalent bonding, hemicellulose, and cellulose content were closely related to the texture [21].…”

Section: Resultsmentioning

confidence: 99%

Pre-Harvest Treatment of Chitosan Oligosaccharides Improved Strawberry Fruit Quality

Bose

Wang

et al. 2018

IJMS

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Chitosan oligosaccharide (COS), derived through hydrolysis of chitosan, has been proved to be an effective plant immunity elicitor, eco-friendly, and easily soluble in water, and influenced several secondary metabolites content to improve fruit qualities. COS are widely used in agriculture to improve the defense response in plants. The purpose of this study was to investigate the pre-harvest treatment effect of COS on the quality of strawberry (Fragaria × ananassa cv. qingxiang). COS was dissolved in distilled water at a concentration of 50 mg·L−1 and sprayed at four different growth stages of strawberry plants, namely seedling stage, before flowering, fruit coloring (the stage of fruit from white to red) and full bloom. Uniform size, shape, color, without any visible damage, and disease-free fruits were harvested for determining the quality. The results showed that the fruit firmness, viscosity, lignin, sugars, protein, total soluble solid, and titratable acidity content increased in COS-treated fruits compared to control. In addition, COS pre-harvest treatment had a positive effect on anthocyanin, total phenol, flavonoid, vitamin C content and DPPH(2,2-diphenyl-1-picrylhydrazyl) scavenging activity of strawberry. Moreover, COS also increased the cell wall composition and regulated gene expression of some important enzymes involved in ethylene compound biosynthesis and cell wall degradation. The finding of this study suggests that pre-harvest application of COS is very useful for improving quality and antioxidant capacity of strawberry.

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Section: Resultsmentioning

confidence: 99%

Pre-Harvest Treatment of Chitosan Oligosaccharides Improved Strawberry Fruit Quality

Bose

Wang

et al. 2018

IJMS

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“…RT-qPCR amplification was performed under the following conditions: 40 cycles at 95°C for 30 s and 58°C for 30 s. After amplification, the samples were maintained at 50°C for 1 min, and the temperature was gradually increased by 0.5°C every 10 s to perform the melting curve analysis. The interspacer 26S-18S strawberry RNA gene (housekeeping gene; Fv18S ) was used as an internal control to normalize the expression data (Raab et al, 2006; Hu et al, 2015; Medina-Puche et al, 2015; Paniagua et al, 2016; Wei et al, 2016). Three biological replicates with three technical replicates were assayed for each sample.…”

Section: Methodsmentioning

confidence: 99%

Identification and Transcript Analysis of the TCP Transcription Factors in the Diploid Woodland Strawberry Fragaria vesca

et al. 2016

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Plant-specific TEOSINTE BRANCHED 1, CYCLOIDEA, and PROLIFERATING CELL FACTORS (TCP) transcription factors play versatile functions in multiple processes of plant growth and development. However, no systematic study has been performed in strawberry. In this study, 19 FvTCP genes were identified in the diploid woodland strawberry (Fragaria vesca) accession Heilongjiang-3. Phylogenetic analysis suggested that the FvTCP genes were classified into two main classes, with the second class further divided into two subclasses, which was supported by the exon-intron organizations and the conserved motif structures. Promoter analysis revealed various cis-acting elements related to growth and development, hormone and/or stress responses. We analyzed FvTCP gene transcript accumulation patterns in different tissues and fruit developmental stages. Among them, 12 FvTCP genes exhibited distinct tissue-specific transcript accumulation patterns. Eleven FvTCP genes were down-regulated in different fruit developmental stages, while five FvTCP genes were up-regulated. Transcripts of FvTCP genes also varied with different subcultural propagation periods and were induced by hormone treatments and biotic and abiotic stresses. Subcellular localization analysis showed that six FvTCP-GFP fusion proteins showed distinct localizations in Arabidopsis mesophyll protoplasts. Notably, transient over-expression of FvTCP9 in strawberry fruits dramatically affected the expression of a series of genes implicated in fruit development and ripening. Taken together, the present study may provide the basis for functional studies to reveal the role of this gene family in strawberry growth and development.

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“…PMEs catalyzed the de‐esterification of GalA from HG backbone, allowing calcium bridges formation and also providing the substrate of pectinases such as PGs and PLs . Besides the structure preservation of HGs, it has been shown that the side‐chain conformation of the RG‐I are tightly related to fruit firmness, being the study of β‐Gal and α‐Ara activities a matter of interest …”

Section: Discussionmentioning

confidence: 99%

Calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (Fragaria × ananassa, Duch)

et al. 2019

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BACKGROUND Fruit dips in calcium ions solutions have been shown as an effective treatment to extend strawberries (Fragaria × ananassa, Duch) quality during storage. In the present work, strawberry fruit were treated with 10 g L−1 calcium chloride solution and treatment effects on cell wall enzymes activities and the expression of encoding genes, as well as enzymes involved in fruit defense responses were investigated. RESULTS Calcium treatment enhanced pectin methylesterase activity while inhibited those corresponding to pectin hydrolases as polygalacturonase and β‐galactosidase. The expression of key genes for strawberry pectin metabolism was up‐regulated (for FaPME1) and down‐regulated (for FaPG1, FaPLB, FaPLC, FaβGal1 and FaAra1) by calcium dips. In agreement, a higher firmness level and ionically‐bound pectins (IBPs) amount were detected in calcium‐treated fruit compared with controls. The in vitro and in vivo growth rate of fungal pathogen Botrytis cinerea was limited by calcium treatment. Moreover, the activities of polyphenol oxidases, chitinases, peroxidases and β‐1,3‐glucanases were enhanced by calcium ion dips. CONCLUSION News insights concerning the biochemical and molecular basis of cell wall preservation and resistance to fungal pathogens on calcium‐treated strawberries are provided. © 2019 Society of Chemical Industry

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Antisense down-regulation of the strawberry β-galactosidase geneFaβGal4increases cell wall galactose levels and reduces fruit softening

Abstract: HighlightWe identified a strawberry β-galactosidase gene involved in fruit ripening. Its silencing increases cell wall galactose levels and fruit firmness, suggesting galactose metabolism has a key role in strawberry softening.

Cited by 124 publications

References 54 publications

Pre-Harvest Treatment of Chitosan Oligosaccharides Improved Strawberry Fruit Quality

Pre-Harvest Treatment of Chitosan Oligosaccharides Improved Strawberry Fruit Quality

Identification and Transcript Analysis of the TCP Transcription Factors in the Diploid Woodland Strawberry Fragaria vesca

Calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (Fragaria × ananassa, Duch)

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