Antisense‐mediated Depletion of Tomato Endoplasmic Reticulum Omega‐3 Fatty Acid Desaturase Enhances Thermal Tolerance

Wang, Huasen; Chang, Yu; Tang, Xianfeng; Wang, Liyan; Dong, Xun-Chun; Meng, Qingwei

doi:10.1111/j.1744-7909.2010.00957.x

Cited by 44 publications

(25 citation statements)

References 40 publications

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“…GO Biological Process is helpful for functional classification of analyzed genes. Terms “response to abiotic or biotic stimulus” and “response to stress” are enriched (Figure 2C), which is consistent with the previous studies showing that wax and cutin are protective tissues for environmental stress and unsaturated FAs play important roles in various stresses (Samuels et al 2008; Upchurch 2008; Wang et al 2010). More detailed analysis of developmental process showed that the identified co‐expressed genes are involved in floral organs (ovule, stamen and carpel) and whorl development, shoot and leaf morphogenesis, cuticle development and post‐embryonic development (details in Table 2).…”

Section: Resultssupporting

confidence: 90%

Co‐expression Analysis Identifies CRC and AP1 the Regulator of Arabidopsis Fatty Acid Biosynthesis

Han

Yin

Hou

2012

JIPB

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Fatty acids (FAs) play crucial rules in signal transduction and plant development, however, the regulation of FA metabolism is still poorly understood. To study the relevant regulatory network, fifty-eight FA biosynthesis genes including de novo synthases, desaturases and elongases were selected as "guide genes" to construct the co-expression network. Calculation of the correlation between all Arabidopsis thaliana (L.) genes with each guide gene by Arabidopsis co-expression dating mining tools (ACT) identifies 797 candidate FA-correlated genes. Gene ontology (GO) analysis of these co-expressed genes showed they are tightly correlated to photosynthesis and carbohydrate metabolism, and function in many processes. Interestingly, 63 transcription factors (TFs) were identified as candidate FA biosynthesis regulators and 8 TF families are enriched. Two TF genes, CRC and AP1, both correlating with 8 FA guide genes, were further characterized. Analyses of the ap1 and crc mutant showed the altered total FA composition of mature seeds. The contents of palmitoleic acid, stearic acid, arachidic acid and eicosadienoic acid are decreased, whereas that of oleic acid is increased in ap1 and crc seeds, which is consistent with the qRT-PCR analysis revealing the suppressed expression of the corresponding guide genes. In addition, yeast one-hybrid analysis and electrophoretic mobility shift assay (EMSA) revealed that CRC can bind to the promoter regions of KCS7 and KCS15, indicating that CRC may directly regulate FA biosynthesis.

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Section: Resultssupporting

confidence: 90%

Co‐expression Analysis Identifies CRC and AP1 the Regulator of Arabidopsis Fatty Acid Biosynthesis

Han

Yin

Hou

2012

JIPB

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“…Many biotic and abiotic stresses (such as heat, cold, drought, salt, and wounding) can cause chloroplast damage in plants (Price et al 1989;Chang et al 2004;Gao et al 2007;Wang et al 2010;Zhao et al 2010;Gong et al 2014). Therefore, damage in chloroplasts can also cause similar responses to biotic and abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

Loss‐of‐function mutation of rice SLAC7 decreases chloroplast stability and induces a photoprotection mechanism in rice

Fan

Chen

et al. 2015

JIPB

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Plants absorb sunlight to power the photochemical reactions of photosynthesis, which can potentially damage the photosynthetic machinery. However, the mechanism that protects chloroplasts from the damage remains unclear. In this work, we demonstrated that rice (Oryza sativa L.) SLAC7 is a generally expressed membrane protein. Loss‐of‐function of SLAC7 caused continuous damage to the chloroplasts of mutant leaves under normal light conditions. Ion leakage indicators related to leaf damage such as H2O2 and abscisic acid levels were significantly higher in slac7‐1 than in the wild type. Consistently, the photosynthesis efficiency and Fv/Fm ratio of slac7‐1 were significantly decreased (similar to photoinhibition). In response to chloroplast damage, slac7‐1 altered its leaf morphology (curled or fused leaf) by the synergy between plant hormones and transcriptional factors to decrease the absorption of light, suggesting that a photoprotection mechanism for chloroplast damage was activated in slac7‐1. When grown in dark conditions, slac7‐1 displayed a normal phenotype. SLAC7 under the control of the AtSLAC1 promoter could partially complement the phenotypes of Arabidopsis slac1 mutants, indicating a partial conservation of SLAC protein functions. These results suggest that SLAC7 is essential for maintaining the chloroplast stability in rice.

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“…The omega‐3 fatty acid desaturase gene was also found to be involved in high temperature tolerance in transgenic tomato, which had reduced activity of endoplasmic reticulum (Wang et al. ).…”

Section: Genetic Manipulation Of Ht Using Transgenic Technologiesmentioning

confidence: 99%

Heat stress in crop plants: its nature, impacts and integrated breeding strategies to improve heat tolerance

2014

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Increasing severity of high temperature worldwide presents an alarming threat to the humankind. As evident by massive yield losses in various food crops, the escalating adverse impacts of heat stress (HS) are putting the global food as well as nutritional security at great risk. Intrinsically, plants respond to high temperature stress by triggering a cascade of events and adapt by switching on numerous stress-responsive genes. However, the complex and poorly understood mechanism of heat tolerance (HT), limited access to the precise phenotyping techniques, and above all, the substantial G 9 E effects offer major bottlenecks to the progress of breeding for improving HT. Therefore, focus should be given to assess the crop diversity, and targeting the adaptive/morpho-physiological traits while making selections. Equally important is the rapid and precise introgression of the HT-related gene(s)/QTLs to the heat-susceptible cultivars to recover the genotypes with enhanced HT. Therefore, the progressive tailoring of the heat-tolerant genotypes demands a rational integration of molecular breeding, functional genomics and transgenic technologies reinforced with the next-generation phenomics facilities.

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Antisense‐mediated Depletion of Tomato Endoplasmic Reticulum Omega‐3 Fatty Acid Desaturase Enhances Thermal Tolerance

Cited by 44 publications

References 40 publications

Co‐expression Analysis Identifies CRC and AP1 the Regulator of Arabidopsis Fatty Acid Biosynthesis

Co‐expression Analysis Identifies CRC and AP1 the Regulator of Arabidopsis Fatty Acid Biosynthesis

Loss‐of‐function mutation of rice SLAC7 decreases chloroplast stability and induces a photoprotection mechanism in rice

Heat stress in crop plants: its nature, impacts and integrated breeding strategies to improve heat tolerance

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