Antisense expression of Gossypium hirsutum UDP-glucuronate decarboxylase in Arabidopsis leads to changes in cell wall components

Zhang, D M; Pan, Yuxin; Zhang, Y; Li, Z K; Wu, Liqiang; Liu, H W; Zhang, G Y; Wang, X F; Y, Z

doi:10.4238/gmr.15017409

Cited by 2 publications

(3 citation statements)

References 29 publications

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“…Our results found that almost all DEGs of three carbohydrate metabolism pathways ("starch and sucrose metabolism", "galactose metabolism" and "pentose and glucuronate interconversions") were up-regulated in "T3 vs R" and "T4 vs H", and these DEGs were related to xylose, glucose, and galactose metabolism. For example, The UXS genes which were up-regulated here were positive regulators of xylose, glucose, and galactose in tobacco or Arabidopsis by the RNAi transgenic experiments [28,29]. These genes' up-regulation indicated the activation of the production of xylose, galactose and glucose related metabolites.…”

Section: Discussionmentioning

confidence: 82%

Continuous inflorescence removal changes parameters, lignin and saponin a content and alters the transcriptome of root of Bupleurum chinense DC

Wang

Zhang

Gao

et al. 2020

Preprint

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BackgroundBupleurum chinense DC. is an important traditional medicinal plant, and its root as the only medicinal part named Radix Bupleuri is used widely for the treatments of influenza, fever, inflammation, etc. in China, Japan and Korea. As a bulk herb, this herbal medicine occupies a comparatively big market share and is widely cultivated. However, because of the vigorous flowering and fruiting, the root growth is influenced seriously. ResultsWe assessed the effect of the continuous inflorescence removal (CIR) on improving the yield and quality of Radix Bupleuri. The results showed that the taproot length, root head diameter, and lateral root numbers were significantly elevated by CIR. We also found an average 1.71-fold increase in root biomass, and the lower lignin and higher saponin a content were detected in roots of CIR-treated plants. Based on a comparative transcriptome analysis, 172, 243, 1974 and 3024 differentially expressed genes (DEGs) were respectively identified in the CIR-treated roots. Gene Ontology and KEGG pathway functional annotation analysis of the DEGs showed that multiple genes were involved sugars metabolism pathway, lignin and terpenoids biosynthesis pathway, and the plant hormones signal transduction pathway. In addition, 295 genes encoding transcription factors (TFs), including members of the ERF, MYB-related, bHLH, NAC, MYB, AUX/IAA and bZIP families etc, were identified as CIR responsive. ConclusionThe CIR treatment could improve the yield, quality and market value of B. chinense DC. medicine, and the first transcriptome profile of CIR-treated roots of B. chinense DC. provides a series of clues of the molecular mechanisms of these phenotype changes induced by CIR.

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Section: Discussionmentioning

confidence: 82%

Continuous inflorescence removal changes parameters, lignin and saponin a content and alters the transcriptome of root of Bupleurum chinense DC

Wang

Zhang

Gao

et al. 2020

Preprint

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“…Due to the different prediction method, there were considerable discrepancy, but the AtUXSs in cytoplasm were ties specific function. Similar to AtUXS genes, antisense-expressed GhUXS3 gene, which was located in cytoplasm, could reduce xylose content in stem in Arabidopsis [14]. GhD08G085500 and GhD12G134200, predicted in cytoplasm, had higher expression.…”

Section: Discussionmentioning

confidence: 94%

“…Also, rice and populous genes were cloned [11,12]. In G. hirsutum, three GhUXS genes expression suggests UXS may play important roles in cotton fiber development [13,14].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification, phylogenetic and expressional analysis of the UXS gene family in cotton

Pan¹,

Wang²,

Wang³

et al. 2020

Preprint

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Background: UDP-glucuronate decarboxylase (UXS) is an enzyme in plants and participates in cell wall noncellulose. Previous research suggested that cotton GhUXS gene regulated the conversion of non-cellulosic polysaccharides and modulates their composition in plant cell walls, showing its possible cellular function determining the quality of cotton fibers. Here, we performed evolutionary, phylogenetic, and expressional analysis of UXS genes from cottons and other selected plants. Results: By exploring the sequenced cotton genomes, we identified 10, 10, 18, and 20 UXSs genes in Gossypium raimondii , Gossypium arboretum , Gossypium hirsutum and Gossypium barbadense , and retrieved their homologs from other representative plants, including 5 dicots, 1 monocot, 5 green alga, 1 moss, and 1 lycophyte. Phylogenetic analysis suggested that UXS genes could be divided into four subgroups and members within each subgroup shared similar exon-intron structures, motif and subcellular location. Notably, gene colinearity information indicates 100% constructed trees to have aberrant topology, and helps determine and use corrected phylogeny. In spite of conservative nature of UXS, during the evolution of Gossypium , UXS genes were subjected to significant positive selection on key evolutionary nodes. Expression profiles derived from RNA-seq data showed distinct expression patterns of GhUXS genes in various tissues and different development. Most of GhUXS gene expressed highly at 10, 20 and 25 DPA (day post anthesis) of fibers. Real-time quantitative PCR analysis GhUXS genes expressed highly at 20 DPA or 25 DPA. Conclusions: UXS is relatively conserved in plants and significant positive selection affects cotton UXS evolution. The comparative genome-wide identification and expression profiling would lay an important foundation to understanding the biological functions of UXS gene family in cotton species and other plants.

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Antisense expression of Gossypium hirsutum UDP-glucuronate decarboxylase in Arabidopsis leads to changes in cell wall components

Cited by 2 publications

References 29 publications

Continuous inflorescence removal changes parameters, lignin and saponin a content and alters the transcriptome of root of Bupleurum chinense DC

Continuous inflorescence removal changes parameters, lignin and saponin a content and alters the transcriptome of root of Bupleurum chinense DC

Genome-wide identification, phylogenetic and expressional analysis of the UXS gene family in cotton

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